23 documents found, page 1 of 3
Ultrastructural characterization of the spermatozoa of Aethalion reticulatum Li...
Araújo, Vinícius Albano; Moreira, Jane; Neves, Clóvis Andrade; Lino-Neto, José; Báo, Sônia NairThe Hemiptera order is currently divided into four suborders. Among them the Auchenorrhyncha suborder is considered to be paraphyletic. Morphology of insect spermatozoa has provided promising characteristics that can be used for phylogenetic inference. In this study, Aethalion reticulatum (Aethalionidae) spermatozoa were examined by light and electron microscopy. The head of the spermatozoa is composed of an ac...
Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered ...
Paiva, Aline Dias; Fernandes, Kenner Morais; Dias, Roberto Sousa; Rocha, Alípio dos Santos; Oliveira, Leandro Licursi de; Neves, Clóvis AndradeBovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were evaluated. BALB/c mice were divided into three groups: negative control (NC group); mice given purified b...
FMRFamide-like midgut endocrine cells during the metamorphosis in Melipona quad...
Neves, Clóvis Andrade; Bhering, Leonardo Lopes; Serrao, Jose Eduardo; Gitirana, Lycia BritoThe FMRFamide, gastrin and cholecystokinin (CCK) occurrence in endocrine cells of insects has been described by several authors, although their functions are still not well defined for this group of animals. In the present study, the occurrence of endocrine cells producing FMRFamide, gastrin 1 and CCK-8 in the midgut (ventriculus) of Melipona quadrifasciata anthidioides (Hymenoptera, Apidae), before, during and...
Mesenchymal stem cells promote augmented response of endogenous neural stem cel...
Araujo, Marta Rocha; Carvalho, Pablo Herthel; Paula, Taís Silva de; Okano, Bárbara Silva; Carlo, Ricardo Junqueira Del; Neves, Clóvis AndradeA lesão medular resulta em déficits neurológicos graves, a maioria irreversíveis. A terapia celular representa uma estratégia para o tratamento, especialmente com a utilização de células-tronco, com resultados satisfatórios em vários modelos experimentais. O objetivo do estudo foi comparar o tratamento de lesões da medula espinal (SCI), com e sem o uso de células-tronco mesenquimais (MSC), para investigar se as...
Sperm storage in Hemidactylus mabouia: morphological and ultrastructural aspect...
Sartori, Sirlene Souza Rodrigues; Nogueira, Katiane de Oliveira Pinto Coelho; Araújo, Vinícius Albano; Neves, Clóvis Andrade; Kolisnyk, BenjaminSperm storage is a common phenomenon in most female reptiles. Evidence of sperm storage is based on the observation that female fertilization occurs even when females are separated from males, as well as the presence of agglomerates of spermatozoa in specific regions of the oviducts. Lizards are capable of storing sperm in the uterine tube, vagina, or in both regions. However, representatives of the Gekkonidae ...
Aedes aegypti midgut remodeling during metamorphosis
Fernande, Kenner Morais; Neves, Clóvis Andrade; Serrão, José Eduardo; Martins, Gustavo FerreiraThe Aedes aegypti midgut is restructured during metamorphosis; its epithelium is renewed by replacing the digestive and endocrine cells through stem or regenerative cell differentiation. Shortly after pupation (white pupae) begins, the larval digestive cells are histolized and show signs of degeneration, such as autophagic vacuoles and disintegrating microvilli. Simultaneously, differentiating cells derived fro...
Phagocytosis of spermatozoa by epithelial cells in the vagina of the lizard Hem...
Nogueira, Katiane de Oliveira Pinto Coelho; Araújo, Vinícius Albano; Sartori, Sirlene Souza Rodrigues; Neves, Clóvis AndradeHemidactylus mabouia is an Africa oviparous lizard that is now distributed on other continents and has been introduced to Brazil. In the majority of reptiles, the females have the ability to store spermatozoa in specialized regions of the genital tract. Considering that in H. mabouia the storage of spermatozoa is restricted to the region of the uterine tube, in this study we utilized optical and transmission el...
Digestive cells in the midgut of Triatoma vitticeps (Stal, 1859) in different s...
Rocha, Lamara Laguardia Valente; Neves, Clóvis Andrade; Zanuncio, José Cola; Serrão, José ÉduardoTriatoma vitticeps (Stal, 1859) is a hematophagous Hemiptera that, although being considered wild, can be found in households, being a potential Chagas’ disease vector. This work describes the histology and ultrastructure of the midgut of T. vitticeps under different starvation periods. Fifteen adults of both sexes starved for 3, 7, 20 and 25 days were studied. In general, digestive cells had apical microvilli,...
The regenerative cells during the metamorphosis in the midgut of bees
Martins, Gustavo Ferreira; Neves, Clóvis Andrade; Oliveira Campos, Lúcio Antonio; Serrão, José EduardoThe midgut epithelium of bees is formed by the digestive cells, responsible for enzyme secretion and nutrient absorption and for small regenerative cells that are placed in nests scattered among the digestive cells. During metamorphosis, the larval midgut epithelium degenerates and a new adult midgut epithelium is built during larval differentiation of regenerative cells. The present work focuses on the midgut ...
Use of fluorescence in a modified disector method to estimate the number of myo...
Novaes, Rômulo Dias; Penitente, Arlete Rita; Talvani, André; Natali, Antônio José; Neves, Clóvis Andrade; Maldonado, Izabel Regina Santos CostaConventional disector methods currently require considerable financial, technical and operational costs to estimate the number of cells, including cardyomyocytes, in a 3D area. To use fluorescence microscopy in a modified disector method to determine the number of myocytes in cardiac tissue in normal and pathological conditions. The study employed four-month-old male Wistar rats with weight of 366.25 ± 88.21g r...