3 documents found, page 1 of 1
Skin in vitro models to study dermal white adipose tissue role in skin healing
Lago, M. E. L.; Cerqueira, M. T.; Pirraco, R. P.; Reis, R. L.; Marques, A. P.The perception of the role of adipose tissue has been changing over the years. For a long time, it was simply seen as an energy storage organ that confers mechanical protection and heat insulation. However, an increasing number of studies demonstrate the complex role of adipose tissue in the systemic regulation of processes such as immunity and inflammation. Therefore, adipose tissue is currently considered a m...
Natural melanin promotes the differentiation of an early epidermal cell fractio...
Silva, Lucília Pereira; Oliveira, Sílvia; Lago, M. E. L.; Cerqueira, Mariana Teixeira; Pirraco, Rogério P.; Santos, T. C.; Reis, R. L.; Correlo, V. M.The aim of the study was to determine whether melanin extracted from Sepia officinalis , common cuttlefish ink, affects the phenotype of an interfollicular keratinocyte cell fraction, and therefore propose a strategy to enhance wound re-epithelialization that also provides protection against ROS. To demonstrate the properties of the melanin, rapidly adherent human keratinocytes (KCs, passage<2, n=3) were expose...
Adipogenic cell sheets to recreate in vitro adipose tissue microenvironments
Lago, M. E. L.; Cerqueira, M. T.; Pirraco, Rogério P.; Costa, M.; Reis, R. L.; Marques, A. P.Cell sheet (CS) engineering, taking advantage of cellular self-matrix organized as in native tissue, has been largely explored, including by us, for different purposes [1â 3]. Herein we propose for the ï¬ rst time, the use of human adipose stem cells (hASCs)-derived CS to create adipose tissue analogues with different levels of maturation. hASCs were cultured on UpCellTM thermo-responsive dishes for 1, 3 and 5 ...