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Fluorescent in situ hybridization of pre-incubated blood culture material for t...

Silva, Roberto Moreira da; Silva Neto, João Ricardo da; Santos, Carla Silvana; Cruz, Kátia Santana; Frickmann, Hagen; Poppert, Sven; Koshikene, Daniela

Fluorescence in situ hybridization (FISH) has been shown to be useful for the detection of Candida and Cryptococcus species in blood culture materials. FISH procedures for the detection of Histoplasma capsulatum var. capsulatum have not been reported so far. This study describes the development and evaluation of fluorescently labeled rRNA-targeting FISH probes to detect and identify H. capsulatum in blood cultu...

Date: 2020   |   Origin: Oasisbr

Estratégias germinativas de sete espécies florestais de diferentes estágios suc...

Koshikene, Daniela

O principal objetivo deste trabalho foi identificar estratégias germinativas distintas de sete espécies florestais em relação aos diferentes estágios sucessionais da várzea. Há uma ausência de consenso acerca da distribuição destas espécies ao longo dos gradientes sucessionais nas florestas de várzea, como se segue: Junk (1989) classificou Salix martiana como pertencente à comunidade arbustiva baixa, enquanto W...

Date: 2020   |   Origin: Oasisbr

Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fu...

Silva, Roberto Moreira da; Silva Neto, João Ricardo da; Santos, Carla Silvana; Frickmann, Hagen; Poppert, Sven; Cruz, Kátia Santana; Koshikene, Daniela

The aim of this study was to evaluate the diagnostic performance of in-house FISH (fluorescence in situ hybridisation) procedures for the direct identification of invasive fungal infections in blood cultures and cerebrospinal fluid (CSF) samples and to compare these FISH results with those obtained using traditional microbiological techniques and PCR targeting of the ITS1 region of the rRNA gene. In total, 112 ...

Date: 2020   |   Origin: Oasisbr

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