4 documents found, page 1 of 1
All the pups we cannot see: Cannibalism masks perinatal death in laboratory mou...
Brajon, S; Morello, GM; Capas-Peneda, S; Hultgren, J; Gilbert, C; Olsson, IASPerinatal mortality is a major issue in laboratory mouse breeding. We compared a counting method using daily checks (DAILY_CHECK) with a method combining daily checks with detailed video analyses to detect cannibalisms (VIDEO_TRACK) for estimating the number of C57BL/6 pups that were born, that died and that were weaned in 193 litters from trios with (TRIOOVERLAP) or without (TRIO-NO_OVERLAP) the presence of an...
The discovery, distribution, and diversity of DNA viruses associated with Droso...
Wallace, MA; Coffman, KA; Gilbert, C; Ravindran, S; Albery, GF; Abbott, J; Argyridou, E; Bellosta, P; Betancourt, AJ; Colinet, H; Eric, KDrosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here, we report fourteen DNA viruses detected in a metagenomic analysis of 6668 pool-sequen...
Necropsy protocol for newborn mice
Capas-Peneda, S; Morello, GM; Lamas, S; Olsson, IAS; Gilbert, CNeonatal mortality is high in laboratory mouse breeding, and causes are poorly understood. Post-mortem analysis of pups is an often overlooked source of information and insight. We present a necropsy protocol for neonatal mice designed for easy practical application by animal technicians.
High laboratory mouse pre-weaning mortality associated with litter overlap, adv...
Morello, GM; Hultgren, J; Capas-Peneda, S; Wiltshire, M; Thomas, A; Wardle-Jones, H; Brajon, S; Gilbert, C; Olsson, IASHigh and variable pre-weaning mortality is a persistent problem in laboratory mouse breeding. Assuming a modest 15% mortality rate across mouse strains, means that approximately 1 million more pups are produced yearly in the EU to compensate for those which die. This paper presents the first large study under practical husbandry conditions to determine the risk factors associated with mouse pre-weaning mortalit...