180 documents found, page 1 of 18
The comet assay as a tool in human biomonitoring exposure to combustion-derived...
Møller, Peter; Gajski, Goran; Gerić, Marko; Haveric, Anja; Stopper, Helga; Bankoglu, Ezgi Eyluel; Azqueta, Amaya; Giovannelli, Lisa; Collins, AndrewHumans are exposed to environmental or occupational air pollution from combustion emissions in outdoor and indoor environments. Irrespective of the sources, combustion emissions are characterized by being a complex mixture of particles, volatile compounds and gases. The present systematic review summarizes results on DNA strand breaks measured by the comet assay in leukocytes, from studies on human exposure to ...
The comet assay as a tool in human biomonitoring exposure to volatile organic c...
Giovannelli, Lisa; Møller, Peter; Gajski, Goran; Stopper, Helga; Azqueta, Amaya; Bankoglu, Ezgi Eyluel; Haveric, Anja; Gerić, Marko; Collins, AndrewExposure to volatile organic compounds (VOCs) such as benzene, styrene, toluene, and formaldehyde is associated with genotoxicity and increased risk of cancer. In this systematic review and meta-analysis, we have assessed the effects of VOCs exposure on levels of DNA strand breaks in leukocytes, measured by the comet assay, in human biomonitoring studies. The literature search led to 57 studies included in the ...
The comet assay as a tool in human biomonitoring exposure to anaesthetic gases:...
Gajski, Goran; Haveric, Anja; Møller, Peter; Azqueta, Amaya; Giovannelli, Lisa; Gerić, Marko; Stopper, Helga; Bankoglu, Ezgi Eyluel; Collins, AndrewAnaesthetic gases are agents used to induce and maintain general anaesthesia during surgical procedures. Common examples include sevoflurane, isoflurane, and desflurane, which act by depressing the central nervous system to produce unconsciousness and analgesia. These gases are administered through a vaporiser and inhaled via a mask or endotracheal tube. While effective, they can contribute to environmental pol...
A call for preregistration of in vitro research: pregistration as a tool to imp...
Heinl, Céline; Franco, Nuno H.; Bert, Bettina; Siewert, Katherina; Movia, Dania; Aruçi, Edlira; Ladeira, Carina; Sanin, Ahmed Y.; Neuhaus, WinfriedBiomedical research, like any other research field, is vulnerable to problems in its planning, execution, documentation, analysis, interpretation, and reporting. These issues can impede the reproducibility of research findings and hinder translation into clinical practice. In addition, there is growing societal pressure to hasten the shift to animal-free research methods. To alleviate the ethical dilemma of usi...
Carcinogenicity of tris(chloropropyl) phosphate, butyraldehyde, and cumyl hydro...
Lachenmeier, Dirk W.; Arrandale, Victoria H.; DeMarini, David M.; Ruksha, Tatiana; Abdallah, Mohamed A-E; Bettini, Giuliano; Ishii, YujiIn March 2026, a Working Group of 12 scientists from nine countries met at the International Agency for Research on Cancer (IARC) in Lyon, France, to finalise their evaluation of the carcinogenicity of tris(chloropropyl) phosphate (TCPP), butyraldehyde, and cumyl hydroperoxide. TCPP was classified as “probably carcinogenic to humans” (Group 2A) based on the combination of “sufficient” evidence for cancer in exp...
The comet assay as a tool in human biomonitoring exposure to antineoplastic dru...
Ladeira, Carina; Azqueta, Amaya; Giovannelli, Lisa; Gajski, Goran; Gerić, Marko; Haveric, Anja; Stopper, Helga; Bankoglu, Ezgi Eyluel; Collins, AndrewAntineoplastic agents are toxic compounds, generally used in the treatment of cancers, which are recognized as carrying a cancer development risk. In this systematic review and meta-analysis of human biomonitoring studies, we have assessed the effects of exposure to antineoplastic drugs on levels of DNA strand breaks in leukocytes, measured by the comet assay. Focusing on the application of the comet assay in h...
Substituição da formalina neutra a 10% por agave na fixação de material biológi...
Almeida, Beatriz; Silva, Joana; Maria, Mariana; Cunha, Gilda; Mendonça, Paula; Ladeira, CarinaA utilização de formaldeído na fixação de amostras biológicas apresenta riscos para a saúde humana e ambiente, devido à sua toxicidade e potencial carcinogénico, realçando a necessidade de encontrar alternativas mais seguras e sustentáveis. O objetivo deste estudo é testar o agave como fixador biológico, comparando os seus resultados com os da formalina neutra tamponada a 10% (FNT a 10%) em termos de preservaçã...
Substituição da formalina neutra a 10% por agave na fixação de material biológi...
Almeida, Beatriz; Silva, Joana; Maria, Mariana; Cunha, Gilda; Mendonça, Paula; Ladeira, CarinaA fixação de tecidos biológicos é essencial para preservar a estrutura celular ao evitar a autólise. O formaldeído, comummente usado sob a forma de formalina (solução aquosa de 37-40% de formaldeído), é o mais usado, por ter baixo custo e grande poder de penetração nos tecidos, com artefactos de retração reduzidos, no entanto apresenta riscos para a saúde humana e ambiente, devido à sua toxicidade e potencial c...
Comparação entre a parafina e a cera de abelha na impregnação e inclusão da téc...
Alves, Joana; Almeida, Rita; Palma, João; Ladeira, CarinaA parafina é o meio de impregnação e inclusão de eleição devido às suas características inerentes e baixo custo, no entanto é uma substância que apresenta problemas ambientais e técnicos. Na tentativa de combater estas lacunas, e tendo em conta os resultados obtidos por Antony et al em tecidos orais e as suas semelhanças com a parafina, a cera de abelha surge como uma alternativa promissora. Objetivo do estudo:...
Genotoxicity and cytotoxicity assessment of volatile organic compounds in patho...
Baptista, Fátima; Garcia, Patrícia V.; Rodrigues, Armindo S.; Ladeira, CarinaIn pathology laboratories, several volatile organic compounds (VOCs) are used, such as formaldehyde, ethanol, and xylene. These substances are recognized as genotoxic and cytotoxic, which is why their handling poses risks to human health. The buccal micronucleus (MN) cytome assay is a non-invasive, useful, and simple method to detect these effects in exposed individuals. The study aimed to evaluate the risk of ...