2 documents found, page 1 of 1
Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (...
Nery, Flávia M. B.; Melo, Fernando Lucas; Boiteux, Leonardo Silva; Ribeiro, Simone G.; Resende, Renato de Oliveira; Orílio, Anelise FrancoIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, speci...
Expression in Escherichia coli, purification, refolding and antifungal activity...
Campos, Magnólia de Araújo; Silva, Marília Santos; Magalhães, Cláudio P .; Ribeiro, Simone G.; Del Sarto, Rafael Perseghini; Vieira, Eduardo A.Background: Heterologous protein expression in microorganisms may contribute to identify and demonstrate antifungal activity of novel proteins. The Solanum nigrum osmotin-like protein (SnOLP) gene encodes a member of pathogenesis-related (PR) proteins, from the PR-5 sub-group, the last comprising several proteins with different functions, including antifungal activity. Based on deduced amino acid sequence of Sn...