11 documents found, page 1 of 2
Characterization and Vaccine Potential of Outer Membrane Vesicles from Photobac...
Teixeira, A; Loureiro, I; Lisboa, J; Oliveira, PN; Azevedo, JE; dos Santos, NMS; do Vale, APhotobacterium damselae subsp. piscicida (Phdp) is a Gram-negative fish pathogen with worldwide distribution and broad host specificity that causes heavy economic losses in aquaculture. Although Phdp was first identified more than 50 years ago, its pathogenicity mechanisms are not completely understood. In this work, we report that Phdp secretes large amounts of outer membrane vesicles (OMVs) when cultured in v...
Unconventional structure and mechanisms for membrane interaction and translocat...
Lisboa, J; Pereira, C; Pinto, RD; Rodrigues, IS; Pereira, LMG; Pinheiro, B; Oliveira, P; Pereira, PJB; Azevedo, JE; Durand, D; Benz, R; do Vale, ABacterial AB toxins are secreted key virulence factors that are internalized by target cells through receptor-mediated endocytosis, translocating their enzymatic domain to the cytosol from endosomes (short-trip) or the endoplasmic reticulum (long-trip). To accomplish this, bacterial AB toxins evolved a multidomain structure organized into either a single polypeptide chain or non-covalently associated polypeptid...
A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida...
Lisboa, J; Pereira, C; Rifflet, A; Ayala, J; Terceti, MS; Barca, AV; Rodrigues, IS; Pereira, PJB; Osorio, CR; Portillo, FGd; Boneca, IG; Vale, APeptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope. PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG. This work describes t...
Author Correction: Involvement of Hsp90 and cyclophilins in intoxication by AIP...
Rodrigues, IS; Pereira, LMG; Lisboa, J; Pereira, C; Oliveira, P; Santos, NMS; do Vale, A
Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals t...
Ithurbide, S; Coste, G; Lisboa, J; Eugénie, N; Bentchikou, E; Tour, CB; Lig, D; Confalonieri, F; Sommer, S; Quevillon-Cheruel, SC; Servant, PHorizontal gene transfer is a major driver of bacterial evolution and adaptation to environmental stresses, occurring notably via transformation of naturally competent organisms. The Deinococcus radiodurans bacterium, characterized by its extreme radioresistance, is also naturally competent. Here, we investigated the role of D. radiodurans players involved in different steps of natural transformation. First, we...
Role of AIP56 disulphide bond and its reduction by cytosolic redox systems for ...
Pereira, C; Rodrigues, IS; Pereira, LMG; Lisboa, J; Pinto, RD; Araújo, L; Oliveira, P; Benz, R; dos Santos, NMS; do Vale, AApoptosis-inducing protein of 56 kDa (AIP56) is a major virulence factor of Photobacterium damselae subsp. piscicida, a gram-negative pathogen that infects warm water fish species worldwide and causes serious economic losses in aquacultures. AIP56 is a single-chain AB toxin composed by two domains connected by an unstructured linker peptide flanked by two cysteine residues that form a disulphide bond. The A dom...
Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotea...
Rodrigues, IS; Pereira, LMG; Lisboa, J; Pereira, C; Oliveira, P; Santos, NMS; Vale, AAIP56 (apoptosis inducing protein of 56 kDa) is a key virulence factor secreted by virulent strains of Photobacterium damselae subsp. piscicida (Phdp), a Gram-negative bacterium that causes septicemic infections in several warm water marine fish species. AIP56 is systemically disseminated during infection and induces massive apoptosis of host macrophages and neutrophils, playing a decisive role in the disease o...
The RstAB system impacts virulence, motility, cell morphology, penicillin toler...
Terceti, M; Vences, A; Matanza, X; Barca, A; Noia, M; Lisboa, J; Santos, NMS; Vale, A; Osorio, CThe RstB histidine kinase of the two component system RstAB positively regulates the expression of damselysin (Dly), phobalysin P (PhlyP) and phobalysin C (PhlyC) cytotoxins in the fish and human pathogen Photobacterium damselae subsp. damselae, a marine bacterium of the family Vibrionaceae. However, the function of the predicted cognate response regulator RstA has not been studied so far, and the role of the R...
MouR controls the expression of the Listeria monocytogenes Agr system and media...
Pinheiro, J; Lisboa, J; Pombinho, R; Carvalho, F; Carreaux, A; Brito, C; Pöntinen, A; Korkeala, H; Santos, NM; Morais-Cabral, JH; Sousa, S; Cabanes, DThe foodborne pathogen Listeria monocytogenes (Lm) causes invasive infection in susceptible animals and humans. To survive and proliferate within hosts, this facultative intracellular pathogen tightly coordinates the expression of a complex regulatory network that controls the expression of virulence factors. Here, we identified and characterized MouR, a novel virulence regulator of Lm. Through RNA-seq transcri...
Neovascularização Coroideia de Causa Inflamatória: Opções Terapêuticas
Borges, B; Cabugueira, A; Domingues, I; Marques, M; Pinto Ferreira; Lisboa, J; Flores, RIntrodução: Uma complicação das doenças oculares inflamatórias é a neovascularização coroideia (NVC). Apesar de ser rara, é uma causa importante de perda de visão nestes doentes. As opções terapêuticas incluem, além do tratamento dirigido à doença inflamatória, a fotocoagulação a LASER, terapia fotodinâmina com verteporfina (TFD) e, mais recentemente, agentes farmacológicos anti-VEGF. Objectivo: Analisar uma sé...