8 documents found, page 1 of 1
The Centriole Stability Assay
Lince-Faria, Mariana; Silva, Ana; Pimenta-Marques, AnaFunding Information: This protocol was originally published in two research manuscripts by Pimenta-Marques et al. [17,18]. This work was supported by a grant from the Portuguese Research Council (FCT) awarded to APM (PTDC/BIA-BID/32225/2017). Publisher Copyright: © 2025 The Authors; exclusive licensee Bio-protocol LLC.; Centrosomes are vital eukaryotic organelles involved in regulating cell adhesion, polarity, ...
Ana1/CEP295 is an essential player in the centrosome maintenance program regula...
A, Pimenta-Marques; Perestrelo, Tania; Reis-Rodrigues, Patricia; Duarte, Paulo; Silva, Ana; Lince-Faria, Mariana; Bettencourt-Dias, MonicaCentrioles are part of centrosomes and cilia, which are microtubule organising centres (MTOC) with diverse functions. Despite their stability, centrioles can disappear during differentiation, such as in oocytes, but little is known about the regulation of their structural integrity. Our previous research revealed that the pericentriolar material (PCM) that surrounds centrioles and its recruiter, Polo kinase, ar...
From centrosomal microtubule anchoring and organization to basal body positioni...
Carmona, Bruno; Camelo, Carolina; Mehraz, Manon; Lemullois, Michel; Ferreira, David C.; Nolasco, Sofia; Lince-Faria, Mariana; Marino, H. SusanaCilia are microtubule-based organelles that protrude from the cell surface and fulfill critical motility and sensory functions being required for normal embryonic development and for homeostasis of human adult tissues. Cilia loss or dysfunction is associated with human ciliopathies. At their base cilia have a centriole/basal body (BB), which can be derived from the centrosome and assembles the ciliary axoneme. ...
TBCCD1: a new player in the development of ciliopathies?
Carmona, Bruno; Camelo, Carolina; Mehraz, Manon; Lemullois, Michel; Ferreira, David C.; Nolasco, Sofia; Lince-Faria, Mariana; Marinho, H. SusanaCilia are hair-like appendages, consisting of a microtubule (MT)-based ciliary axoneme, which fulfill critical motility and sensory functions required for normal embryonic development and also for homeostasis of adult tissues. At their base, cilia have a centriole/basal body, which can be derived from the centrosome, and that nucleates the ciliary axoneme. Centrosomes consist of a pair of centrioles surrounded ...
Centrosome positioning and development of ciliopathies: role of the human centr...
Carmona, Bruno; Camelo, Carolina; Mehraz, Manon; Lemullois, Michel; Ferreira, David C.; Nolasco, Sofia; Lince-Faria, Mariana; Marinho, H. SusanaAims/Context: Primary cilia are specialized microtubule-based signaling organelles that convey extracellular signaling and cellular polarity into a cellular response. Defects in primary cilia assembly/function cause severe diseases known as ciliopathies, typified by clinical manifestations, like infertility, obesity, brain problems, blindness, and kidney cysts. Primary cilia assembly entails centrosome migratio...
CDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesis
Zitouni, Sihem; Francia, Maria E.; Leal, Filipe; Montenegro Gouveia, Susana; Nabais, Catarina; Duarte, Paulo; Gilberto, Samuel; Brito, DanielaCentrioles are essential for the assembly of both centrosomes and cilia. Centriole biogenesis occurs once and only once per cell cycle and is temporally coordinated with cell-cycle progression, ensuring the formation of the right number of centrioles at the right time. The formation of new daughter centrioles is guided by a pre-existing, mother centriole. The proximity between mother and daughter centrioles was...
PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
Lopes, Carla A.M.; Jana, Swadhin Chandra; Cunha-Ferreira, Inês; Zitouni, Sihem; Bento, Inês; Duarte, Paulo; Gilberto, Samuel; Freixo, FranciscoCentrioles are essential for cilia and centrosome assembly. In centriole-containing cells, centrioles always form juxtaposed to pre-existing ones, motivating a century-old debate on centriole biogenesis control. Here, we show that trans-autoactivation of Polo-like kinase 4 (PLK4), the trigger of centriole biogenesis, is a critical event in the spatial control of that process. We demonstrate that centrioles prom...
Regulation of Autophosphorylation Controls PLK4 Self-Destruction and Centriole ...
Cunha-Ferreira, Inês; Bento, Inês; Pimenta-Marques, Ana; Jana, Swadhin Chandra; Lince-Faria, Mariana; Duarte, Paulo; Borrego-Pinto, JoanaPolo-like kinase 4 (PLK4) is a major player in centriole biogenesis: in its absence centrioles fail to form, while in excess leads to centriole amplification. The SCF-Slimb/βTrCP-E3 ubiquitin ligase controls PLK4 levels through recognition of a conserved phosphodegron. SCF-Slimb/βTrCP substrate binding and targeting for degradation is normally regulated by phosphorylation cascades, controlling complex processes...