2 documents found, page 1 of 1
Nucleolin Overexpression Predicts Patient Prognosis While Providing a Framework...
Fernandes, Ângela Valério; Fonseca, Nuno A.; Gonçalves, Nélio; Cruz, Ana F.; Pereira, Marta I.; Gregório, Ana C.; Moura, Vera; Ladeirinha, Ana F.Notwithstanding the advances in the treatment of lung cancer with immune checkpoint inhibitors, the high percentage of non-responders supports the development of novel anticancer treatments. Herein, the expression of the onco-target nucleolin in patient-derived pulmonary carcinomas was characterized, along with the assessment of its potential as a therapeutic target. The clinical prognostic value of nucleolin f...
Analytical Approaches toward Successful Human Cell Metabolome Studies by NMR Sp...
Duarte, Iola F.; Marques, Joana; Ladeirinha, Ana F.; Rocha, Cláudia; Lamego, Inês; Calheiros, Rita; Silva, Tânia M.; Marques, M. Paula M.The aim of this work was to investigate the effects of cell handling and storage on cell integrity and 1H high resolution magic angle spinning (HRMAS) NMR spectra. Three different cell types have been considered (lung tumoral, amniocytes, and MG-63 osteosarcoma cells) in order for sample-dependent effects to be identified. Cell integrity of fresh cells and cells frozen in cryopreservative solution was 70−80%, w...