11 documents found, page 1 of 2
Engineered nascent living human tissues with unit programmability
Lavrador, Pedro; Moura, Beatriz S.; Almeida-Pinto, José; Gaspar, Vítor M.; Mano, João F.Leveraging human cells as materials precursors is a promising approach for fabricating living materials with tissue-like functionalities and cellular programmability. Here we describe a set of cellular units with metabolically engineered glycoproteins that allow cells to tether together to function as macrotissue building blocks and bioeffectors. The generated human living materials, termed as Cellgels, can be ...
Double‐interlinked colloidal gels for programable fabrication of supraparticle ...
Gonçalves, Leandro; Lavrador, Pedro; Amaral, Adérito J. R.; Ferreira, Luís P.; Gaspar, Vítor M.; Mano, João F.Engineering colloidal gel inks with suitable features for fabricating robust supraparticle architectures through 3D printing may overcome the challenges of precisely controlling nanoparticles spatial distribution across multiple scales. Herein, oppositely charged proteinaceous-polymeric nanoparticles are combined to generate multi-component colloidal gel (COGEL) inks for fabricating supraparticle volumetric arc...
Advancing tissue decellularized hydrogels for engineering human organoids
Moura, Beatriz S.; Monteiro, Maria V.; Ferreira, Luís P.; Lavrador, Pedro; Gaspar, Vítor M.; Mano, João F.The extracellular matrix plays a critical role in bioinstructing cellular self-assembly and spatial (re)configuration processes that culminate in human organoids in vitro generation and maturation. Considering the importance of the supporting matrix, herein we showcase the most recent advances in the bioengineering of decellularized tissue hydrogels for generating organoids and assembloids. Key design blueprint...
Double network laminarin-boronic/alginate dynamic bioink for 3D bioprinting cel...
Amaral, Adérito J. R.; Gaspar, Vítor M.; Lavrador, Pedro; Mano, João F.The design of dynamically crosslinked hydrogel bioinks for three-dimensional (3D) bioprinting is emerging as a valuable strategy to advance the fabrication of mechanically tuneable cell-laden constructs for 3Din vitrodisease modelling and tissue engineering applications. Herein, a dynamic bioink comprising boronic acid-functionalised laminarin and alginate is explored for bioprinting 3D constructs under physiol...
Engineering mammalian living materials towards clinically relevant therapeutics
Lavrador, Pedro; Gaspar, Vítor M.; Mano, João F.Engineered living materials represent a new generation of human-made biotherapeutics that are highly attractive for a myriad of medical applications. In essence, such cell-rich platforms provide encodable bioactivities with extended lifetimes and environmental multi-adaptability currently unattainable in conventional biomaterial platforms. Emerging cell bioengineering tools are herein discussed from the perspec...
Advanced bottom-up engineering of living architectures
Gaspar, Vítor M.; Lavrador, Pedro; Borges, João; Oliveira, Mariana B.; Mano, João F.Bottom-up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom-up assembly of unit...
Self-assembled bioactive colloidal gels as injectable multiparticle shedding pl...
Freitas, Bruno; Lavrador, Pedro; Almeida, Rui J.; Gaspar, Vítor M.; Mano, João F.Self-assembled colloidal gels are highly versatile 3D nanocluster platforms with potential to overcome the rapid clearing issues associated with standard free nanotherapeutics administration. However, the development of nanoassembled colloidal gels exhibiting autonomous multiparticle release from the bulk particle network remains elusive. Herein, we generated multiparticle colloidal gels from two nanosized buil...
Mechanochemical patternable ECM-Mimetic hydrogels for programmed cell orientation
Lavrador, Pedro; Gaspar, Vítor M.; Mano, João F.Native human tissues are supported by a viscoelastic extracellular matrix (ECM) that can adapt its intricate network to dynamic mechanical stimuli. To recapitulate the unique ECM biofunctionality, hydrogel design is shifting from typical covalent crosslinks toward covalently adaptable networks. To pursue such properties, herein hybrid polysaccharide-polypeptide networks are designed based on dynamic covalent as...
Bioinstructive Naringin-Loaded Micelles for Guiding Stem Cell Osteodifferentiation
Lavrador, Pedro; Gaspar, Vítor M; Mano, João FNaringin is a naturally occurring flavanone with recognized neuroprotective, cardioprotective, anti-inflammatory, and antiosteoporotic properties. Herein, the delivery of Naringin-loaded methoxy-poly(ethylene glycol)-maleimide-thiol-poly(l-lactide) (mPEGMSPLA) diblock polymeric micelles to human adipose-derived stem cells (hASCs) with the aim to augment its pro-osteogenic effect in these cells is reported for...
Bioinspired bone therapies using naringin: applications and advances
Lavrador, Pedro; Gaspar, Vítor M.; Mano, João FThe use of natural compounds for treating chronic bone diseases holds remarkable potential. Among these therapeutics, naringin, a flavanone glycoside, represents one of the most promising candidates owing to its multifaceted effect on bone tissues. This review provides an up-to-date overview on naringin applications in the treatment of bone disorders, such as osteoporosis and osteoarthritis, and further highlig...