2 documents found, page 1 of 1
Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for ...
Ahmed, Warish; Simpson, Stuart; Bertsch, Paul; Bibby, Kyle; Bivins, Aaron; Blackall, Linda; Bofill-Mas, Silvia; Bosch, Albert; Brandao, Joao; Choi, PhilWastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a comm...
Performance of Human Fecal Anaerobe-Associated PCR-Based Assays in a Multi-Labo...
Layton, Blythe; Yiping, Cao; Ebentier, Darcy; Kaitlyn, Hanley; Ballesté, Elisenda; Brandão, João; Byappanahalli, Muruleedhara; Converse, ReaganA number of PCR-based methods for detecting human fecal material in environmental waters have been developed over the past decade, but these methods have rarely received independent comparative testing in large multi-laboratory studies. Here, we evaluated ten of these methods (BacH, BacHum-UCD, B. thetaiotaomicron (BtH), BsteriF1, gyrB, HF183 endpoint, HF183 SYBR, HF183 Taqman, HumM2, and M. smithii nifH (Mnif)...