7 documents found, page 1 of 1
Bacteriophages to tackle Alzheimer´s disease
Martins, Ivone M.; Lima, Alexandre Ferreira; Oliveira, Tiago Gil; Azeredo, Joana; Kessels, Helmut W.The first stages of Alzheimers disease (AD) pathology involve the formation of toxic amyloid beta (A) oligomers, which can begin to form up to two decades before symptoms and other known disease markers appear. The ability to detect A oligomers would allow early diagnosis, leading to improved disease management, care and prognosis through the use of therapeutic strategies targeting A before irreparable brain da...
M13 bacteriophages displaying peptide motifs targeting amyloid- eta, methods an...
Martins, Ivone M.; Azeredo, Joana; Kessels, Helmut W.; Gomes, Cláudio M.; Cristóvão, Joana S.The present disclosure relates to an engineered M13 bacteriophage displaying amyloidogenic peptide motifs from amyloid beta 42 (A42) at its surface. The present disclosure further relates to the use of the disclosed engineered M13 bacteriophage for detecting early species of A, namely oligomeric and fibrillar A, and preventing its aggregation promoting the inhibition of the progression of Alzheimer's disease an...
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in...
Martins, Ivone M.; Lima, Alexandre; de Graaff, Wim; Cristóvão, Joana S.; Brosens, Niek; Aronica, Eleonora; Kluskens, Leon; Gomes, Cláudio M.Oligomeric clusters of amyloid- (A) are one of the major biomarkers for Alzheimers disease (AD). However, proficient methods to detect A-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying A-derived peptides on their surface preferentially interact with A-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-...
Detection of amyloid-beta oligomers in early Alzheimer´s disease
Lima, Alexandre; de Graaff, Wim; Aronica, Eleonora; Azeredo, Joana; Kessels, Helmut W.; Martins, Ivone M.Amyloid-beta (A) seems to play a central role in Alzheimers disease (AD) pathology. During disease progression A peptides aggregate into various structures including soluble oligomers and fibrils, which ultimately deposit in the brain as insoluble plaques. While the plaques are a well-known hallmark of AD, recent findings revealed that even before plaque formation the still-soluble A oligomers are already able ...
Bacteriophages as a smart tool to target Alzheimer's Disease
Martins, Ivone M.; Resende, Mariana; Silva, Ana Isabel M.; Lodder, Tessa; Cristóvão, Joana; Gomes, Cláudio M.; Azeredo, Joana; Kessels, Helmut W.Alzheimer's disease is an irreversible, progressive and age-dependent neurodegenerative disorder affecting millions of people worldwide. In our brain, a small sized amyloid-beta (Abeta) peptide, when in excess, progressively aggregates into soluble oligomers and fibrils, to finally deposit and accumulate as insoluble amyloid plaques. The still-soluble Abeta oligomers and fibrils are sufficient to corrupt synapt...
A bacteriophage-based platform for early diagnosis of Alzheimers disease
Silva, Ana Isabel M.; Azeredo, Joana; Kessels, Helmut W.; Martins, Ivone M.[Excerpt] Alzheimer’s disease (AD) is the most common neurodegenerative disease affecting a large proportion of the human population worldwide. One hallmark of AD is the increased deposition of plaques, which consist of amyloid-beta (AB) peptide, a key molecule to cause AD onset and progression. However, it is not AB immobilized in plaques, but in the still-soluble oligomeric/fibrillar form that impairs synapti...
Engineering of specific bacteriophages for early diagnosis of Alzheimer′s disease
Martins, Ivone M.; Silva, Ana; Kessels, Helmut W.; Kluskens, LeonAlzheimer’s disease (AD) is the most common neurodegenerative disease affecting a large proportion of the human population worldwide with great impact on social and economic level. At molecular level, AD is characterized by an increased deposition of plaques, which consist of amyloid-beta however, it is not the amyloid-beta in plaques, but amyloid-beta in soluble oligomeric form that impairs synaptic function a...