16 documents found, page 1 of 2
Galectin-3 in prostate cancer and heart diseases: a biomarker for these two fri...
Lima, Tânia; Perpétuo, Luís; Henrique, Rui; Fardilha, Margarida; Leite-Moreira, Adelino; Bastos, Jose; Vitorino, RuiGalectin-3 (Gal-3) belongs to galectin protein family, a type of β-galactose-binding lectin having more than one evolutionarily conserved domain of carbohydrate recognition. Gal-3 is mainly located in the cytoplasm, but it also enters the nucleus and is secreted into the extracellular environment and biological fluids such as urine, saliva, and serum. It plays an important role in many biological functions, suc...
Gal-3 Protein Expression and Localization in Prostate Tumours
Lima, Tânia; Macedo-Silva, Catarina; Felizardo, Diana; Fraga, João; Carneiro, Isa; Jerónimo, Carmen; Henrique, Rui; Fardilha, Margarida; Vitorino, RuiGal-3 plays an important role in cell survival, mRNA splicing, and cell-cell and cell-matrix interactions. Depending on its cellular localization and cancer type, Gal-3 may have tumour-suppressive or tumour-promoting activities. Given the promising diagnostic role of Gal-3 in the urine of PCa patients found in our previous study, its concordant gene and protein expression levels, and its involvement in PCa-rela...
Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a sim...
António, Maria; Lima, Tânia; Ferreira, Rita; Fardilha, Margarida; Bastos, José Mesquita; Vitorino, Rui; Daniel-da-Silva, Ana L.Galectin-3 (Gal-3) is a carbohydrate-binding protein associated with the development and progress of heart failure. Here, we report the first colorimetric and low-cost approach for detecting and quantifying Gal-3 using gold nanoparticles (AuNPs) bioconjugated with Gal-3 antibody. The interaction of Gal-3 with the resulting nanoprobes led to a linear response of the absorbance ratio A750nm/A526nm to Gal-3 concen...
The World Café method and spaces dedicated to active teaching & learning: A dyn...
Pinto-Pinho, Patrícia; Ferreira, Ana; Matos, Bárbara; Santiago, Joana; Henriques, Magda Carvalho; Corda, Pedro Oliveira; Lima, Tânia; Rodrigues, MárioA pilot experiment was developed in a Biomedical Sciences curricular unit to test how using a different knowledge-sharing method (World Café) in a Space for Active Learning and Teaching would affect students’ engagement. Each working group was asked to prepare a poster summarising a different topic about the physiology of the oocyte. On the activity day, rotating discussion sessions moderated by tutors took pla...
Involvement of the Iron-Regulated Loci hts and fhuC in Biofilm Formation and Su...
Oliveira, Fernando; Lima, Tânia; Correia, Alexandra; Silva, Ana Margarida; Soares, Cristina; Morais, Simone; Weißelberg, Samira; Vilanova, ManuelStaphylococcus epidermidis is a major nosocomial pathogen with a remarkable ability to persist on indwelling medical devices through biofilm formation. Nevertheless, it remains intriguing how this process is efficiently achieved under the host’s harsh conditions, where the availability of nutrients, such as essential metals, is scarce. Following our previous identification of two iron-regulated loci putatively ...
mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence p...
Gaio, Vânia Silva; Lima, Tânia; Vilanova, Manuel; Cerca, Nuno; França, Ângela Maria Oliveira SousaStaphylococcus epidermidis biofilm cells are characterized by increased antimicrobial tolerance and improved ability to evade host immune system defenses. These features are, in part, due to the presence of viable but non-culturable (VBNC) cells. A previous study identified genes potentially involved in VBNC cells formation in S. epidermidis biofilms, among which SERP1682/1681 raised special interest due to the...
Involvement of the iron-regulated Loci hts and fhuCin biofilm formation and sur...
Oliveira, Fernando Eduardo Freitas; Lima, Tânia; Correia, Alexandra; Silva, Ana Margarida; Soares, Cristina; Morais, Simone; Weißelberg, SamiraStaphylococcus epidermidis is one of the most important nosocomial pathogens and a major cause of central line-associated bloodstream infections. Once in the bloodstream, this bacterium must surpass severe iron restriction in order to survive and establish infection. Staphylococcus epidermidis is a major nosocomial pathogen with a remarkable ability to persist on indwelling medical devices through biofilm forma...
Isolation and identification of an arabinogalactan extracted from pistachio ext...
Hamed, Mariem; Coelho, Elisabete; Bastos, Rita; Evtuguin, Dmitry V; Ferreira, Sónia S; Lima, Tânia; Vilanova, Manuel; Sila, Assaad; Coimbra, Manuel AThis work studies the extraction and purification of a novel arabinogalactan from pistachio external hull. It was extracted with a simple method from pistachio hull which is considered as unexploited waste. Based on the results of sugar analysis by GC-FID, glycosidic linkage by GC-MS, NMR spectroscopy, and molecular weight by Size Exclusion Chromatography, pistachio hull water soluble polysaccharides (PHWSP) we...
Integration of Automatic Text Mining and Genomic and Proteomic Analysis to Unra...
Lima, Tânia; Ferreira, Rita; Freitas, Marina; Henrique, Rui; Vitorino, Rui; Fardilha, MargaridaProstate cancer (PCa) is the most prevalent noncutaneous cancer among men. The limited accuracy and/or invasive nature of the current diagnostic tools have driven the demand for new and noninvasive biomarkers. Urine as a noninvasive sample that contains prostatic secretions is a promising source of PCa markers. The automatic text-mining functionality of VOSviewer was used to retrieve and create co-occurrence ne...
Application of Proteogenomics to Urine Analysis towards the Identification of N...
Lima, Tânia; Barros, António S; Trindade, Fábio; Ferreira, Rita; Leite-Moreira, Adelino; Barros-Silva, Daniela; Jerónimo, Carmen; Araújo, LuísTo identify new protein targets for PCa detection, first, a shotgun discovery experiment was performed to characterize the urinary proteome of PCa patients. This revealed 18 differentially abundant urinary proteins in PCa patients. Second, selected targets were clinically tested by immunoblot, and the soluble E-cadherin fragment was detected for the first time in the urine of PCa patients. Third, the proteogeno...