6 documents found, page 1 of 1
Fibrinolytic protease production by new Streptomyces sp. DPUA 1576 from amazon ...
Silva, Germana Michelle de Medeiros e; Bezerra, Raquel P.; Teixeira, J. A.; Porto, Tatiana Souza; Lima-Filho, José. L.; Porto, Ana L. F.Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 22 factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentra...
Influence of trace elements supplementation on the production of recombinant fr...
Wanderley, Marcela Silvestre Outtes; Oliveira, Carla Cristina Marques de; Bruneska, Danyelly; Domingues, Lucília; Lima-Filho, José. L.; Teixeira, J. A.Frutalin, a galactose-specific lectin used to detect specific tumour markers, is a protein with low expression level in breadfruit. In the present study, fed-batch fermentation in a stirred tank bioreactor was used as a strategy to enhance protein production by a recombinant Pichia pastoris KM71H. By using this process, the production of recombinant frutalin was 4-fold higher than the value obtained in shaker f...
Influence of pH on cellular growth of Pichia pastoris KM71H by fed-batch process
Wanderley, Marcela Silvestre Outtes; Oliveira, Carla Cristina Marques de; Mussatto, Solange I.; Bruneska, Danyelly; Lima-Filho, José. L.Pichia pastoris is a methylotrophic yeast that can be genetically engineered to express proteins for industrial use. One of the most important advantages of protein expression in P. pastoris is its capability of growing on minimal medium and efficiently secreting heterologous proteins with low secretion levels of endogenous proteins. Operational variables such as pH, temperature, stirring rate, among others, us...
Identification of coalho cheese microbiot of districts of Pernambuco, Brazil
Neto, Torquato M. S.; Soares, Maria T. C. V.; Teixeira, J. A.; Domingues, Lucília; Lima-Filho, José. L.; Porto, Ana L. F.
MALDI-TOF mass spectrometry for identification of the Aspergillus niger aggregate
Santos, C.; Souza-Motta, Cristina Maria; Neves, R .P.; Araújo, J. M.; Calazans, G. M. T.; Carneiro-da-Cunha, Maria G.; Lima-Filho, José. L.; Erhard, M.
The microbial culture collections of the Federal University of Pernambuco (UFPE...
Motta, Cristina Souza; Neves, Rejane P.; Araújo, J. M.; Calazans, G. M. T.; Santos, Cledir; Carneiro-da-Cunha, Maria G.; Lima-Filho, José. L.The UFPE from Recife in Brazil hosts a bacterial (UFPEDA) and a fungal (URM) collections since 1951 and 1954, respectively. The UFPEDA was established by Prof. Oswaldo Gonçalves de Lima and is register in WDCM as 114. It is hosted at Antibiotic Department (DA) of UFPE and started out with 200 species mainly of the genus Streptomyces. Nowadays this collection holds 4000 strains of actinomycetes isolated from all...