2 documents found, page 1 of 1
Clinical and epidemiological profiles of patients with American cutaneous leish...
Melo,Maria Gabriella Nunes de; Morais,Rayana Carla Silva de; Goes,Tayná Correia de; Silva,Rômulo Pessoa e; Morais,Rômulo Freire deAbstract INTRODUCTION: Brazil has a high number of cases of American cutaneous leishmaniasis (ACL) in the north and northeast regions. Therefore, continuous surveillance of environmental and socioeconomic factors in endemic areas is needed to develop strategic control measures. This study aimed to describe the clinical and epidemiological profiles of patients with ACL. METHODS: All patients were from the states...
Tracking false-negative results in molecular diagnosis: proposal of a triplex-P...
Gonçalves-de-Albuquerque,Suênia da Cunha; Silva,Rômulo Pessoa e; Morais,Rayana Carla Silva de; Trajano-Silva,Lays Adrianne MendonçaBackground : Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasite’s DNA during extraction may significantly impair its detection by PCR and lead to false-negative results...