5 documents found, page 1 of 1
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw...
Sgarioni,Sônia Aparecida; Hirata,Rosario Dominguez Crespo; Hiroyuki Hirata,Mario; Leite,Clarice Queico Fujimura; Prince,KarinaAndrade deMilk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk s...
Phenotypic and genotypic characterization of Rhodococcus equi isolated from sputum
Silva,Paulo da; Santos,Adolfo Carlos Barreto; Sato,Daisy Nakamura; Silva,Jaqueline Otero; Medeiros,Marta Inês Cazentini; Carneiro,Ana Maria MachadoINTRODUCTION: Rhodococcus equi is an opportunistic pathogen, causing rhodococcosis, a condition that can be confused with tuberculosis. Often, without identifying M. tuberculosis, physicians initiate empiric treatment for tuberculosis. R. equi and M. tuberculosis have different susceptibility to drugs. Identification of R. equi is based on a variety of phenotypic, chromatographic, and genotypic characteristics....
Inhibitors of human collagenase, MMP1
Leite,Sergio Roberto de AndradeDifferent common drugs (Meloxicam, Tenoxicam and Piroxicam, and sodium alendronate) were tested both experimental and theoretically as inhibitors of interstitial human collagenase, also known as matrix metalloproteinase 1 (MMP-1). The in vitro collagenase activity, alone and in the presence of inhibitors, was quantified by the reaction with a fluorescent synthetic substrate and measuring the change of emission....
Evaluation of anti-Mycobacterium tuberculosis activity of Campomanesia adamanti...
Pavan,Fernando Rogério; Leite,Clarice Queico Fujimura; Coelho,Roberta Gomes; Coutinho,Isabel Duarte; Honda,Neli Kika; Cardoso,Claudia Andréa LimaThe anti-Mycobacterium tuberculosis activity of Campomanesia adamantium fruits extracts were evaluated. Six compounds, identified as flavanones and chalcones were quantified by HPLC-DAD-UV. Promising antitubercular activity was observed with ethyl acetate extract (MIC 62.5 µg/mL) and their fractions (MIC values ranging from 39 to above 250 µg/mL). The better MIC result of 39 µg/mL was associated with two fracti...
Triterpenes and antitubercular activity of Byrsonima crassa
Higuchi,Celio Takashi; Pavan,Fernando Rogério; Leite,Clarice Queico Fujimura; Sannomiya,Miriam; Vilegas,Wagner; Leite,Sergio Roberto de AndradeWe evaluated the potential antitubercular activity of triterpenes obtained from leaves and bark of Byrsonima crassa. From chloroform extracts of the leaves, by bioassay-guided fractionation, we obtained mixtures of known triterpenes: α-amyrin, β-amyrin and their acetates, lupeol, oleanolic acid, ursolic acid and α-amyrinone. Tested against Mycobacterium tuberculosis, the triterpenes exhibited minimum inhibitory...