6 documents found, page 1 of 1

Use of recombinant antigens for sensitive serodiagnosis of american tegumentary...

This work was supported by Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (grant 2015/22075-8 and fellowship 2016/02622-7 to C.M.S.), by CAPES (fellowship to C.M.S.), by Conselho Nacional de Pesquisa (research fellowship to H.G.), and by Laborat?rios de Investiga??o M?dica (LIM-38-HC, FMUSP). This research was also supported by funding from the Bill and Melinda Gates Foundation under grants OPP49932 and O...

LABORATORY DIAGNOSIS OF MAIN INFECTIOUS AND AUTOIMUNE DISEASES

Transfusion-transmitted malaria: case report of asymptomatic donor harboring Pl...

Malaria in Brazil is endemic in the Amazon region, but autochthonous cases with low parasitaemia occur in the Atlantic Forest area of the country. According to Brazilian legislation no test is mandatory for blood donors from non-endemic areas. However if they have traveled to malaria transmission regions they are deferred for six months before they can donate. This report describes a transfusion-transmitted mal...

Potential immunological markers for diagnosis and therapeutic assessment of tox...

In human toxocariasis, there are few approaches using immunological markers for diagnosis and therapeutic assessment. An immunoblot (IB) assay using excretory-secretory Toxocara canis antigen was standardized for monitoring IgG, IgE and IgA antibodies in 27 children with toxocariasis (23 visceral, three mixed visceral and ocular, and one ocular form) for 22-116 months after chemotherapy. IB sensitivity was 100%...

Malaria serology: performance of six Plasmodium falciparum antigen extracts and...

The study evaluated six Plasmodium falciparum antigen extracts to be used in the IgG and IgM enzyme-linked immunosorbent assays (ELISA), for malaria diagnosis and epidemiological studies. Results obtained with eighteen positive and nine negative control sera indicated that there were statistically significant differences among these antigen extracts (Multifactor ANOVA, p< 0.0001). Urea, sodium deoxycholate and ...

Human malaria: standardization of serologic tests for individual diagnostic and...

The indirect immunofluorescence antibody test (IFA) is normally employed as reference test in the serology of malaria. In this report we standardized and optimized the test, for our condition, utilizing P. falciparum obtained from human blood on culture and P. vivax obtained from human blood as antigens, for detection of IgG and IgM antibodies. Some technical variables, were tested and best resulto were obtaine...