11 documents found, page 1 of 2
Ultrasound stimulation increases cartilage synthesis and revert dedifferentiati...
Oliveira, Sofia; Ferreira, Débora Carina Gonçalves Abreu; Padrão, Jorge; Catarino, Susana Oliveira; Monteiro, Francisca Andreia Azevedo; Zille, AndreaTherapeutic ultrasound (US) holds promise as a potential therapy to treat articular cartilage; however, optimized and targeted US protocols are lacking. This study aimed at determining an appropriate set of US protocols for stimulating human chondrocyte activity. Human chondrocytes were exposed to various US parameters, including different central frequencies, power densities, operation modes, pulse parameters,...
Experimental validation of time-explicit ultrasound propagation models with sou...
Fernandes, Nuno Alexandre Tavares Campos; Sharma, Shivam; Arieira, Ana Filipa Amorim; Hinckel, Betina; Silva, Filipe SamuelUltrasonic wave attenuation in biological tissues arises from complex interactions between mechanical, structural, and fluidic properties, making it essential to identify dominant mechanisms for accurate simulation and device design. This work introduces a novel integration of experimentally measured tissue parameters into time-explicit nonlinear acoustic wave simulations, in which the equations are directly so...
A Python FDTD method algorithm for 1D planar acoustic wave propagation: Simulat...
Fernandes, Nuno Alexandre Tavares Campos; Arieira, Ana Filipa Amorim; Hinckel, Betina; Silva, Filipe Samuel; Leal, Ana Isabel Neto CardosoNon-invasive techniques, such as high-frequency ultrasound, have emerged as promising therapeutic tools for neurological disorders, including Parkin-son’s disease and Alzheimer’s disease. By targeting specific brain regions, ultrasound stimulation modulates neural activity and induces beneficial physiological responses. However, simulating high-frequency acoustic wave propagation in biological tissues presents ...
Unlocking the secrets of knee joint unloading: a systematic review and biomecha...
Fernandes, Nuno Alexandre Tavares Campos; Arieira, Ana Filipa Amorim; Hinckel, Betina; Silva, Filipe Samuel; Carvalho, Óscar Samuel NovaisBackground/Objectives: This review analyzes the effects of invasive and non-invasive methods of knee joint unloading on knee loading, employing a biomechanical model to evaluate their impact. Methods: PubMed, Web of Science, Cochrane, and Scopus were searched up to 15 May 2024 to identify eligible clinical studies evaluating Joint Space Width, Cartilage Thickness, the Western Ontario and McMaster Universities O...
Development and validation of a multi-modal customized device to stimulate in v...
Oliveira, Sofia; Monteiro, Francisca Andreia Azevedo; Catarino, Susana Oliveira; Hinckel, Betina B.; Sotiropoulos, I.; Leal, Ana Isabel Neto CardosoThis work focuses on the development and validation of a multi-modal stimulation device for in vitro cell culture systems. The device was designed to stimulate cells or tissues placed on 12-well culture plates. It is connected to customized software that controls the parameters of photobiomodulation (PBM) and ultrasound stimulation (US) through light-emitting diodes and piezoelectric disks, respectively. A wide...
Biomechanical and clinical effects of spinal traction: enhancing lumbar health ...
Arieira, Ana Filipa Amorim; Fernandes, Nuno; Fernandes, Pedro; Silva, Filipe Samuel; Carvalho, Óscar Samuel Novais; Leal, Ana Isabel Neto Cardoso[Excerpt] Introduction: Lumbar disc degeneration (DD), one of the leading causes of disability worldwide, disrupts spinal biomechanics, leading to pain, disability, and reduced quality of life. Low back pain (LBP) affects up to 80% of the global population [1]. This degeneration process reduces disc height, alters load distribution, and contributes to herniation and nerve impingement [2]. Spinal traction, a non...
Micro and macromechanical modelling and simulation of bio elastomeric composite...
Fernandes, Nuno Alexandre Tavares Campos; Alves, Diana Isabel Sousa; Ferreira, Diana P.; Arieira, Ana Filipa Amorim; Silva, Filipe SamuelThis paper focuses on the virtual development of elastomeric composites incorporating natural fibers (Cordyline Australis) and an eco-friendly silicone matrix for medical phantoms that replicate the acoustic properties of human cranial skin. Micro- and macro-mechanical finite element models in COMSOL Multiphysics will be developed to simulate the anisotropic stress-strain behaviour of the composites under diffe...
Near-infrared photobiomodulation stimulates viability and cartilage matrix synt...
Oliveira, Sofia; Ferreira, Débora Carina Gonçalves Abreu; Padrão, Jorge; Catarino, Susana Oliveira; Monteiro, Francisca Andreia Azevedo; Zille, AndreaPhotobiomodulation (PBM) within red to near-infrared spectrum has been proposed as a method to restore articular cartilage injuries, due to its ability to modulate effectively chondrocyte activity, namely viability, proliferation and extracellular matrix synthesis. This in vitro study aimed to determine the most appropriate PBM dosage on human chondrocytes. Firstly, human chondrocytes were stimulated daily or e...
Optimization of therapeutic ultrasound stimulation to increase cells´ viability...
Oliveira, Sofia; Padrão, Jorge; Catarino, Susana Oliveira; Monteiro, Francisca Machado; Zille, Andrea; Silva, Filipe Samuel; Hinckel, Betina B.Objective: Therapeutic ultrasound (US) has been suggested to treat several human tissues due to its potential to promote cells´ activity. This study envisaged the establishment of an optimized US protocol to enhance cellular viability and proliferation. Methods: Human-derived cells were exposed daily or every other day to US for three days. Different central frequencies (0.45, 0.66, 1.00, 1.25, 1.50 and 2.00 MH...
Mechanical stimulation of human chondrocytes by ultrasound as a bioinspired str...
Oliveira, Sofia; Padrão, Jorge; Catarino, Susana Oliveira; Monteiro, Francisca Machado; Zille, Andrea; Silva, Filipe Samuel; Hinckel, Betina[Excerpt] Articular cartilage is naturally subjected to mechanical forces during knee joint loading. A constant and physiological loading of articular cartilage is essential for chondrocytes, the resident cells, that sense the mechanical deformations, activating their intracellular mechanisms to regulate and maintain the tissue homeostasis. However, either excessive or low magnitude mechanical loading may distu...