Made available in DSpace on 2022-04-29T08:45:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); The presence of fetal calf serum in culture medium influences embryo quality, causing a reduction in postcryopreservation survival. Forskolin has been used to induce lipolysis and increase cryotolerance, functioning as an activator of adenyl...
Made available in DSpace on 2022-04-29T08:45:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-06-01; This study aimed to examine the effects of nitric oxide (NO) and different phosphodiesterase (PDE) families on meiosis resumption, nucleotides levels and embryo production. Experiment I, COCs were matured in vitro with the NO donor S-nitroso-N-acetylpenicillamine (SNAP) associated or not with the solubl...
We evaluated the competence of bovine oocytes via morphological selection associated with follicular diameter and by brilliant cresyl blue (BCB) staining, in order to improve oocyte selection and the efficiency of in vitro embryo production. Follicles with small (2 < 4 mm), large (4–8 mm) or mixed (2–8 mm) diameters were aspirated from slaughterhouse ovaries. Next, the morphologically categorized oocytes wer...