Intestinal parasites are among the most prevalent infections in humans. A cross-sectional survey was performed in a Canutama, Amazonas state, Brazil, in order to estimate the prevalence of intestinal parasites. The detection of parasites in the feces was performed using the Lutz method. Demographic data and the characteristics of the households were collected using a questionnaire. The prevalence of parasites w...
As espécies Duddingtonia flagrans e Monacrosporium thaumasium são microfungos considerados promissores agentes de controle biológico de parasitos. Sob condições adversas, como a falta de nutrientes, estes fungos produzem esporos capazes de sobreviverem após a passagem pelo trato gastrintestinal dos animais. A formação destas estruturas é uma característica desejável, já que promove a sobrevivência fúngica e a s...
-; With the purpose of investigating the biological aspects of Angiostrongylus vasorum (Baillet, 1866) Kamensky, 1905 in Subulina octona Bruguiere, 1789 experimental infections were carried out with the exposure of 90 adult molluscs to 155 L1 of the parasite/mollusc during 24 hours. The intramollus-can larval development was checked out daily through the chemical digestion of four infected molluscs and one moll...
A serine protease from the nematophagous fungus Monacrosporium thaumasium (NF34a) was purified, partially characterized and tested in vitro in control of the first larval stage of Angiostrongylus vasorum. NF34a grew in liquid culture medium, producing its crude extract that was purified by ion exchange chromatography. The fractions with high protease activity were collected in a pool, and elution of proteases w...
The dog acts as a reservoir and environmental disseminator of potentially zoonotic parasites. The objective of this work was to study the fungus Monacrosporium thaumasium regarding its nematicidal potential in laboratory trials and its proteolytic profile. The in vitro test was carried out through two assays (A and B). In assay A, conidia of the fungus N34a were added in positive coprocultures for Angiostrongyl...
The lymnaeids are important in the epidemiology of Fasciola hepatica, a neglected and endemic zoonosis. The interaction between the internal defense system of Pseudosuccinea columella and F. hepatica has been little studied. In the present study the effect of infection by F. hepatica on P. columella circulating haemocytes was investigated. Changes in the average number of total circulating haemocytes have been ...
Angiostrongylus vasorum is a nematode that parasitizes molluscs, dogs, and even man. The objective was to evaluate the predatory activity of the conidia of two fungal isolates of Duddingtonia flagrans (AC001 and CG722) on first-stage larvae (L1) of A. vasorum in laboratory conditions. At the end of the experiment, there were significant reductions (p<0.01) of 74.5% and 63.2%, on average, in the A. vasorum L1 re...
Angiostrongylus vasorum é um nematóide que parasita cães domésticos e eventualmente o homem. O objetivo deste trabalho foi observar a atividade predatória in vitro do extrato bruto enzimático do fungo Duddingtonia flagrans sobre larvas de primeiro estádio A. vasorum em condições laboratoriais no meio ágar-água 2%. Ao final do experimento, os percentuais de redução das L1 de A. vasorum observados foram de: 53,5%...
O objetivo deste trabalho foi otimizar a produção de proteases do fungo nematófago Monacrosporium thaumasium (NF34a), avaliar sua atividade larvicida e sua estabilidade biológica. Foi utilizado um isolado do fungo nematófago Monacrosporium thaumasium (NF34a) para a produção de enzima. O delineamento Plackett-Burman foi utilizado com o objetivo de se escanear quais componentes do meio de cultura, poderiam ter in...
O link do DOI está errado não pode ter escrito periódico capes.; The predatory nematophagous fungus Arthrobotrys sinensis (SF53) produces three proteases with nematicidal activity when grown on solid media culture. However, the proteolytic profile produced by this fungus, when grown in liquid culture medium remains unknown. Thus, the objective of this work was to evaluate the production of proteases from nemato...