49 documents found, page 1 of 5
In vitro and in vivo assessment of nanoceria biocompatibility for their safe us...
Fernández-Bertólez, N; Martínez, L; Ramos-Pan, L; Touzani, A; Costa, C; Laffon, B; Valdiglesias, VNanoceria, or cerium dioxide nanoparticles (CeO2 NP), are increasingly employed in a number of industrial and commercial applications. Hence, the environmental presence of these nanoparticles is growing progressively, enhancing the global concern on their potential health effects. Recent studies suggest that nanoceria may also have promising biomedical applications particularly in neurodegenerative and brain-re...
Toxicity of zinc oxide nanoparticles: Cellular and behavioural effects
Fernández-Bertólez, N; Alba-González, A; Touzani, A; Ramos-Pan, L; Méndez, J; Reis, AT; Quelle-Regaldie, A; Sánchez, L; Folgueira, M; Laffon, BDue to their extensive use, the release of zinc oxide nanoparticles (ZnO NP) into the environment is increasing and may lead to unintended risk to both human health and ecosystems. Access of ZnO NP to the brain has been demonstrated, so their potential toxicity on the nervous system is a matter of particular concern. Although evaluation of ZnO NP toxicity has been reported in several previous studies, the speci...
Association between cognitive reserve proxies and frailty phenotype: data from ...
Lorenzo-López, L; Cibeira, N; Hemadeh, A; López-López, R; Lema-Arranz, C; Maseda, A; Fernández-Bertólez, N; Costa, S; Pásaro, E; Valdiglesias, VA potential protective role of cognitive reserve proxies against frailty has been suggested in older adults. We explored the cross-sectional association between cognitive reserve indicators and frailty phenotype. Data were obtained from the UK Biobank. We included 31,975 dementia-free participants aged ≥ 60 years (50.7% females, 2.2% frail) who completed a web-based cognitive assessment (fluid intelligence, wor...
Toxoplasma gondii IgG Serointensity Is Positively Associated With Frailty
Mohyuddin, H; Laffon, B; Teixeira, JP; Costa, S; Teixeira-Gomes, A; Pásaro, E; Constantine, N; Dagdag, A; Ortmeyer, HK; Tizenberg, B; Afram, L; Yen, PYBackground: Persistent inflammation related to aging (inflammaging) is exacerbated by chronic infections and contributes to frailty in older adults. We hypothesized associations between Toxoplasma gondii (T. gondii), a common parasite causing an oligosymptomatic unremitting infection, and frailty, and secondarily between T. gondii and previously reported markers of immune activation in frailty.Methods: We analy...
Impact of gold nanoparticle exposure on genetic material
Ramos-Pan, L; Touzani, A; Fernández-Bertólez, N; Fraga, S; Laffon, B; Valdiglesias, VMetal nanoparticles, with gold nanoparticles (AuNP) at the forefront, have gained immense attention due to their unique properties. At the nanoscale, gold exhibits remarkable physical, chemical, optical, and electronic features, making it ideal for a plethora of applications, including bioimaging, sensing, diagnostics, and drug delivery. Despite their promising utility, concerns have arisen regarding the potent...
Assessing the in vitro toxicity of airborne (nano)particles to the human respir...
Bessa, MJ; Brandão, F; Rosário, F; Moreira, L; Reis, AT; Valdiglesias, V; Laffon, B; Fraga, S; Teixeira, JPSeveral studies have been conducted to address the potential adverse health risks attributed to exposure to nanoscale materials. While in vivo studies are fundamental for identifying the relationship between dose and occurrence of adverse effects, in vitro model systems provide important information regarding the mechanism(s) of action at the molecular level. With a special focus on exposure to inhaled (nano)pa...
Inter-laboratory variation in measurement of DNA damage by the alkaline comet a...
Moller, P; Azqueta, A; Collia, M; Bakuradze, T; Richling, E; Bankoglu, EE; Stopper, H; Bastos, VC; Langie, SAS; Jensen, A; Ristori, S; Scavone, FThe comet assay is a simple and versatile method for measurement of DNA damage in eukaryotic cells. More specifically, the assay detects DNA migration from agarose gel-embedded nucleoids, which depends on assay conditions and the level of DNA damage. Certain steps in the comet assay procedure have substantial impact on the magnitude of DNA migration (e.g. electric potential and time of electrophoresis). Inter-l...
DNA strand break levels in cryopreserved mononuclear blood cell lines measured ...
Moller, P; Azqueta, A; Rodriguez-Garraus, A; Bakuradze, T; Richling, E; Bankoglu, EE; Stopper, H; Bastos, VC; Langie, SAS; Jensen, A; Ristori, SThe comet assay is widely used in biomonitoring studies for the analysis of DNA damage in leukocytes and peripheral blood mononuclear cells. Rather than processing blood samples directly, it can be desirable to cryopreserve whole blood or isolated cells for later analysis by the comet assay. However, this creates concern about artificial accumulation of DNA damage during cryopreservation. In this study, 10 labo...
Effects of Zinc Oxide Nanoparticle Exposure on Human Glial Cells and Zebrafish ...
Valdiglesias, V; Alba-González, A; Fernández-Bertólez, N; Touzani, A; Ramos-Pan, L; Reis, AT; Moreda-Piñeiro, J; Yáñez, J; Laffon, B; Folgueira, MZinc oxide nanoparticles (ZnO NPs) are among the most widely used nanomaterials. They have multiple applications in cosmetics, textiles, paints, electronics and, recently, also in biomedicine. This extensive use of ZnO NPs notably increases the probability that both humans and wildlife are subjected to undesirable effects. Despite being among the most studied NPs from a toxicological point of view, much remains...
Long-term cryopreservation of potassium bromate positive assay controls for mea...
Moller, P; Azqueta, A; Rodriguez-Garraus, A; Bakuradze, T; Richling, E; Bankoglu, EE; Stopper, H; Bastos, VC; Langie, SAS; Jensen, A; Ristori, SThe formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay is widely used for the measurement of oxidatively generated damage to DNA. However, there has not been a recommended long-term positive control for this version of the comet assay. We have investigated potassium bromate as a positive control for the Fpg-modified comet assay because it generates many Fpg-sensitive sites with a little concurrent g...