122 documents found, page 1 of 13
MicroVasculoid-chip: a 3D self-assembled human microcirculation-on-a-chip model...
Abreu, Catarina M.; Lima, Ana Cláudia Fernandes; Neves, N. M.; Kundu, Subhas C; Reis, R. L.; Caballero, DavidThe microvasculature within the tumor microenvironment is crucial for the invasion and dissemination of cancer cells throughout the body. Given its importance and dynamic behavior, several microfluidic models have been developed to study microvascular infiltration and its interaction with cancer cells. However, most of these models primarily focus on blood vessels and use microfluidic channels coated with endot...
Patterned glycopeptide-based supramolecular hydrogel promotes the alignment and...
Castro, Vânia Isabel Baptista; Amorim, Sara; Caballero, David; Abreu, Catarina M.; Kundu, Subhas C; Reis, R. L.; Pashkuleva, I.; Pires, R. A.The functional restoration of a damaged cardiac tissue relies on a synchronized contractile capacity of exogenous and/or endogenous cardiomyocytes, which is challenging to achieve. Here, we explored the potential of the short glycopeptide diphenylalanine glucosamine-6-sulfate (FFGlcN6S) conjugated with an aromatic moiety, namely fluorenylmethoxycarbonyl (Fmoc), to enhance cardiac tissue regeneration. At physiol...
Nano-armed Limosilactobacillus reuteri for enhanced photo-immunotherapy and mic...
Xu, Haiting; Wang, Yajun; Liu, Ga; Zhu, Zhenhua; Shahbazi, Mohammad-Ali; Reis, R. L.; Kundu, Subhas C; Shi, Xiaoxiao; Zu, Menghang; Xiao, BoDespite being a groundbreaking approach to treating colorectal cancer (CRC), the efficacy of immunotherapy is significantly compromised by the immunosuppressive tumor microenvironment and dysbiotic intestinal microbiota. Here, leveraging the superior carrying capacity and innate immunity-stimulating property of living bacteria, a nanomedicine-engineered bacterium, LR-S-CD/CpG@LNP, with optical responsiveness, i...
Antheraea pernyi silk nanofibrils with inherent RGD motifs accelerate diabetic ...
Duan, Lian; Liu, Ga; Liao, Fuying; Xie, Chunyu; Shi, Jiahao; Yang, Xiao; Zheng, Fan; Reis, R. L.; Kundu, Subhas C; Xiao, BoThe chronic inflammation and matrix metalloprotease (MMP)-induced tissue degradation significantly disrupt re-epithelization and delay the healing process of diabetic wounds. To address these issues, we produced nanofibrils from Antheraea pernyi (Ap) silk fibers via a facile and green treatment of swelling and shearing. The integrin receptors on the cytomembrane could specifically bind to the Ap nanofibrils (Ap...
Injectable dual-network hydrogel for phototherapy and immunomodulation in the t...
Bao, Shengfei; Jiang, Aodi; Peng, Li; Liao, Fuying; Liu, Ga; Chen, Li; Zhou, Peng; Zhu, Zhen; Reis, R. L.; Kundu, Subhas C; Duan, Lian; Xiao, BoContinuous invasion and infection of drug-resistant bacteria leads to chronic inflammation and ulcers in skin wounds, which hinders wound healing and even results in sepsis. Here, we constructed a multifunctional injectable hydrogel (GA/PIOP/SF) that was composed of a synthesized porous ionic organic polymer (PIOP) with photothermal and photodynamic therapy (PDT/PTT) capacities to kill bacteria, a bioactive sma...
Reinforced plant-derived lipid nanoparticles for oral precise epigenome editing...
Gao, Qiang; Gao, Yunxia; Cao, Yingui; Xu, Haiting; Ma, Ya; Zu, Menghang; Yang, Qiang; Yang, Kun; Zhu, Zhenhua; Liu, Chao; Shi, X. X.; Reis, R. L.The clinical application of CRISPR-Cas9 remains limited by delivery challenges, particularly for oral administration. Lysine-specific demethylase 1 (Lsd1) plays a key role in colonic inflammation and tumorigenesis. Here, we developed an oral genome-editing platform (TPGS-RNP@LNP), where Lsd1-targeting ribonucleoproteins (RNPs) were encapsulated in mulberry leaf lipid nanoparticles (LNPs) and formulated with d-α...
Gelation dynamics, formation mechanism, functionalization, and 3D bioprinting o...
Fan, Linpeng; Cai, Zengxiao; Zhao, Jian; Mahmoudi, Negar; Wang, Yi; Cheeseman, Samuel; Aguilar, Lilith Caballero; Reis, R. L.; Kundu, Subhas CSilk fibroin (SF), derived from silk cocoon fibers (Bombyx mori), is a natural protein polymer known for its biocompatibility, biodegradability, and sustainability. The protein can be processed into various material formats suitable for a range of applications. Among these, SF hydrogels are useful in the biomedical field, such as tissue engineering, due to the tailorable structures and properties achievable thr...
Nanomaterials-based hybrid bioink platforms in advancing 3D bioprinting technol...
Chandra, Dilip Kumar; Reis, R. L.; Kundu, Subhas C; Kumar, Awanish; Mahapatra, Chinmaya3D bioprinting is recognized as the ultimate additive biomanufacturing technology in tissue engineering and regeneration, augmented with intelligent bioinks and bioprinters to construct tissues or organs, thereby eliminating the stipulation for artificial organs. For 3D bioprinting of soft tissues, such as kidneys, hearts, and other human body parts, formulations of bioink with enhanced bioinspired rheological ...
Silk fibroin hollow microneedle system for sustained transdermal administration...
Jia, Tianshuo; Geng, Yiyu; Shao, Huiyan; Tan, Guohongfang; Zhang, Xiaofeng; Kundu, Subhas C; Lu, ShenzhouLiraglutide demonstrates a glucose-dependent hypoglycemic mechanism with significant therapeutic potential for type 2 diabetes mellitus. This study developed a hollow microneedle system featuring dual drug loading mechanisms cavity encapsulated and matrix embedded to achieve sustained release of liraglutide. The cavity structure avoids direct contact between the drug and the silk fibroin shell, as well as the b...
Oral oncolytic magnetotactic bacteria elicit anti-colorectal tumor immunity and...
Jiang, Aodi; Li, Baoyi; Zu, Menghang; Chen, Li; Zhao, Chan; Tian, Jiesheng; Reis, R. L.; Kundu, Subhas C; Xiao, Bo; Shi, XiaoxiaoTherapeutic outcomes of colorectal cancer (CRC) are influenced by intestinal microbiota and metabolites. To leverage the tumor-tropism and microbiota-regulating properties of bacteria, we developed oncolytic magnetotactic bacteria (MSR-CPT/APPs) loaded with camptothecin (CPT) and anti-PD-L1 peptide (APP) for targeted chemo-magnetothermal immunotherapy of CRC. To further achieve oral delivery, MSR-CPT/APPs were ...