5 documents found, page 1 of 1
Membrane-translocating peptides and toxins: from nature to bedside
Rádis-Baptista, Gandhi; Kerkis, Alexandre; Prieto-Silva, Álvaro Rossan; Hayash, Mirian Akemi Furuie; Kerkisf, Irina; Yamane, TetsuoToday, different functional classes of bioactive peptides and toxins isolated from diverse sources of living organisms are known. In medicine, these polypeptides present the potential to be used structurally unmodified or to serve as templates for molecular design of improved derivatives. Here, we refer to members of three classes of remarkable peptides and toxins that act at the cell membranes level and membra...
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Cro...
Kerkis, Alexandre; Kerkis, Irina; Rádis-Baptista, Gandhi; Oliveira, Eduardo B.; Vianna-Morgante, Angela M.; Pereira, Lygia V.; Yamane, TetsuoHerein we report that crotamine, a small lysine- and cysteine-rich protein from the venom of the South American rattlesnake, can rapidly penetrate into different cell types and mouse blastocysts in vitro. In vivo, crotamine strongly labels cells from mouse bone marrow and spleen and from peritoneal liquid, as shown by fluorescent confocal laser-scanning microscopy. Nuclear localization of crotamine was observed...
Cytotoxic effects of crotamine are mediated through lysosomal membrane permeabi...
Hayashi, Mirian A.F.; Nascimento, Fábio D.; Kerkis, Alexandre; Oliveira, Vitor; Oliveira, Eduardo B.; Pereira, Alexandre; Rádis-Baptista, GandhiCrotamine, one of the main toxic components of Crotalus durissus terrificus venom, is a small non-enzymatic basic polypeptide, which causes hind limb paralysis and necrosis of muscle cells. It is well-known that several toxins penetrate into the cytosol through endocytosis, although in many cases the mechanism by which this occurs has not been fully investigated. Recently, using low concentrations of crotamine,...
Scaling-Up of Dental Pulp Stem Cells Isolated from Multiple Niches
Lizier, Nelson F.; Kerkis, Alexandre; Gomes, Cicera M.; Hebling, Josimeri [UNESP]; Oliveira, Camila F.; Caplan, Arnold I.; Kerkis, IrinaMade available in DSpace on 2013-08-28T14:12:40Z (GMT). No. of bitstreams: 1 WOS000305892100123.pdf: 1025211 bytes, checksum: f3114ad3d761d448ea4009979a8c8d68 (MD5); Made available in DSpace on 2013-09-30T18:31:32Z (GMT). No. of bitstreams: 2 WOS000305892100123.pdf: 1025211 bytes, checksum: f3114ad3d761d448ea4009979a8c8d68 (MD5) WOS000305892100123.pdf.txt: 56945 bytes, checksum: e71c3f4e9d87411582be429d4ea1669f...
Pluripotent stem cells as an in vitro model of neuronal differentiation
Kerkis, Alexandre