7 documents found, page 1 of 1
Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture
Aguiar, Tatiana Quinta; Leal, Tânia; Rodrigues, Diana G.; Abrunhosa, Luís; Oliveira, Carla; Domingues, LucíliaEstablished chromatographic techniques for mycotoxin control in foodstuffs require prior sample enrichment and clean-up, typically achieved using immunoaffinity columns (IACs). Bovine serum albumin (BSA) has recently emerged as a cost-effective alternative to antibodies used in IACs. This study aimed at exploring the BSA domain II (BDII), which houses the primary binding site for ochratoxin A (OTA), as a biorec...
Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture
Aguiar, Tatiana Q.; Leal, Tânia; Rodrigues, Diana G.; Abrunhosa, Luís; Oliveira, Carla; Domingues, LucíliaEstablished chromatographic techniques for mycotoxin control in foodstuffs require prior sample enrichment and clean-up, typically achieved using immunoaffinity columns (IACs). Bovine serum albumin (BSA) has recently emerged as a cost-effective alternative to antibodies used in IACs. This study aimed at exploring the BSA domain II (BDII), which houses the primary binding site for ochratoxin A (OTA), as a biorec...
Carnosic acid production from sugarcane syrup by engineered yeast in fed-batch ...
Carsanba, Erdem; Fernandes, Sara; Beato, Felipe; Carvalho, Luís Carlos; Pintado, Ana; Lopes, Ana; Ribeiro, Mónica; Leal, Tânia; Pintado, ManuelaPhenolic diterpene carnosic acid (CA) is widely used in the food, nutritional health, and cosmetic industries due to its antioxidative and antimicrobial properties. This work aimed to overproduce CA in Saccharomyces cerevisiae from sugarcane syrup in fed-batch 2 L bioreactor fermentation. A geranylgeranyl diphosphate (GGPP)-producing strain modified with genes encoding the enzymes copalyl diphosphate synthase (...
Evaluation of ochratoxin a (OTA) interaction with recombinant domain II of bovi...
Aguiar, Tatiana Quinta; Leal, Tânia; Rodrigues, Diana Isabel Gomes; Abrunhosa, Luís; Oliveira, Carla; Domingues, LucíliaOchratoxin A (OTA) is a mycotoxin that raises food and feed safety concerns, existing legal limits worldwide for its presence in foodstuffs and beverages. Immuno-affinity columns (IACs) are typically used to capture, clean-up and pre-concentrate OTA from food samples before quantification by high- performance liquid chromatography, but their price limits their application. Serum albumins form stable complexes w...
Valorization of spent sugarcane fermentation broth as a source of phenolic comp...
Oliveira, Carla Maria; Horta, Bruno; Leal, Tânia; Pintado, Manuela; Oliveira, Catarina S. S.A methodology based on a solid phase extraction (SPE) was optimized for the recovery of phenolic compounds from the spent fermentation broth generated from Biofene® (trans-β-farnesene) production. For this purpose, two resins (XAD-2 and HP-20) and three desorption solutions (water, 50/50 ethanol/water, and ethanol) were tested. The most efficient resin revealed to be the HP-20, using ethanol as desorption solut...
BSA-based sample clean-up columns for ochratoxin A determination in wine: metho...
Leal, Tânia; Abrunhosa, Luís; Domingues, Lucília; Venâncio, Armando; Oliveira, CarlaAnalytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a n...
Boas práticas na gestão da expatriação: Um estudo de caso
Leal, TâniaEsta pesquisa pretende compreender as dinâmicas que prognosticam sucesso na adaptação dos expatriados com o fim de identificar as melhores práticas na gestão da expatriação. Neste sentido, recorreu-se ao estudo de caso de uma empresa portuguesa com experiência de expatriação de quadros para compreender o papel dos critérios de seleção, da formação e da família no estabelecimento do expatriado num novo destino. ...