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Gene expression analysis identifies hypothetical genes that may be critical dur...
Laia, Marcelo Luiz de; Moreira, Leandro Marcio; Goncalves, Janaina Fernandes; Tiraboschi Ferro, Maria Ines [UNESP]; Pinto Rodrigues, Any CarolinyMade available in DSpace on 2020-12-10T19:41:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-11-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Fund for Citrus Plant Protection (FUNDECITRUS); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG); Coordenação de Aperfeiçoamento de Pessoal de Nível Super...
Gene expression analysis identifies hypothetical genes that may be critical dur...
Laia, Marcelo Luiz de; Moreira, Leandro Marcio; Gonçalves, Janaína Fernandes; Ferro, Maria Inês Tiraboschi; Rodrigues, Any Caroliny PintoBackground: Gene expression analysis via microarray is widely used in phytobacteria to validate differential gene expression associated with virulence or to compare biological profiles of wild type and mutant strains. Here, we employed DNA microarrays to study the early stages of the infection process (24, 72 and 120 h post-inoculation) of Xanthomonas citri subsp. citri (Xac) infecting Citrus sinensis to interr...
Clonagem e caracterização de análogos de genes de resistência e desenvolvimento...
Laia, Marcelo Luiz deCoordenação de Aperfeiçoamento de Pessoal de Nível Superior; A maioria dos genes de resistência (genes R) cIonados codifica proteínas que tém dominios conservados. Estas proteinas sao classificadas em classes ou subclasses de acordo com a presenca ou ausência desses domínios. Domínios do tipo LRR (Leucine rich repeat) e NBS (Nucleotide binding site) são os mais freqüentes em produtos dos genes R de mono e dicot...
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. ...
Ferreira, Rafael Marini; Moreira, Leandro Marcio; Ferro, Jesus Aparecido; Silva, Marcia Regina Soares da; Laia, Marcelo Luiz deCitrus canker is a major disease affecting citrus production in Brazil. It's mainly caused by Xanthomonas citri subsp. citri strain 306 pathotype A (Xac). We analysed the differential expression of proteins secreted by wild type Xac and an asymptomatic mutant for hrpB4 (1hrpB4) grown in Nutrient Broth (NB) and a medium mimicking growth conditions in the plant (XAM1). This allowed the identification of 55 secret...
Type IV secretion system is not involved in infection process in citrus
Jacob, Tiago Rinaldi [UNESP]; Laia, Marcelo Luiz de; Moreira, Leandro Marcio; Gonçalves, Janaína Fernandes; Carvalho, Flavia Maria de SouzaMade available in DSpace on 2015-12-07T15:30:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2014. Added 1 bitstream(s) on 2015-12-07T15:52:56Z : No. of bitstreams: 1 PMC3953393.pdf: 1404362 bytes, checksum: 7fd638962cfb366e4bee888c5c1efa00 (MD5); The type IV secretion system (T4SS) is used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope and into target cells. X...
Development and validation of a Xanthomonas axonopodis pv.citri DNA microarray ...
Moreira, Leandro Marcio; Laia, Marcelo Luiz de; Souza, Robson Francisco de; Zaini, Paulo Adriano; Silva, Ana C. R. da; Silva, Aline M. daBackground: From shotgun libraries used for the genomic sequencing of the phytopathogenic bacterium Xanthomonas axonopodis pv. citri (XAC), clones that were representative of the largest possible number of coding sequences (CDSs) were selected to create a DNA microarray platform on glass slides (XACarray). The creation of the XACarray allowed for the establishment of a tool that is capable of providing data for...
Type IV secretion system is not involved in infection process in citrus.
Jacob, Tiago Rinaldi; Laia, Marcelo Luiz de; Moreira, Leandro Marcio; Gonçalves, Janaína Fernandes; Carvalho, Flavia Maria de SouzaThe type IV secretion system (T4SS) is used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope and into target cells. Xanthomonas citri subsp. citri contains two copies of the T4SS, one in the chromosome and the other is plasmid-encoded. To understand the conditions that induce expression of the T4SS in Xcc, we analyzed, in vitro and in planta, the expression of 18 ORFs...
New genes of Xanthomonas citri subsp. citri involved in pathogenesis and adapta...
Laia, Marcelo Luiz de; Moreira, Leandro Marcio; Dezajacomo, Juliana; Brigati, Joice Bissoloti; Ferreira, Cristiano BarbalhoBackground: Citrus canker is a disease caused by the phytopathogens Xanthomonas citri subsp. citri, Xanthomonas fuscans subsp. aurantifolli and Xanthomonas alfalfae subsp. citrumelonis. The first of the three species, which causes citrus bacterial canker type A, is the most widely spread and severe, attacking all citrus species. In Brazil, this species is the most important, being found in practically all areas...
Novel insights into the genomic basis of citrus canker based on the genome sequ...
Moreira, Leandro Marcio; Almeida Junior, Nalvo Franco de; Potnis, Neha; Digiampietri, Luciano Antonio; Adi, Said Sadique; Bortolossi, Julio CesarBackground: Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. The three types have different phenotypes and affect different citrus species. The causative agent for type A is Xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. Xanthomonas fuscans subsp. aurantifolii strain B causes canke...