8 documents found, page 1 of 1
Mycotoxins in Raw Bovine Milk: UHPLC-QTrap-MS/MS Method as a Biosafety Control ...
Leite, Marta; Freitas, Andreia; Barbosa, Jorge; Ramos, FernandoMycotoxins are compounds produced by several fungi that contaminate agricultural fields and, either directly or by carry-over, final food products. Animal exposure to these compounds through contaminated feed can lead to their excretion into milk, posing threats to public health. Currently, aflatoxin M1 is the sole mycotoxin with a maximum level set in milk by the European Union, as well as the most studied. No...
Regulated and Emerging Mycotoxins in Bulk Raw Milk: What Is the Human Risk?
Leite, Marta; Freitas, Andreia; Barbosa, Jorge; Ramos, FernandoMycotoxins are abiotic hazards whose contamination occurs at the pre- and post-harvest stages of the maize value chain, with animal exposure through contaminated feed leading to their excretion into milk. Currently, only aflatoxin M1 is regulated in milk products. Since feed materials and complete feed present a multi-mycotoxin composition and are the main mycotoxin source into milk, it is important to recogniz...
Comprehensive assessment of different extraction methodologies for optimization...
Leite, Marta; Freitas, Andreia; Barbosa, Jorge; Ramos, FernandoA multi-analyte method for identification and quantification of 23 regulated and emerging mycotoxins was devel- oped in maize samples by ultra high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Several extraction methodologies were evaluated, including solid-liquid extraction (SLE), solid- phase extraction (SPE), and modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged...
Mycotoxins and Maize Value Chain: Multi-Matrix and Multi-Analyte Tools towards ...
Leite, Marta; Freitas, Andreia; Barbosa, Jorge; Ramos, FernandoMaize value chains represent invaluable end-consumer products in food systems worldwide. Mycotoxin contamination along these chains causes severe economic and health impacts from the plant, animal and human safety points of view. This work aimed to develop a single standardized methodology that fulfilled extraction and detection procedures for 22 mycotoxins in maize chain matrices. The main goal concerned the v...
UHPLC-ToF-MS as a High-Resolution Mass Spectrometry Tool for Veterinary Drug Qu...
Leite, Marta; Marques, Ana Rita; Vila Pouca, Ana Sofia; Barros, Sílvia; Barbosa, Jorge; Ramos, Fernando; Afonso, Isabel Maria; Freitas, AndreiaMilk is one of the most widely consumed foods in the world, despite the increasing consumption of plant-based alternatives. Although rich in nutrients and believed by consumers to be free of undesirable contaminants, milk, whether of animal or plant origin, is not always free from residues of chemical substances, including veterinary medicines. For instance, in intensive livestock production, antibiotics are of...
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high pote...
Oliveira, A.; Leite, Marta; Kluskens, Leon; Santos, Sílvio Roberto Branco; Melo, Luís D. R.; Azeredo, JoanaEndolysins, which are peptidoglycan-degrading enzymes expressed during the terminal stage of the reproduction cycle of bacteriophages, have great potential to control Gram-pos- itive pathogens. This work describes the characterization of a novel endolysin (PlyPl23) encoded on the genome of Paenibacillus larvae phage phiIBB_Pl23 with high potential to control American foulbrood. This bacterial disease, caused by...
Correction to: The first Paenibacillus larvae bacteriophage endolysin (PlyPl23)...
Oliveira, A.; Leite, Marta; Kluskens, Leon; Santos, Sílvio Roberto Branco; Melo, Luís D. R.; Azeredo, JoanaThe Data Availability statement for this paper is incorrect. The correct statement is: All relevant data are within the paper and its Supporting Information files, except the aminoacidic sequence of the endolysin, which is available in RefSeq, accession number YP_008320357.1.
Development, optimization and application of an analytical methodology by ultra...
Leite, Marta; Freitas, Andreia; Azul, Anabela Marisa; Barbosa, Jorge; Costa, Saul; Ramos, FernandoAmanitins, highly toxic cyclopeptides isolated from various Amanita species, are the most potent poisons accounting for the hazardous effects on intestinal epithelium cells and hepatocytes, and probably the sole cause of fatal human poisoning. The present study was focused on the development, optimization and application of an analytical methodology by ultra performance liquid chromatography-tandem mass spectro...