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Prediction of sludge settleability, density and suspended solids of aerobic gra...

Leal, Cristiano; Val del Río, Angeles; Mesquita, D. P.; Amaral, António Luís Pereira; Ferreira, Eugénio C.

Steroid estrogens namely 17-estradiol (E2) and 17-ethinylestradiol (EE2) and antibiotics including sulfamethoxazole (SMX) are pharmaceutically active compounds (PhAC) of emerging concern due to their environmental and human health impacts even at ppb range concentrations. These compounds usually flow to wastewater treatment plants (WWTP) and are released to the aquatic systems due to inefficient removal in conv...


Assessment of an aerobic granular sludge system in the presence of pharmaceutic...

Leal, Cristiano; Lopes, Mariana; Val del Río, Angeles; Quintelas, Cristina; Castro, Paula M. L.; Ferreira, Eugénio C.; Amaral, A. Luís; Mesquita, D. P.

In this study, a sequencing batch reactor (SBR) with aerobic granular sludge (AGS) was operated with synthetic wastewater containing environmental relevant concentrations of 17-estradiol (E2), 17-ethinylestradiol (EE2) and sulfamethoxazole (SMX). Despite the presence of the studied PhAC, the granular fraction clearly predominated (TSSgran/TSS ranging from 0.82 to 0.98) throughout the monitoring period, presenti...


Environmental impact and biological removal processes of pharmaceutically activ...

Leal, Cristiano; Mesquita, D. P.; Amaral, A. Luís; Amaral, Almerinda M.; Ferreira, Eugénio C.

Pharmaceutically active compounds (PhACs) have recently received wide attention in the scientific community due to their extensive consumption for human health and consequent discharge to the environment. Release of PhACs into the environment, even in trace amounts, can cause serious environmental damage. This has become a major concern and their removal from water sources is a priority. Although a few PhACs ar...


Sludge volume index and suspended solids estimation of mature aerobic granular ...

Leal, Cristiano; Val, Angeles; Mesquita, D. P.; Amaral, A. Luís; Castro, Paula M. L.; Ferreira, Eugénio C.

Aerobic granular sludge (AGS) is considered a promising technology for wastewater treatment. Furthermore, it is recognized that the stability of the process is related to the balanced growth of the suspended (floccular) and granular fractions. Therefore, the development of adequate techniques to monitor this balance is of interest. In this work the sludge volume index (SVI), volatile suspended solids (VSS) and ...


Sludge volume index and suspended solids estimation of mature aerobic granular ...

Leal, Cristiano; Río, Angeles Val del; Mesquita, Daniela P.; Amaral, António L.; Castro, Paula M. L.; Ferreira, Eugénio C.

Aerobic granular sludge (AGS) is considered a promising technology for wastewater treatment. Furthermore, it is recognized that the stability of the process is related to the balanced growth of the suspended (floccular) and granular fractions. Therefore, the development of adequate techniques to monitor this balance is of interest. In this work the sludge volume index (SVI), volatile suspended solids (VSS) and ...


Density and sludge volume index estimation in mature aerobic granular sludge by...

Leal, Cristiano; Val, Angeles; Zlatkova, Anastasiya; Araújo, Bárbara; Mesquita, D. P.; Amaral, A. Luís; Ferreira, Eugénio C.

Quantitative image analysis (QIA) has already been applied for activated sludge dysfunctions and anaerobic granular sludge processes elucidation and its application to aerobic granular sludge characterization is considered of major interest. In this work, a sequencing batch reactor (SBR) was operated using mature aerobic granular sludge for pharmacologically active compounds (PAC) removal. The physicochemical p...


Use of chemometric analyses to assess biological wastewater treatment plants by...

Amaral, A. Luís; Leal, Cristiano; Vaz, A. Isabel; Vieira, J. Carvalho; Quinteiro, Andreia C.; Costa, M. Lourdes; Castro, L. Miguel

Protozoa and metazoa biota communities in biological wastewater treatment plants (WWTP) are known to be dependent of both the plant type (oxidation ditch, trickling filter, conventional activated sludge, among others) and the working operational conditions (incoming effluent characteristics, toxics presence, organic load, aeration, hydraulic and sludge retention times, nitrification occurrence, etc.). Thus, for...


Microbial evaluation of full-scale wastewater treatment plants by microscopy su...

Amaral, A. Luís; Leal, Cristiano

[Excerpt] Activated sludge (AS) systems, are constituted by living organisms, mainly bacteria (floc-forming and filamentous), protozoa and metazoa. The later play an important role on grazing bacteria, and are known to be dependent on the working operational parameters (incoming effluent, dissolved oxygen, nitrification, hydraulic and sludge retention times, transient phenomena, etc.) and the system itself (con...


Microbial-based evaluation of foaming events in full-scale wastewater treatment...

Leal, Cristiano; Amaral, A. L.; Costa, Maria de Lourdes

Activated sludge systems are prone to be affected by foaming occurrences causing the sludge to rise in the reactor and affecting the wastewater treatment plant (WWTP) performance. Nonetheless, there is currently a knowledge gap hindering the development of foaming events prediction tools that may be fulfilled by the quantitative monitoring of AS systems biota and sludge characteristics. As such, the present stu...


Biopolymer monitoring using quantitative image analysis techniques

Carvalheira, Mónica; Mesquita, D. P.; Leal, Cristiano; Oehmen, Adrian; Carvalho, Gilda; Cunha, J. R.; Amaral, A. L.; Ferreira, Eugénio C.

Polyhydroxyalkanoates (PHAs) are intracellular biopolymers with many applications, particularly as substitutes of polypropylene and polyethylene, due to their thermoplastic properties and biocompatible nature. Furthermore, glycogen is a polysaccharide of glucose with high importance in the metabolism of microbial communities and polyphosphate is a microbial storage compound that should be recovered in order to ...


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