4 documents found, page 1 of 1
Aptasensor for the detection of Moraxella catarrhalis adhesin UspA2
Sande, Maria; Ferreira, Débora; Rodrigues, Joana Lúcia Lima Correia; Melo, Luís Daniel Rodrigues; Saragliadis, Athanasios; Linke, DirkInnovative point-of-care (PoC) diagnostic platforms are desirable to surpass the deficiencies of conventional laboratory diagnostic methods for bacterial infections and to tackle the growing antimicrobial resistance crisis. In this study, a workflow was implemented, comprising the identification of new aptamers with high affinity for the ubiquitous surface protein A2 (UspA2) of the bacterial pathogen Moraxella ...
Design of new hydrolyzed collagen-modified magnetic nanoparticles to capture pa...
Sande, Maria Georgina; Roque, Lúcia; Braga, Adelaide Correia; Marques, Márcia; Ferreira, Débora Carina Gonçalves Abreu; Saragliadis, AthanasiosEnrichment and diagnosis tools for pathogens currently available are time consuming, thus the development of fast and highly sensitive alternatives is desirable. In this study, a novel approach was described that enables selective capture of bacteria expressing hydrolyzed collagen-binding adhesins with hydrolyzed collagen-coated magnetic nanoparticles (MNPs). This platform could be useful to shorten the time ne...
Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA o...
Sande, Maria Georgina; Ferreira, Débora; Rodrigues, Joana; Melo, Luís; Linke, Dirk; Silva, Carla J.; Moreira, Felismina; Sales, Goreti; Rodrigues, LígiaNew point-of-care (POC) diagnosis of bacterial infections are imperative to overcome the deficiencies of conventional methods, such as culture and molecular methods. In this study, we identified new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). Escherichia coli expressing YadA on ...
Electrochemical aptasensor for the detection of the key virulence factor YadA o...
Sande, Maria Georgina; Ferreira, Débora Carina Gonçalves Abreu; Rodrigues, Joana Lúcia Lima Correia; Melo, Luís Daniel Rodrigues; Linke, DirkNew point-of-care (POC) diagnosis of bacterial infections are imperative to overcome the deficiencies of conventional methods, such as culture and molecular methods. In this study, we identified new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). Escherichia coli expressing YadA on ...