14 documents found, page 1 of 2

Is the efflux pump inhibitor Verapamil a potential booster for isoniazid agains...

The membrane-based efflux pump systems are recognized to have an important role in pathogenicity and drug resistance in Mycobacterium tuberculosis by the extrusion of toxic substrates and drugs from the inner bacillus. This study aimed to investigate the in vitro interaction of Verapamil (VP), an efflux pump inhibitor, with the classical first-line anti-tuberculosis drug isoniazid (INH) in resistant and suscept...

Anti-mycobacterium tuberculosis and cytotoxicity activities of Ruthenium(II)/ b...

The [Ru(Spym)(bipy)(P–P)]PF6, [Spym = pyrimidine-2-thiolate anion; P–P = 1,2-bis(diphenylphosphino)ethane, 1,3-bis(diphenylphosphino)propane and 1,1’-bis(diphenylphosphino)ferrocene] complexes were synthesized and characterized by spectroscopic, electrochemical and elemental analysis, and by X-ray crystallography. The minimal inhibitory concentration (MIC) of the compounds against Mycobacterium tuberculosis and...

Ruthenium(II) complexes with hydroxypyridinecarboxylates : screening potential ...

Three promising antimycobacterium tuberculosis ruthenium(II) complexes with the deprotonated ligands 2-hydroxynicotinic acid (2-OHnicH), 6-hydroxynicotinic acid (6-OHnicH) and 3-hydroxypicolinic acid (3-OHpicH) were synthesized and characterized. Structural analysis revealed three different coordination modes depending of the hydroxypyridinecarboxylate ligand. In the complex [Ru(2- OHnic)(dppb)(bipy)]PF6 (1), t...

Assessment of the quality of dna extracted by two techniques from Mycobacterium...

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:41:31Z No. of bitstreams: 1 S1517-83822011000200045.pdf: 1264755 bytes, checksum: 3bf7235dfcf1d3c501cc983c6b546de1 (MD5); Made available in DSpace on 2013-08-22T18:41:31Z (GMT). No. of bitstreams: 1 S1517-83822011000200045.pdf: 1264755 bytes, checksum: 3bf7235dfcf1d3c501cc983c6b546de1 (MD5) Previous issue date: 2011-06-01; Made availa...

Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis b...

Quantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis was used to screen for mutations related to drug resistance in Mycobacterium tuberculosis. We detected the C526T and C531T mutations in the rifampicin resistance-determining region (RRDR) of the rpoB gene with qPCR-HRM using plasmid-based controls. A segment of the RRDR region from M. tuberculosis H37Rv and from strains carrying ...

Phenolic compounds and antioxidant, antimicrobial and antimycobacterial activit...

Serjania erecta Radlk.(Sapindaceae) is a medicinal plant traditionally used in Brazil. We assayed the ethanolic extract of leaves and roots against seven microorganisms. The REMA (Resazurin Microtiter Assay) assay was used to measure the biological activity in vitro against Mycobacterium tuberculosis and our results showed moderate activity of the ethanolic extract. On the other hand S. aureus, P. aeruginosa, S...

Mycobacterium tuberculosis population structure shift in a 5-year molecular epi...

Starting with 257 outpatients attending the specialized health service for tuberculosis (TB) between 2002 and 2006 in Araraquara, an agro-industrial area with low tuberculosis (TB) incidence in São Paulo state, Brazil, positive mycobacterial cultures were obtained in 130 cases, of which 121 were confirmed as Mycobacterium tuberculosis complex. This report assesses the genetic diversity observed on 69.42% (n = 8...

Comparison of resazurin microtiter assay performance and BACTEC MGIT 960 in the...

Tuberculosis diagnosis after bleach processing for early stage tuberculosis lab...

The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resis...

Drug resistance in Mycobacterium tuberculosis clinical isolates from Brazil: ph...