31 documents found, page 1 of 4
Characterization of the cork oak transcriptome dynamics during acorn development
Miguel, Andreia; de Vega-Bartol, José; Marum, Liliana; Chaves, Inês; Santo, Tatiana; Leitão, J. M.; Varela, Maria C.; Miguel, Célia M.Background: Cork oak (Quercus suber L.) has a natural distribution across western Mediterranean regions and is a keystone forest tree species in these ecosystems. The fruiting phase is especially critical for its regeneration but the molecular mechanisms underlying the biochemical and physiological changes during cork oak acorn development are poorly understood. In this study, the transcriptome of the cork oak ...
A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...
Pereira-Leal, José B.; Abreu, Isabel A.; Alabaça, Cláudia S.; Almeida, Maria H.; Almeida, Paulo; Almeida, Tânia; Amorim, Maria I.; Araújo, SusanaBackground: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...
Correction of the misclassification of species in the Portuguese collection of ...
Rodrigues, R.; Veiga, I.; Marreiros, A.; Rocha, F.; Leitão, J. M.In this study, the genetic variability among 130 accessions of the Portuguese germplasm collection of Cucurbita pepo L. maintained at the Banco Portugues de Germoplasma Vegetal was assessed using AFLP (amplified fragment length polymorphism) and RAPD (random amplified polymorphic DNA) techniques for the identification of a genetically diverse core group of accessions for field phenotypic analysis. The surprisin...
The ENU-induced powdery mildew resistant mutant pea (Pisum sativum L.) lines S(...
Santo, Tatiana; Rashkova, M.; Alabaça, Cláudia S.; Leitão, J. M.Two pea (Pisum sativum L.) powdery mildew-resistant mutant lines, S(er1mut1) and F(er1mut2), were previously obtained by experimental chemical mutagenesis with ethylnitrosourea. Identification and subsequent analysis of the genomic sequence of the PsMLO1 gene revealed one single nucleotide mutation in each mutant line that leads to either a transversion or a transition, respectively, resulting in premature stop...
An integrated genetic map of pineapple (Ananas comosus (L.) Merr.)
de Sousa, N.; Carlier, Jorge; Santo, Tatiana; Leitão, J. M.The inclusion of multiple new markers, in particular 41 sequence specific markers, resulted in drastically improved version of a previously published F1-based genetic map of pineapple (Ananas comosus). The integration of the new version of this map with a more recently published F2-based map resulted in a map that assembles 741 loci: 739 DNA markers (25 SSR, 12 EST_SSR, 22 SCAR, 8 CAPS, 20 ISSR, 109 RAPD, and 5...
The downy mildew resistance locus Pp523 is located on chromosome C8 of Brassica...
Carlier, Jorge; Alabaça, Cláudia S.; Coelho, P. S.; Monteiro, A. A.; Leitão, J. M.We have previously constructed a genetic map of Brassica oleracea L. containing the Pp523 locus that confers downy mildew resistance to adult plants. In this work, 44 SSR markers of reference for the Brassica C genome chromosomes were added to the map, allowing the nine major linkage groups to be assigned to the nine chromosomes of B. oleracea. Locus Pp523 was located on chromosome C8, and a locus determining f...
Physical mapping in a triplicated genome: mapping the downy mildew resistance l...
Carlier, Jorge; Alabaça, Cláudia S.; Sousa, N. H.; Coelho, P. S.; Monteiro, A. A.; Paterson, A. H.; Leitão, J. M.We describe the construction of a BAC contig and identification of a minimal tiling path that encompass the dominant and monogenically inherited downy mildew resistance locus Pp523 of Brassica oleracea L. The selection of BAC clones for construction of the physical map was carried out by screening gridded BAC libraries with DNA overgo probes derived from both genetically mapped DNA markers flanking the locus of...
Factors affecting in vitro adventitious shoot formation on internode explants o...
Marques, N T.; Nolasco, Gustavo; Leitão, J. M.The in vitro formation of newly formed adventitious buds and shoots from internodal branch segments was studied on 12-month-old plants of Citrus aurantium L cv. Brazilian. The effects of 6-Benzyladenine (BA) and alpha-Naphthalene acetic acid (NAA) treatments were evaluated on adventitious bud and shoot regeneration. High rates of bud initiation and shoot development were obtained both with BA supplemented mediu...
Two powdery mildew resistance mutations induced by ENU in Pisum sativum L. affe...
Pereira, G.; Leitão, J. M.The alkylating compounds and strong mutagens methylnitrosourea (MNU) and ethylnitrosourea (ENU) were used to widen the genetic variability of pea (Pisum sativum L.) via experimental mutagenesis. Amongst multiple mutations of agronomical interest, for the first time two mutations conferring broad-range resistance to powdery mildew (Erysiphe pisi Syd.) were induced in pea. Mutagenic treatments were carried out on...
Identification of DNA markers linked to an induced mutated gene conferring resi...
Pereira, G.; Marques, C.; Ribeiro, R.; Formiga, S.; Damaso, M.; Sousa, M. T.; Farinho, M.; Leitão, J. M.We have recently induced two powdery mildew (Erysiphe pisi Syd) resistant mutants in Pisum sativum L. via ethylnitrosourea (ENU) mutagenesis. Both mutations (er1mut1 and er1mut2) affected the same locus er1 that determines most of the identified natural sources of powdery mildew resistance (PMR) in this crop. The mutated gene er1mut2 was mapped to a linkage group of 16 DNA markers combining three main strategie...