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Anaerobic granular sludge as a biocatalyst for 1,3-propanediol production from ...

Gallardo, R.; Faria, Cristiana; Rodrigues, L. R.; Alves, M. M.

1,3-propanediol (1,3-PDO) was produced from glycerol in three parallel Expanded Granular Sludge Blanket (EGSB) reactors inoculated with granular sludge (control reactor-R1), heat-treated granular sludge (R2) and disrupted granular sludge (R3) at Hydraulic Retention Times (HRT) between 3 and 24 h. Maximum 1,3-PDO yield (0.52 mol mol-1) and productivity (57 g L-1 d-1) were achieved in R1 at HRTs of 12 h and 3 h, ...


Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 to...

Gallardo, R.; Alves, M. M.; Rodrigues, L. R.

High production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impurities present in this substrate and/or the concentration of glycerol itself can affect the microbial metabolism. In this work, the effect of crude glycerol concentration on the production of butanol and 1,3-propanediol (1,3-PDO) by Clos...


Anaerobic granular sludge as a biocatalyst for 1,3-propanediol production from ...

Gallardo, R.; Faria, Cristiana; Rodrigues, L. R.; Pereira, M. A.; Alves, M. M.

1,3-propanediol (1,3-PDO) was produced from glycerol in three parallel Expanded Granular Sludge Blanket (EGSB) reactors inoculated with granular sludge (control reactor-R1), heat-treated granular sludge (R2) and disrupted granular sludge (R3) at Hydraulic Retention Times (HRT) between 3 and 24 h. Maximum 1,3-PDO yield (0.52 mol mol-1) and productivity (57 g L-1 d-1) were achieved in R1 at HRTs of 12 h and 3 h, ...


Production of 1,3-propanediol in EGSB reactors by open mixed cultures using gly...

Gallardo, R.; Faria, C.; Pereira, M. A.; Rodrigues, L. R.; Alves, M. M.

Two pre-treatments (granules disruption and heat) were applied to granular sludge to favour the production of 1,3-propanediol (1,3-PDO) from glycerol by non-methanogenic bacteria in expanded granular sludge blanket (EGSB) reactors. Different hydraulic retention times (HRT) were tested to achieve a maximum yield of 0.59 mol of 1,3-PDO/mol of glycerol in the control (no treatment) reactor at an HRT of 12 h. Molec...


New strategies for the production of butanol and 1,3-propanediol from glycerol

Gallardo, R.

Tese doutoramento do Programa Doutoral em Bioengenharia; The increasing demand for use of renewable resources as feedstock for the production of chemicals combined with advances in biotechnology is generating a renewed interest in the fermentative production of n-butanol and 1,3-propanediol (1,3-PDO). In this context, glycerol, a by-product of biodiesel and ethanol production, arises as a potential substrate fo...


New strategies for the production of butanol

Gallardo, R.

Increasing demand for the use of renewable resources as feedstock for the production of chemicals combined with advances in biotechnology is generating a renewed interest in fermentative butanol production. In this sense, glycerol that is a byproduct of biodiesel production arises as a potential substrate for butanol production. In this work, Clostridium pasteurianum DMS 525 has been studied for butanol product...



New strategies for the production of butanol

Gallardo, R.

Fundação para a Ciência e a Tecnologia (FCT)


New strategies for the production of butanol

Gallardo, R.; Alves, M. M.; Rodrigues, L. R.


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