34 documents found, page 1 of 4
Personalized bacteriophage therapy outcomes for 100 consecutive cases: a multic...
Pirnay, Jean-Paul; Djebara, Sarah; Steurs, Griet; Griselain, Johann; Cochez, Christel; De Soir, Steven; Glonti, Tea; Spiessens, An; Vanden Berghe, EmilyIn contrast to the many reports of successful real-world cases of personalized bacteriophage therapy (BT), randomized controlled trials of non-personalized bacteriophage products have not produced the expected results. Here we present the outcomes of a retrospective observational analysis of the first 100 consecutive cases of personalized BT of difficult-to-treat infections facilitated by a Belgian consortium i...
Exploring the transcriptional landscape of phagehost interactions using novel h...
Putzeys, Leena; Wicke, Laura; Brandão, Ana Catarina Ribeiro; Boon, Maarten; Pires, Diana Priscila Penso; Azeredo, Joana; Vogel, Jörg; Lavigne, RobIn the last decade, powerful high-throughput sequencing approaches have emerged to analyse microbial transcriptomes at a global scale. However, to date, applications of these approaches to microbial viruses such as phages remain scarce. Tailoring these techniques to virus-infected bacteria promises to obtain a detailed picture of the underexplored RNA biology and molecular processes during infection. In additio...
Impact of phage predation on P. aeruginosa adhered to human airway epithelium: ...
Brandão, Ana Catarina; Putzeys, Leena; Pires, Diana Priscila Penso; Voet, Marleen; Paeshuyse, Jan; Azeredo, Joana; Lavigne, RobPhage therapy is a promising adjunct therapeutic approach against bacterial multidrug-resistant infections, including Pseudomonas aeruginosa-derived infections. Nevertheless, the current knowledge about the phage-bacteria interaction within a human environment is limited. In this work, we performed a transcriptome analysis of phage-infected P. aeruginosa adhered to a human epithelium (Nuli-1 ATCC® CRL-4011). To...
CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipo...
Oliveira, Hugo Alexandre Mendes; Santos, Sílvio Roberto Branco; Pires, Diana Priscila Penso; Boeckaerts, Dimitri; Pinto, Graça; Domingues, RitaCitrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB\_CkoM\\_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i.e., all strains that were tested, but does not infect other species. Its linear 168,463-bp genome contains 291 coding sequences, sharing sequenc...
Impact of phage predation on bacterial transcriptome under simulated human airw...
Brandão, Ana Catarina Ribeiro; Putzeys, Leena; Pires, Diana Priscila Penso; Azeredo, Joana; Lavigne, RobBacteriophages have been proven to be efficient in the combat of bacterial multidrug-resistant infections, including those caused by Pseudomonas aeruginosa. Nevertheless, the interactions of phages with bacteria in the human body remains unexplained and its disclosure could lead to advance research and development in phage-based therapies. In this work, RNA-sequencing of phage-infected P. aeruginosa PAO1 adhere...
Isolation and characterization of the acadevirus members BigMira and MidiMira i...
da Silva, Jéssica Duarte; Bens, Lene; Santos, Adriele J. do Carmo; Lavigne, Rob; Soares, José; Melo, Luís Daniel Rodrigues; Vallino, MartaProteus mirabilis is an opportunistic pathogen and is responsible for more than 40% of all cases of catheter-associated urinary tract infections (CAUTIs). Healthcare-associated infections have been aggravated by the constant emergence of antibiotic-resistant bacterial strains. Because of this, the use of phages to combat bacterial infections gained renewed interest. In this study, we describe the biological and...
Viruses of Microbes: the latest conquests
Oliveira, Ana; São-José, Carlos; Pires, Diana Priscila Penso; Oliveira, Hugo Alexandre Mendes; Martins, Ivone M.; Azeredo, Joana; Dabrowska, KrystynaThis book covers abstracrs from the most recent advances in ecology and evolution of microbial viruses, virus structures and function, virus-host interaction, agro-food, veterinary and environmental biotechnology applications and phage therapy.
Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori
Ferreira, Rute; Sousa, Cláudia; Gonçalves, Raquel F.S.; Pinheiro, Ana Cristina; Oleastro, Mónica; Wagemans, Jeroen; Lavigne, Rob; Figueiredo, CeuHelicobacter pylori, a significant human gastric pathogen, has been demonstrating increased antibiotic resistance, causing difficulties in infection treatment. It is therefore important to develop alternatives or complementary approaches to antibiotics to tackle H. pylori infections, and (bacterio) phages have proven to be effective antibacterial agents. In this work, prophage isolation was attempted using H. p...
Characterization and genomic analysis of a new phage infecting Helicobacter pylori
Ferreira, Rute Vanessa Novais; Sousa, Cláudia Sofia Cunha; Gonçalves, Raquel; Pinheiro, A. C.; Oleastro, Mónica; Wagemans, Jeroen; Lavigne, RobHelicobacter pylori, a significant human gastric pathogen, has been demonstrating increased antibiotic resistance, causing difficulties in infection treatment. It is therefore important to develop alternatives or complementary approaches to antibiotics to tackle H. pylori infections, and (bacterio)phages have proven to be effective antibacterial agents. In this work, prophage isolation was attempted using H. py...
Differential transcription profiling of the phage LUZ19 infection process in di...
Brandão, Ana Catarina Ribeiro; Pires, Diana Priscila Penso; Coppens, Lucas; Voet, Marleen; Lavigne, Rob; Azeredo, JoanaRNA sequencing of phage-infected bacterial cultures offers a snapshot of transcriptional events occurring during the infection process, providing insights into the phage transcriptional organization as well as the bacterial response. To better mimic real environmental contexts, we performed RNA-seq of Pseudomonas aeruginosa PAO1 cultures infected with phage LUZ19 in a mammalian cell culture medium to better sim...