1,3-propanediol (1,3-PDO) was produced from glycerol in three parallel Expanded Granular Sludge Blanket (EGSB) reactors inoculated with granular sludge (control reactor-R1), heat-treated granular sludge (R2) and disrupted granular sludge (R3) at Hydraulic Retention Times (HRT) between 3 and 24 h. Maximum 1,3-PDO yield (0.52 mol mol-1) and productivity (57 g L-1 d-1) were achieved in R1 at HRTs of 12 h and 3 h, ...
High production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impurities present in this substrate and/or the concentration of glycerol itself can affect the microbial metabolism. In this work, the effect of crude glycerol concentration on the production of butanol and 1,3-propanediol (1,3-PDO) by Clos...
1,3-propanediol (1,3-PDO) was produced from glycerol in three parallel Expanded Granular Sludge Blanket (EGSB) reactors inoculated with granular sludge (control reactor-R1), heat-treated granular sludge (R2) and disrupted granular sludge (R3) at Hydraulic Retention Times (HRT) between 3 and 24 h. Maximum 1,3-PDO yield (0.52 mol mol-1) and productivity (57 g L-1 d-1) were achieved in R1 at HRTs of 12 h and 3 h, ...
Two pre-treatments (granules disruption and heat) were applied to granular sludge to favour the production of 1,3-propanediol (1,3-PDO) from glycerol by non-methanogenic bacteria in expanded granular sludge blanket (EGSB) reactors. Different hydraulic retention times (HRT) were tested to achieve a maximum yield of 0.59 mol of 1,3-PDO/mol of glycerol in the control (no treatment) reactor at an HRT of 12 h. Molec...
Tese doutoramento do Programa Doutoral em Bioengenharia; The increasing demand for use of renewable resources as feedstock for the production of chemicals combined with advances in biotechnology is generating a renewed interest in the fermentative production of n-butanol and 1,3-propanediol (1,3-PDO). In this context, glycerol, a by-product of biodiesel and ethanol production, arises as a potential substrate fo...
Increasing demand for the use of renewable resources as feedstock for the production of chemicals combined with advances in biotechnology is generating a renewed interest in fermentative butanol production. In this sense, glycerol that is a byproduct of biodiesel production arises as a potential substrate for butanol production. In this work, Clostridium pasteurianum DMS 525 has been studied for butanol product...
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