8 documents found, page 1 of 1
Making waves
Alygizakis, Nikiforos; Ng, Kelsey; Čirka, Ľuboš; Berendonk, Thomas; Cerqueira, Francisco; Cytryn, Eddie; Deviller, Geneviève; Fortunato, GianuarioWith the global concerns on antibiotic resistance (AR) as a public health issue, it is pivotal to have data exchange platforms for studies on antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in the environment. For this purpose, the NORMAN Association is hosting the NORMAN ARB&ARG database, which was developed within the European project ANSWER. The present article provides an overview...
MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters
Dueholm, Morten Kam Dahl; Andersen, Kasper Skytte; Petersen, Anne-Kirstine C.; Rudkjøbing, Vibeke; Alves, M. M.; Bajón-Fernández, YadiraAnaerobic digestion represents a key biotechnology for the transformation of organic waste into renewable energy (biogas) and relies on complex microbial communities that work in concert to degrade the complex substrates into methane and carbon dioxide. Here, we sequenced more than half a million high-quality, full-length 16S rRNA gene sequences from 285 full-scale anaerobic digesters (ADs) across the world to ...
MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters
Dueholm, Morten Kam Dahl; Andersen, Kasper Skytte; Korntved, Anne-Kirstine C.; Rudkjøbing, Vibeke; Alves, M. M.; Bajón-Fernández, YadiraAnaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing of 285 full-scale anaerobic digesters (ADs) to expand our knowledge about diversity and function of the bacteria and archaea in ADs worldwide. The sequences are processed into full-length 16S rRNA amplicon sequence variants (FL-ASVs) and...
A global multinational survey of cefotaxime-resistant coliforms in urban wastew...
Marano, Roberto B. M.; Fernandes, Telma; Manaia, Célia M.; Nunes, Olga; Morrison, Donald; Berendonk, Thomas U.; Kreuzinger, Norbert; Tenson, TanelThe World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost...
A global multinational survey of cefotaxime-resistant coliforms in urban wastew...
Marano, Roberto B.M.; Fernandes, Telma; Manaia, Célia M.; Nunes, Olga; Morrison, Donald; Berendonk, Thomas U.; Kreuzinger, Norbert; Tenson, TanelThe World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost...
Antibiotic resistance genes in treated wastewater and in the receiving water bo...
Cacace, Damiano; Fatta-Kassinos, Despo; Manaia, Célia M.; Cytryn, Eddie; Kreuzinger, Norbert; Rizzo, Luigi; Karaolia, Popi; Schwartz, ThomasThere is increasing public concern regarding the fate of antibiotic resistance genes (ARGs) during wastewater treatment, their persistence during the treatment process and their potential impacts on the receiving water bodies. In this study, we used quantitative PCR (qPCR) to determine the abundance of nine ARGs and a class 1 integron associated integrase gene in 16 wastewater treatment plant (WWTP) effluents f...
High throughput analysis of integron gene cassettes in wastewater environments
Gatica, Joao; Tripathi, Vijay; Green, Stefan; Manaia, Célia M.; Berendonk, Thomas; Cacace, Damiano; Merlin, Christophe; Kreuzinger, NorbertIntegrons are extensively targeted as a proxy for anthropogenic impact in the environment. We developed a novel high-throughput amplicon sequencing pipeline that enables characterization of thousands of integron gene cassette-associated reads, and applied it to acquire a comprehensive overview of gene cassette composition in effluents from wastewater treatment facilities across Europe. Between 38 100 and 172 99...
Tackling antibiotic resistance: the environmental framework
Berendonk, Thomas U.; Manaia, Célia M.; Merlin, Christophe; Fatta‑Kassinos, Despo; Cytryn, Eddie; Walsh, Fiona; Bürgmann, Helmut; Sørum, HenningAntibiotic resistance is a threat to human and animal health worldwide, and key measures are required to reduce the risks posed by antibiotic resistance genes that occur in the environment. These measures include the identification of critical points of control, the development of reliable surveillance and risk assessment procedures, and the implementation of technological solutions that can prevent environment...