7 documents found, page 1 of 1
Exploiting the Integrated Valorization of Eucalyptus globulus Leaves: Chemical ...
Oliveira, Cátia S. D.; Moreira, Patrícia; Cruz, Maria T.; Pereira, Cláudia M. F.; Silva, Artur M. S.; Santos, Sónia A. O.; Silvestre, Armando J. D.E. globulus leaves have been mainly exploited for essential oil recovery or for energy generation in industrial pulp mills, neglecting the abundance of valuable families of extractives, namely, triterpenic acids, that might open new ways for the integrated valorization of this biomass. Therefore, this study highlights the lipophilic characterization of E. globulus leaves before and after hydrodistillation, aimi...
An integrated green process for the extraction of triterpenic acids fromEucalyp...
Oliveira, Cátia S. D.; Moreira, Patrícia; Cruz, Maria T.; Pereira, Cláudia M. F.; Gaspar, Alexandre; Neto, Carlos Pascoal; Pinto, Paula C. R. O.In this study the viability of the integrated exploitation of E. globulus leaf essential oil and triterpenic acids was evaluated through the development of a novel extraction process that can be implemented sequentially in a biorefinery context. Thus, essential oil (EO) collected by hydrodistillation was used for the first time as a bio-based solvent to recover triterpenic acids (TTAs) from the residue resultin...
Mitochondria Fusion upon SERCA Inhibition Prevents Activation of the NLRP3 Infl...
Pereira, Ana Catarina; Madeira, Nuno; Morais, Sofia; Macedo, António; Cruz, Maria Teresa; Pereira, Cláudia M. F.Sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) is a crucial component of the cellular machinery responsible for Ca2+ homeostasis. The selective inhibition of SERCA by thapsigargin (TG) leads to perturbations in Ca2+ signaling, which can trigger endoplasmic reticulum (ER) stress. The unfolded protein response (UPR) pathway is activated in response to ER stress and induces an adaptive response to preserve cell s...
Aβ and NMDAR activation cause mitochondrial dysfunction involving ER calcium re...
Ferreira, Ildete Luísa; Ferreiro, Elisabete; Schmidt, Jeannette; Cardoso, João M.; Pereira, Cláudia M. F.; Carvalho, Ana Luísa; Oliveira, Catarina R.
ER-mediated stress induces mitochondrial-dependent caspases activation in NT2 n...
Arduíno, Daniela M.; Esteves, A. Raquel; Domingues, A. Filipa; Pereira, Cláudia M. F.; Cardoso, Sandra M.; Oliveira, C. R.Recent studies have revealed that endoplasmic reticulum (ER) disturbance is involved in the pathophysiology of neurodegenerative disorders, contributing to the activation of the ER stress-mediated apoptotic pathway. Therefore, we investigated here the molecular mechanisms underlying the ER-mitochondria axis, focusing on calcium as a potential mediator of cell death signals. Using NT2 cells treated with brefeldi...
The release of calcium from the endoplasmic reticulum induced by amyloid-beta a...
Ferreiro, Elisabete; Oliveira, Catarina R.; Pereira, Cláudia M. F.In this study, we analyzed whether ER Ca2+ release, induced by amyloid-[beta] (A[beta]) and prion (PrP) peptides activates the mitochondrial-mediated apoptotic pathway. In cortical neurons, addition of the synthetic A[beta]1-40 or PrP106-126 peptides depletes ER Ca2+ content, leading to cytosolic Ca2+ overload. The Ca2+ released through ryanodine (RyR) and inositol 1,4,5-trisphosphate (IP3R) receptors was shown...
An endoplasmic-reticulum-specific apoptotic pathway is involved in prion and am...
Ferreiro, Elisabete; Resende, Rosa; Costa, Rui; Oliveira, Catarina R.; Pereira, Cláudia M. F.Prion (PrP) and amyloid-[beta] (A[beta]) peptides are involved in the neuronal loss that occurs in Prion disorders (PrD) and Alzheimer's disease (AD), respectively, partially due to Ca2+ dysregulation. Besides, the endoplasmic reticulum (ER) stress has an active role in the neurotoxic mechanisms that lead to these pathologies. Here, we analyzed whether the ER-mediated apoptotic pathway is involved in the toxic ...