4 documents found, page 1 of 1
Purification of a lectin from Cratylia mollis crude extract seed by a single st...
Nascimento, Cynthia Oliveira; Costa, Romero Marcos Pedrosa Brandão; Soares, Paulo Antônio; Herculano, Polyanna Nunes; Porto, Tatiana SouzaThe partitioning and purification of lectins from the crude extract of Cratylia mollis seeds (Cramoll 1,4) was investigated in aqueous two-phase systems (ATPS). A factorial design model (24) was used to evaluate the influence of polyethylene glycol (PEG) molar mass (15008000g/mol), PEG concentration (12.517.5% w/w), phosphate (1015% w/w) concentration, and pH (68) on the differential partitioning, purification ...
Collagenolytic enzymes produced by fungi: a systematic review
Wanderley, M.; Neto, José Manoel Werley Duarte; Filho, José Luiz de Lima; Lima, Carolina de Albuquerque; Teixeira, J. A.; Porto, Ana Lúcia FigueiredoSpecific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenas...
Purification and characterization of a collagenase from Penicillium sp. UCP 128...
Wanderley, M.; Neto, José Manoel Wanderley Duarte; Albuquerque, Wendell Wagner Campos; Marques, Daniela de Araújo Viana; Lima, Carolina de AlbuquerqueCollagenases are proteolytic enzymes capable of degrading both native and denatured collagen, reported to be applied in industrial, medical and biotechnological sectors. Liquid-liquid extraction using aqueous two-phase system (ATPS) is one of the most promising bioseparation techniques, which can substitute difficult solid-liquid separation processes, offering many advantages over conventional methods including...
Production and characterization of collagenase by Penicillium sp. UCP 1286 isol...
Wanderley, M.; Neto, José Manoel Wanderley Duarte; Lima, Carolina de Albuquerque; Silvério, Sara Isabel Cruz; Filho, José Luiz de Lima; Teixeira, J. A.A new Penicillium sp. strain isolated from the soil of Caatinga, a Brazilian Biome (UCP 1286) was selected for collagenase production. Fermentation system allowing obtention of collagenolytic activity about 2.7 times higher than existing data, with the highest values of collagenolytic and specific activity (379.80 U/mL, 1460.77 U/mg, respectively), after 126 hours. Applying a factorial design, enzyme production...