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Subcellular distribution of some purine catabolism enzymes in brown trout (Salm...

Resende, Albina Dolores; Rocha, Eduardo; Lobo-da-Cunha, Alexandre

Background: Subcellular location of purine catabolism enzymes is of great interest in comparative studies due to the significant variability among species [1]. This metabolic pathway reflects in large extent a phylogenetic evolution with the loss of several enzymes in certain species, resulting in differences in the end products that are excreted [2]. Particularly in fishes, some of those enzymes are described ...

Date: 2024   |   Origin: Scientific Letters

High-quality draft genome sequence of Gaiella occulta isolated from a 150 meter...

Severino, Rita; Froufe, Hugo J.C.; Barroso, Cristina; Albuquerque, Luciana; Lobo-da-Cunha, Alexandre; Costa, Milton S. da; Egas, Conceição

Gaiella occulta strain F2-233T (=CECT 7815 = LMG 26412), isolated from a 150 meter deep mineral water aquifer, was deemed a candidate for high-quality draft genome sequencing because of the rare environment from which it was isolated. The draft genome sequence (QQZY00000000) of strain F2-233T is composed of approximately 3 Mb, predicted 3,119 protein-coding genes of which 2,545 were assigned putative functions....


Oryzisolibacter propanilivorax gen. nov., sp nov., a propanil-degrading bacterium

Vaz-Moreira, Ivone; Narciso-da-Rocha, Carlos; Lopes, Ana Rita; Carvalho, Gilda; Lobo-da-Cunha, Alexandre; Whitman, William B.; Snauwaert, Cindy

Strain EPL6T, a Gram-negative, motile, short rod was isolated from a propanil and 3,4-dichloroaniline enrichment culture produced from rice paddy soil. Based on the analyses of the 16S rRNA gene sequence, strain EPL6T was observed to be a member of the family Comamonadaceae , sharing the highest pairwise identity with type strains of the species Alicycliphilus denitrificans K601T (96.8 %) and Melaminivora alkal...


Hydromonas duriensis gen. nov., sp. nov., isolated from freshwater

Vaz-Moreira, Ivone; Narciso-da-Rocha, Carlos; De Brandt, Evie; Vandamme, Peter; Ferreira, A. C. Silva; Lobo-da-Cunha, Alexandre; Nunes, Olga C.

An aerobic, Gram-stain-negative rod, designated strain A2P5T , was isolated from the Douro river, in Porto, Portugal. Cells were catalase- and oxidase-positive. Growth occurred at15–30 8C, at pH 6–8 and in the presence of 1 % (w/v) NaCl. The major respiratory quinone was Q8, the genomic DNA had a G+C content of 47¡1 mol%, and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol were amongst...


Roseimaritima ulvae gen. nov., sp. nov. and Rubripirellula obstinata gen. nov.,...

Bondoso, Joana; Albuquerque, Luciana; Nobre, M. Fernanda; Lobo-da-Cunha, Alexandre; Costa, Milton S. da; Lage, Olga Maria

Four isolates, belonging to the deep-branching phylum Planctomycetes, were recovered from the biofilm of two marine macroalgae, Ulva sp. and Laminaria sp., from the Northern coast of Portugal. These strains were light pink- or red-pigmented; the cells were variable in shape and usually organized in rosettes. They had a dimorphic cell cycle with budding reproduction. The organisms were chemoheterotrophic, strict...


Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment...

Vaz-Moreira, Ivone; Figueira, Vânia; Lopes, Ana R.; Lobo-da-Cunha, Alexandre; Sproer, Cathrin; Schumann, Peter; Nunes, Olga C.; Manaia, Célia M.

A Gram-positive, aerobic, non-motile, endospore-forming rod, designated DS22T, was isolated from a drinking-water treatment plant. Cells were catalase- and oxidase-positive. Growth occurred at 15–37 6C, at pH 7–10 and with ,8% (w/v) NaCl (optimum growth: 30 6C, pH 7–8 and 1–3% NaCl). The major respiratory quinone was menaquinone 7, the G+C content of the genomic DNA was 36.5 mol% and the cell wall contained mes...


Extracellular matrix in Staphylococcus epidermidis biofilms: a consequence of b...

Cerca, Filipe; Andrade, Filipa; Correia, Alexandra; Teixeira, Luzia; Magalhães, Ângela; Hinzmann, Mariana; Ferreira, Íris; Ribeiro, Adília

Staphylococcus epidermidis is a leading pathogen accounting for nosocomial infections. The ability to form biofilms is considered the major virulence factor of this bacterium. The hallmark of this type of infection is the presence of an extracellular polymeric matrix that, in the case of S. epidermidis biofilms, is mainly constituted by an N-acetylglucosamine polymer. We have identified a subpopulation of bacte...


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