123 documents found, page 1 of 13
The role of liquid based cytology and ancillary techniques in the peritoneal wa...
Rossi, Esther; Bizzarro, Tommaso; Martini, Maurizio; Longatto Filho, Adhemar; Schmitt, Fernando; Fagotti, Anna; Scambia, Giovanni; Zannoni, Gian FrancoBackground The cytological analysis of peritoneal effusions serves as a diagnostic and prognostic aid for either primary or metastatic diseases. Among the different cytological preparations, liquid based cytology (LBC) represents a feasible and reliable method ensuring also the application of ancillary techniques (i.e immunocytochemistry-ICC and molecular testing). Methods We recorded 10348 LBC peritoneal effus...
Somatic mutations in solid tumors: a spectrum at the service of diagnostic arma...
Rossi, Esther Diana; Martini, Maurizio; Bizzarro, Tommazzo; Schmitt, Fernando; Longatto Filho, Adhemar; Larocca, Luigi MariaThis review article deals with the analysis and the detection of the morphological features associated with somatic mutations, mostly BRAF(V600E) mutation, on both cytological and histological samples of carcinomas. Few authors demonstrated that some architectural and specific cellular findings (i.e. polygonal eosinophilic cells defined as "plump cells" and sickle-shaped nuclei) are able to predict BRAF (V600E)...
The relation of HPV infection and expression of p53 and p16 proteins in esophag...
Pastrez, Paula Roberta Aguiar; Mariano, Vânia Sammartino; Costa, Allini Mafra da; Silva, Estela Maria; Scapulatempo-Neto, CristovamGOAL: To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma. MATERIALS AND METHODS: The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC). RESULTS: High-risk HPV (hr-HPV) wa...
Local and systemic immunomodulatory mechanisms triggered by Human Papillomaviru...
Alvarez, Karla Lucia Fernandez; Beldi, Mariana Carmezim; Sarmanho, Fabiane; Rossetti, Renata Ariza Marques; Silveira, Caio Raony FarinaCervical cancer is the last stage of a series of molecular and cellular alterations initiated with Human Papillomavirus (HPV) infection. The process involves immune responses and evasion mechanisms, which culminates with tolerance toward tumor antigens. Our objective was to understand local and systemic changes in the interactions between HPV associated cervical lesions and the immune system as lesions progress...
The Role of Self-Collection by Vaginal Lavage for the Detection of HPV and High...
Kuil, Larissa de Melo; Lorenzi, Adriana Tarla; Stein, Maíra Degiovani; Resende, Júlio César Possati; Antoniazzi, Márcio; Longatto Filho, AdhemarObjective: To compare the results of cervical cytology and high-risk HPV tests using samples obtained using two different collection modalities in a population of Brazilian women: self-collection (vaginal lavage) and cervical Pap testing. Methods: We enrolled 204 women who were aged 18-64 years and had previously obtained abnormal cervical cytology test results; 83.8% of them agreed to participate. The sample w...
High systemic IL-6 is associated with worse prognosis in patients with non-smal...
Silva, Estela Maria; Mariano, Vânia Sammartino; Pastrez, Paula Roberta Aguiar; Pinto, Miguel Cordoba; Castro, António G.; Syrjanen, Kari JuhaniCharacteristic cytokine patterns have been described in different cancer patients and they are related to their diagnosis, prognosis, prediction of treatment responses and survival. A panel of cytokines was evaluated in the plasma of non-small cell lung cancer (NSCLC) patients and healthy controls to investigate their profile and relationship with clinical characteristics and overall survival. The case-controll...
HPV infection and p53 and p16 expression in esophageal cancer: are they prognos...
Costa, Allini Mafra da; Fregnani, José Humberto Tavares Guerreiro; Pastrez, Paula Roberta Aguiar; Mariano, Vânia Sammartino; Silva, Estela MariaBackground: Esophageal squamous cell carcinoma (ESCC) is a highly lethal malignant tumor. Currently, Human papillomavirus (HPV) is suggested as a potential risk factor for esophageal cancer (EC) in addition to the classic risk factors, alcohol and tobacco, but this hypothesis still remains contradictory. We sought to investigate wether HPV and well-known biomarkers (p16 and p53) and patient-related factors that...
The metabolic microenvironment of melanomas: prognostic value of MCT1 and MCT4
Pinheiro, Céline; Baltazar, Maria de Fátima Monginho; Longatto Filho, Adhemar; Reis, R. M.BRAF mutations are known drivers of melanoma development and, recently, were also described as players in the Warburg effect, while this reprogramming of energy metabolism has been identified as a possible strategy for treating melanoma patients. Therefore, the aim of this work was to evaluate the expression and prognostic value of a panel of glycolytic metabolism-related proteins in a series of melanomas. The ...
Retrospective analysis of breast cancer prognosis among young and older women i...
Lima Vazquez, Fabiana de; Silva,Thiago Buosi; Vieira, René A. C.; Costa, Allini Mafra da; Scapulatempo-Neto, CristovamInvasive breast cancer (BC) is infrequent among women aged.5.40 years, however, the disease outlook in these younger patients is generally worse than among older women. The present study aimed to compare socio-demographic, clinical and pathological characteristics, and their association with long-term survival, between two random cohorts of young (<= 40 years) and older (50-69 years) Brazilian patients with BC....
Significance of glycolytic metabolism-related protein expression in colorectal ...
Martins, Sandra; Amorim, Ricardo Luís Agra de; Pereira, Marta Sofia Carvalho Ribeiro Viana; Pinheiro, Céline; Silva, Patrícia; Couto, Carla; Alves, SaraBackground: Colorectal cancer (CRC) is one of the most common malignancies and a leading cause of cancer death worldwide. Most cancer cells display high rates of glycolysis with production of lactic acid, which is then exported to the microenvironment by monocarboxylate transporters (MCTs). The main aim of this study was to evaluate the significance of MCT expression in a comprehensive series of primary CRC cas...