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Novel strategy to detect and locate periodontal pathogens: The PNA-FISH technique
Mendes, Luzia; Rocha, Rui; Azevedo, Andreia S.; Ferreira, Catarina; Henriques, Mariana; Pinto, Miguel Gonçalves; Azevedo, Nuno FilipePurpose: We aim to develop peptic nucleic acid (PNA) probes for the identification and localization of Aggregatibacter actinomycetemcomintans and Porphyromonas gingivalis in sub-gingival plaque and gingival biopsies by Fluorescence in situ Hybridization (FISH). Methods A PNA probe was designed for each microorganism. The PNA-FISH method was optimized to allow simultaneous hybridization of both microorganisms wi...
Date: 2016
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Origin: RepositóriUM - Universidade do Minho