12 documents found, page 1 of 2
Serum amyloid A proteins reduce bone mass during mycobacterial infections
Gomes, AC; Sousa, DM; Oliveira, TC; Fonseca, O; Pinto, RJ; Silvério, D; Fernandes, AI; Moreira, AC; Silva, T; Teles, MJ; Pereira, L; Saraiva, MIntroductionOsteopenia has been associated to several inflammatory conditions, including mycobacterial infections. How mycobacteria cause bone loss remains elusive, but direct bone infection may not be required. MethodsGenetically engineered mice and morphometric, transcriptomic, and functional analyses were used. Additionally, inflammatory mediators and bone turnover markers were measured in the serum of healt...
Polymeric microspheres/cells/extracellular matrix constructs produced by auto-a...
Mielan, B; Sousa, DM; Krok-Borkowicz, M; Eloy, P; Dupont, C; Lamghari, M; Pamula, EModular tissue engineering (MTE) is a novel “bottom-up” approach to create engineered biological tissues from microscale repeating units. Our aim was to obtain microtissue constructs, based on polymer microspheres (MSs) populated with cells, which can be further assembled into larger tissue blocks and used in bone MTE. Poly(L-lactide-co-glycolide) MS of 165 ± 47 µm in diameter were produced by oil-in-water emul...
Sympathetic activity in breast cancer and metastasis: partners in crime
Conceição, F; Sousa, DM; Paredes, J; Lamghari, MThe vast majority of patients with advanced breast cancer present skeletal complications that severely compromise their quality of life. Breast cancer cells are characterized by a strong tropism to the bone niche. After engraftment and colonization of bone, breast cancer cells interact with native bone cells to hinder the normal bone remodeling process and establish an osteolytic “metastatic vicious cycle”. The...
Nociceptive mechanisms driving pain in a post-traumatic osteoarthritis mouse model
Alves, CJ; Couto, M; Sousa, DM; Magalhães, A; Neto, E; Leitão, L; Conceição, F; Monteiro, AC; Ribeiro-da-Silva, M; Lamghari, MIn osteoarthritis (OA), pain is the dominant clinical symptom, yet the therapeutic approaches remain inadequate. The knowledge of the nociceptive mechanisms in OA, which will allow to develop effective therapies for OA pain, is of utmost need. In this study, we investigated the nociceptive mechanisms involved in post-traumatic OA pain, using the destabilization of the medial meniscus (DMM) mouse model. Our resu...
Exploring Poly(Ethylene Glycol)-Poly(Trimethylene Carbonate) Nanoparticles as C...
Leite, DM; Sousa, DM; Lamghari, M; Pêgo, APCurrent treatment options for bone-related disorders rely on a systemic administration of therapeutic agents that possess low solubility and intracellular bioavailability, as well as a high pharmacokinetic variability, which in turn lead to major off-target side effects. Hence, there is an unmet need of developing drug delivery systems that can improve the clinical efficacy of such therapeutic agents. Nanoparti...
The Mechanisms Underlying the Biological Response to Wear Debris in Periprosthe...
Couto, M; Vasconcelos, DP; Sousa, DM; Sousa, B; Conceição, F; Neto, E; Lamghari, M; Alves, CJJoint replacement surgery is the gold-standard therapeutic approach to treat patients with end-stage hip and knee arthritis, providing pain relief and joint function recovery. Despite the improvements in implant design and surgical techniques, revisions after total joint replacement are expected to grow. The periprosthetic inflammation, featured by the sustained inflammatory response to the implant debris, elic...
Evaluation of nisin and ll-37 antimicrobial peptides as tool to preserve articu...
Najmi, Z; Kumar, A; Scalia, AC; Cochis, A; Obradovic, B; Grassi, FA; Leigheb, M; Lamghari, M; Loinaz, I; Gracia, R; Rimondini, LCartilage repair still represents a challenge for clinicians and only few effective therapies are nowadays available. In fact, surgery is limited by the tissue poor self-healing capacity while the autologous transplantation is often forsaken due to the poor in vitro expansion capacity of chondrocytes. Biomaterials science offers a unique alternative based on the replacement of the injured tissue with an artific...
Osteoblasts are inherently programmed to repel sensory innervation
Leitão, L; Neto, E; Conceição, F; Monteiro, AC; Couto, M; Alves, CJ; Sousa, DM; Lamghari, MTissue innervation is a complex process controlled by the expression profile of signaling molecules secreted by tissue-resident cells that dictate the growth and guidance of axons. Sensory innervation is part of the neuronal network of the bone tissue with a defined spatiotemporal occurrence during bone development. Yet, the current understanding of the mechanisms regulating the map of sensory innervation in th...
µSpikeHunter: An advanced computational tool for the analysis of neuronal commu...
Heiney, K; Mateus, JC; Lopes, CDF; Neto, E; Lamghari, M; Aguiar, PUnderstanding neuronal communication is fundamental in neuroscience, but there are few methodologies offering detailed analysis for well-controlled conditions. By interfacing microElectrode arrays with microFluidics (µEF devices), it is possible to compartmentalize neuronal cultures with a specified alignment of axons and microelectrodes. This setup allows the extracellular recording of spike propagation with a...
Interplay between sympathetic nervous system and inflammation in aseptic loosen...
Ribeiro da Silva, M.; Vasconcelos, DM; Alencastre, IS; Oliveira, MJ; Linhares, D; Neves, N; Costa, G; Henrique, R; Lamghari, M; Alves, CJInflammation is a common symptom in joint disorders such as rheumatoid arthritis, osteoarthritis (OA) and implant aseptic loosening (AL). The sympathetic nervous system is well known to play a critical role in regulating inflammatory conditions, and imbalanced sympathetic activity has been observed in rheumatoid arthritis. In AL it is not clear whether the sympathetic nervous system is altered. In this study we...