2 documents found, page 1 of 1

Sort by Issue Date
go first page go previous page
go to page: 1
go next page go last page
rss feed search results

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Ce...

Lima, AC; Jung, M; Rusch, J; Usmani, A; Lopes, AM; Conrad, DF

Fluorescence-activated cell sorting (FACS) has been one of the methods of choice to isolate enriched populations of mammalian testicular germ cells. Currently, it allows the discrimination of up to 9 murine germ cell populations with high yield and purity

Date: 2017   |   Origin: Repositório Aberto da Universidade do Porto
More info.

Multispecies Purification of Testicular Germ Cells

Lima, AC; Jung, M; Rusch, J; Usmani, A; Lopes, A; Conrad, DF

Advanced methods of cellular purification are required to apply genome technology to the study of spermatogenesis. One approach, based on flow cytometry of murine testicular cells stained with Hoechst-33342 (Ho-FACS), has been extensively optimized and currently allows the isolation of 9 germ cell types. This staining technique is straightforward to implement, highly effective at purifying specific germ cell ty...

Date: 2016   |   Origin: Repositório Aberto da Universidade do Porto
More info.
go first page go previous page
go to page: 1
go next page go last page
rss feed search results

2 Results

Queried text

Refine Results

Author








Date



Document Type


Access rights



Resource


Subject