10 documents found, page 1 of 1
Production of Human Milk Fat Substitutes by Lipase-Catalyzed Acidolysis: Immobi...
Soares, Cleide M. F.; Barbosa, Milson S.; Santos, Samuel B.; Mattedi, Silvana; Lima, Álvaro S.; Pereira, Matheus M.; Tecelão, CarlaHuman milk fat (HMF) triacylglycerols (TAGs) mainly contain palmitic acid esterified at the sn-2 position while oleic and other unsaturated fatty acids are located at positions sn-1,3. This study aimed at the production of HMF substitutes (HMFS) by lipase-catalyzed acidolysis of tripalmitin with oleic acid, in a solvent-free medium. Burkholderia cepacia lipase (BCL) was immobilized in silica (prepared with prot...
Production of human milk fat substitutes by lipase-catalyzed acidolysis: immobi...
Soares, Cleide M. F.; Barbosa, Milson S.; Santos, Samuel B.; Mattedi, Silvana; Lima, Álvaro S.; Pereira, Matheus M.; Tecelão, CarlaHuman milk fat (HMF) triacylglycerols (TAGs) mainly contain palmitic acid esterified at the sn-2 position while oleic and other unsaturated fatty acids are located at positions sn-1,3. This study aimed at the production of HMF substitutes (HMFS) by lipase-catalyzed acidolysis of tripalmitin with oleic acid, in a solvent-free medium. Burkholderia cepacia lipase (BCL) was immobilized in silica (prepared with prot...
Pre-treatment strategies based on aqueous two-phase systems comprising ionic li...
Pereira, Matheus M.; Brito, Larissa L. R.; de Miranda, Rita de C. M.; de Souza, Ranyere Lucena; Soares, Cleide M. F.; Coutinho, João A. P.ancer remains the cause of millions of deaths every year worldwide, and reliable early-stage diagnosis methods are essential to prevent them. Cancer biomarkers quantification in human fluids has gained relevance in detecting cancer in an early-stage. Among these, the levels of the biomarkers epinephrine, norepinephrine and vanillylmandelic acid in urine have been used for the diagnosis of adrenal cancer. Howeve...
Antimycotic nail polish based on humic acid-coated silver nanoparticles for ony...
Dantas, Ketheleen N. M.; Andrade, Lucas R.; Lisboa, Erika; Santana, Victoria L.; Dos Santos, André L. S.; Mello, Thaís P.; Sangenito, Leandro S.Background: The objective of this work has been the development of silver nanoparticles (AgNPs) coated with humic acid (HA) and their incorporation into an enamel for antifungal activity. AgNPs were synthesized by chemical reduction using sodium borohydride (NaBH4) as a reducing agent and coated with HA. Uncoated AgNPs were synthesized as control. AgNPs and HAAgNPs were characterized by ultravioletvisible spect...
Synthesis of dietetic structures lipids from spent coffee grounds crude oil cat...
Mota, Danyelle A.; Santos, Jefferson C.B.; Faria, Diana; Lima, Álvaro S.; Krauze, Laiza C.; Soares, Cleide M.F.; Ferreira-Dias, SuzanaThe aim of this study was the valorization of coffee industry residues, namely spent coffee grounds (SCG) as a source of oil, and silverskin (CS) as a source of both oil and biomass, under the concept of the circular economy. Therefore, crude oil from SCG was used to produce low-calorie structured lipids (SL) for food and pharmaceutical industries, and CS to produce biochar by pyrolysis for biotechnological use...
Increased significance of food wastes: selective recovery of added-value compounds
Reis, Igor A. O.; Santos, Samuel B.; Santos, Ludmila A.; Oliveira, Naiana; Freire, Mara G.; Pereira, Jorge F. B.; Ventura, Sónia P. M.A single-step selective separation of two food additives was investigated using alcohol-salt aqueous two-phase systems (ATPS). The selective partitioning of two of the most used additives from a processed food waste material, vanillin and l-ascorbic acid, was successfully accomplished. The results obtained prove that alcohol-salt ATPS can be easily applied as cheaper processes for the selective recovery of valu...
Ionic-liquid-based aqueous biphasic systems with controlled ph: the ionic liqui...
Ventura, Sónia P. M.; Sousa, Sílvia G.; Serafim, Luísa S.; Lima, Álvaro S.; Freire, Mara G.; Coutinho, João A. P.This work addresses the effect of different anions in imidazolium-based ionic liquids (ILs) through the formation of aqueous biphasic systems (ABS) using a phosphate buffer solution (KH 2PO 4/K 2HPO 4 mixture of salts) aiming at controlling the pH value of the coexisting aqueous phases. Twelve ABS were investigated and the corresponding solubility curves were determined by the cloud point titration method at 29...
Ionic liquid based aqueous biphasic systems with controlled pH: the ionic liqui...
Ventura, Sónia P. M.; Sousa, Sílvia G.; Serafim, Luísa S.; Lima, Álvaro S.; Freire, Mara G.; Coutinho, João A. P.This work addresses the evaluation of the ability of ionic liquid cations on the formation of aqueous biphasic systems (ABSʼs), with K₂HPO₄ or a mixture of inorganic salts, K₂HPO₄/KH₂PO₄, aiming at controlling the pH values of the coexisting aqueous phases. Using chloride-based ionic liquids, the effects of the cation core, the length of the alkyl side chain, and the positional isomerism on ABS formation abilit...
Design of ionic liquids for lipase purification
Ventura, Sónia P. M.; Sousa, Sílvia G.; Freire, Mara G.; Serafim, Luísa S.; Lima, Álvaro S.; Coutinho, João A. P.Aqueous two-phase systems (ATPS) are considered as efficient downstream processing techniques in the production and purification of enzymes, since they can be considered harmless to biomolecules due to their high water content and due to the possibility of maintaining a neutral pH value in the medium. A recent type of alternative ATPS is based on hydrophilic ionic liquids (ILs) and salting-out inducing salts. T...
Biodiesel Density: Experimental Measurements and Prediction Models
Pratas, Maria Jorge; Freitas, Samuel V. D.; Oliveira, Mariana B.; Monteiro, Silvia; Lima, Álvaro S.; Coutinho, João A. P.Density is an important biodiesel parameter, with impact on fuel quality. Predicting density is of high relevance for a correct formulation of an adequate blend of raw materials that optimize the cost of biodiesel fuel production while allowing the produced fuel to meet the required quality standards. The aim of this work is to present new density data for different biodiesels and use the reported data to evalu...