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Monitoring genetic diversity in tropical trees with multilocus dominant markers

Kremer, Antoine; Caron, Henri; Cavers, Stephen; Colpaert, Nathalie; Gheysen, Godelieve D.R.; Gribel, Rogério; Lemes, Maristerra R.; Lowe, Andrew J.

Since no universal codominant markers are currently available, dominant genetic markers, such as amplified fragment length polymorphism (AFLP), are valuable tools for assessing genetic diversity in tropical trees. However, the measurement of genetic diversity (H) with dominant markers depends on the frequency of null homozygotes (Q) and the fixation index (F) of populations. While Q can be estimated for AFLP lo...

Date: 2020   |   Origin: Oasisbr

Prey preference of the common vampire bat (Desmodus rotundus, Chiroptera) using...

Bobrowiec, Paulo Estefano Dineli; Lemes, Maristerra R.; Gribel, Rogério

Morphological identification of prey fragments in vampire bat feces is impossible because of an exclusively blood-based diet. Therefore, studies of their foraging ecology require innovative approaches. We investigated the diet of Desmodus rotundus using a PCR-restriction fragment length polymorphism (RFLP) molecular method by amplifying the cytochrome b mitochondrial gene (380 bp) from DNA fecal samples collect...

Date: 2020   |   Origin: Oasisbr

DNA Microsatellite Markers for Swartzia glazioviana (Fabaceae), a Threatened Sp...

Spoladore, Janaína; Mansano, Vidal F.; Freitas, Luan C Dias de; Magno Sebbenn, Alexandre; Lemes, Maristerra R.

Premise of the study: Development and characterization of a set of DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a naturally rare and threatened tree species, were carried out to investigate its conservation genetics. Methods and Results: Through an enriched genomic library procedure, 10 DNA microsatellite loci were isolated and characterized for the species. The mean expected heterozygosity w...

Date: 2020   |   Origin: Oasisbr

Standardized genetic diversity-life history correlates for improved genetic res...

Lowe, Andrew J.; Breed, Martin F.; Caron, Henri; Colpaert, Nathalie; Dick, Christopher W.; Finegan, Bryan; Gardner, Mike; Gheysen, Godelieve D.R.

Aim: Life history traits and range size are key correlates of genetic diversity in trees. We used a standardized sampling protocol to explore how life history traits and range size relate to the magnitude, variance and structuring (both between- and within-population) of genetic diversity in Neotropical tree species. Location: The Neotropics. Methods: We present a meta-analysis of new population genetic data ge...

Date: 2020   |   Origin: Oasisbr

Conservation implications of the mating system of the Pampa Hermosa landrace of...

Picanço-Rodrigues, Doriane; Astolfi-Filho, Spártaco A.T.; Lemes, Maristerra R.; Gribel, Rogério; Magno Sebbenn, Alexandre; Clement, Charles Roland

Peach palm (Bactris gasipaes) is cultivated by many indigenous and traditional communities from Amazonia to Central America for its edible fruits, and is currently important for its heart-of-palm. The objective of this study was to investigate the mating system of peach palm, as this is important for conservation and breeding. Eight microsatellite loci were used to genotype 24 open-pollinated progenies from thr...

Date: 2020   |   Origin: Oasisbr

Optimal sampling strategy for estimation of spatial genetic structure in tree p...

Cavers, Stephen; Degen, Bernd; Caron, Henri; Lemes, Maristerra R.; Margis, Rogério; Salgueiro, Fabiano; Lowe, Andrew J.

Fine-scale spatial genetic structure (SGS) in natural tree populations is largely a result of restricted pollen and seed dispersal. Understanding the link between limitations to dispersal in gene vectors and SGS is of key interest to biologists and the availability of highly variable molecular markers has facilitated fine-scale analysis of populations. However, estimation of SGS may depend strongly on the type ...

Date: 2020   |   Origin: Oasisbr

High genetic diversity among and within bitter manioc varieties cultivated in d...

Alves-Pereira, Alessandro; Peroni, Nivaldo; Cavallari, Marcelo Mattos; Lemes, Maristerra R.; Zucchi, Maria Imaculada; Clement, Charles Roland

Although manioc is well adapted to nutrient-poor Oxisols of Amazonia, ethnobotanical observations show that bitter manioc is also frequently cultivated in the highly fertile soils of the floodplains and Amazonian dark earths (ADE) along the middle Madeira River. Because different sets of varieties are grown in each soil type, and there are agronomic similarities between ADE and floodplain varieties, it was hypo...

Date: 2020   |   Origin: Oasisbr

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