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MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

Dueholm, Morten Kam Dahl; Andersen, Kasper Skytte; Petersen, Anne-Kirstine C.; Rudkjøbing, Vibeke; Alves, M. M.; Bajón-Fernández, Yadira

Anaerobic digestion represents a key biotechnology for the transformation of organic waste into renewable energy (biogas) and relies on complex microbial communities that work in concert to degrade the complex substrates into methane and carbon dioxide. Here, we sequenced more than half a million high-quality, full-length 16S rRNA gene sequences from 285 full-scale anaerobic digesters (ADs) across the world to ...


MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

Dueholm, Morten Kam Dahl; Andersen, Kasper Skytte; Korntved, Anne-Kirstine C.; Rudkjøbing, Vibeke; Alves, M. M.; Bajón-Fernández, Yadira

Anaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing of 285 full-scale anaerobic digesters (ADs) to expand our knowledge about diversity and function of the bacteria and archaea in ADs worldwide. The sequences are processed into full-length 16S rRNA amplicon sequence variants (FL-ASVs) and...


Towards a standardization of biomethane potential tests

Holliger, Christof; Alves, M. M.; Andrade, Diana; Angelidaki, Irini; Astals, Sergi; Baier, Urs; Bougrier, Claire; Buffière, Pierre; Carballa, Marta

Production of biogas from different organic materials is a most interesting source of renewable energy. The biomethane potential (BMP) of these materials has to be determined to get insight in design parameters for anaerobic digesters. Although several norms and guidelines for BMP tests exist, inter-laboratory tests regularly still show high variability of BMPs for the same substrate. A workshop was held in Jun...


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