50 documents found, page 1 of 5
Valorisation of olive oil by-products into pectic- and glucuronoxylo-oligosacch...
Cordeiro, Ana; Fernandes, Andreia; Ferreira, Andreia S.; Coelho, Elisabete; Coimbra, Manuel A.; Silvério, Sara C.; Cadavez, Vasco; Peres, António M.Olive pomace (OLP) and stones (OLS) are key by-products of olive oil production, rich in lignocellulose and pectin, making them viable substrates for prebiotic oligosaccharide (OS) production. This study evaluated the chemical composition of OLP and OLS powders (OLPp and OLSp) and their potential for OS production through one-step fermentation using recombinant Bacillus subtilis 3610. Both substrates had compar...
Fodinibius alkaliphilus sp. nov., a moderately halophilic and alkaliphilic bact...
He, Yang; Simões, Marta Filipa Jesus Freitas; de la Haba, Rafael R.; Santos-Pereira, Cátia; Pereira-Sousa, Joana; Gomes, Joana S.A novel moderately halophilic and alkaliphilic Gram-stain-negative, strictly aerobic, bacterial strain (N2T) was isolated from an inland saltern in central Portugal. The taxonomic position of this isolate was determined based on polyphasic taxonomic and phylogenomic analysis. Phylogenetic analysis based on 16S rRNA gene sequences indicated that isolate N2T belongs to the genus Fodinibius, showing the highest si...
Paper-based biomimetic test-strip for CA15-3 with coloured readout
Carneiro, Mariana C. C. G.; Rodrigues, Lígia R.; Moreira, Felismina; Sales, M. Goreti F.The combination of molecularly-imprinted polymers (MIPs) on paper substrates for capturing a cancer antigen 15–3 (CA15-3) with the traditional coloured transduction scheme of enzyme-linked immunosorbent assay (ELISA) using 3,3′,5,5′-tetramethylbenzidine (TMB), horseradish peroxidase (HRP) and H2O2 is presented here for the first time. Here, the paper surface was modified with a silane derivative containing an a...
Epilactose as a promising butyrate-promoter prebiotic via microbiota modulation
Cardoso, Beatriz B.; Amorim, Cláudia Catarina Oliveira; Franco-Duarte, Ricardo; Alves, Joana I.; Barbosa, Sónia G.; Silvério, Sara Isabel CruzEpilactose is a disaccharide composed of galactose and mannose, and it is currently considered an under development prebiotic. In this study, we described the prebiotic potential of epilactose by in vitro fermentation using human fecal inocula from individuals following a Mediterranean diet (DM) or a Vegan diet (DV). The prebiotic effect of epilactose was also compared with lactulose and raffinose, and interest...
Heterologous expression and structure prediction of a xylanase identified from ...
Sousa, Joana; Santos-Pereira, Cátia; Dias, Joana Sofia Gomes; Costa, Ângela Maria Araújo; Santos, Andréia O.; Franco-Duarte, RicardoXylanases are key biocatalysts in the degradation of the 1,4glycosidic linkages in the xylan backbone of hemicellulose. These enzymes are potentially applied in a wide range of bioprocessing industries under harsh conditions. Metagenomics has emerged as powerful tools for the bioprospection and discovery of interesting bioactive molecules from extreme ecosystems with unique features, such as high temperatures. ...
Stepbystep optimization of a heterologous pathway for de novonaringenin product...
Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Rodrigues, Lígia R.Naringenin is a plant polyphenol, widely explored due to its interesting biological activities, namely anticancer, antioxidant, and anti-inflammatory. Due to its potential applications and attempt to overcome the industrial demand, there has been an increased interest in its heterologous production. The microbial biosynthetic pathway to produce naringenin is composed of tyrosine ammonia-lyase (TAL), 4-coumarate...
Different approaches to Olive Pomace valorisation
Cordeiro, Ana; Lamas, Sandra; Rodrigues, Ana; Cadavez, Vasco; Silvério, Sara; Peres, António M.; Rodrigues, Lígia R.; Amorim, CláudiaOlive pomace (OP) is a substantial by-product, rich in lignocellulosic materials and water, obtained following the extraction of olive oil. It comprises the following components: olive skins, pulp, seeds, and stones. As global olive oil production continues to rise, the management and utilisation of OP have become increasingly important from both an economic and an environmental perspective [1]. In this study, ...
Assessing the prebiotic potential of xylooligosaccharides produced by one-step ...
Cordeiro, Ana; Fernandes, Andreia; Sousa, Joana; Cardoso, Beatriz; Alves, Joana; Silvério, Sara; Peres, António M.; Rodrigues, Lígia R.; Amorim, CláudiaA prebiotic is defined as a substrate that is selectively utilized by host microrganisms, conferring a health benefit
Olive oil by-products as potential alternative substrates for xylooligosacchari...
Cordeiro, Ana; Fernandes, Andreia; Peres, António M.; Rodrigues, Lígia R.; Amorim, CláudiaOlive pomace (OP) and olive stones (OS) are industrial by-products from the olive oil production. These residues have a high percentage of cellulose, hemicellulose, and lignin, which makes them a good source of fermentable sugars and xylan, as well as a potential alternative substrate for xylooligosaccharides (XOS) production. In this work, OP and OS were chemically characterized and used for the first time as ...
Assessing the prebiotic potential of xylooligosaccharides produced by one-step ...
Cordeiro, Ana; Fernandes, Andreia; Sousa, Joana; Cardoso, Beatriz; Alves, Joana; Silvério, Sara; Peres, António M.; Rodrigues, Lígia R.; Amorim, CláudiaThe prebiotic effect is a fundamental concept in the fields of nutrition and gut health, referring to the beneficial effects of specific non-digestible dietary components on the gut microbiota, including xylooligosaccharides (XOS). These compounds function as food sources for beneficial gut bacteria, fostering their growth and activity. In this work, in vitro studies were performed to evaluate the prebiotic pot...