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Hunting for Answers: Assessing Brucella spp. Seroprevalence and Risks in Red De...

Pires, H; Cardoso, L; Lopes, AP; Fontes, MDC; Santos-Silva, S; Matos, M; Pintado, C; Roque, N; Fonseca, LF; Morgado, I; Dias, AS; Figueira, L; Matos, AC

Between 2016 and 2023, a cross-sectional study was conducted in the central region of Portugal in order to better understand the epidemiology and public health risks resulting from the handling and consumption of game animals infected with Brucella spp. The seroprevalence and risk factors for Brucella spp. seropositivity were evaluated. Antibodies against Brucella spp. were determined using a commercial enzyme-...


Molecular evidence of sporadic Coxiella burnetii excretion in sheep milk, centr...

Pires, H; Santos-Silva, S; Cruz, AVS; Cardoso, L; Lopes, AP; Pereira, MA; Nóbrega, C; Mega, AC; Santos, C; Cruz, R; Esteves, F; Vala, H; Matos, AC

Coxiella burnetii is the etiologic agent of Q fever, a worldwide zoonosis. Cattle, sheep and goats are considered the main reservoirs of the disease. Transmission to humans occurs mainly through the inhalation of infectious aerosols from milk, faeces, urine, and birth products from infected ruminants. In this study, a 2-year longitudinal approach was performed to ascertain the excretion of C. burnetii in bulk t...


Serological evidence of antibodies to Flaviviridae in wild birds in Portugal

Loureiro, F; Cardoso, L; Matos, AC; Pintado, C; Silva, F; Ferreira, M; Brandão, R; Lopes, C; Lopes, AP; Mesquita, JR; Matos, M; Coelho, AC

Emerging infectious diseases are a major threat to biodiversity and an important public health issue. Flaviviruses are the cause of several emerging vector-borne zoonotic arboviruses whose distribution is currently increasing in Europe. The evidence that West Nile virus (WNV) circulates in resident and migratory species has implications for both animal and public health and should therefore be studied in depth....


Expert Perspectives on the Management of Alpha 1-Antitrypsin Deficiency

Conde, B; Costa, F; Gomes, J; Lopes, AP; Mineiro, A; Rodrigues, O; Santos, C; Semedo, L; Sucena, M; Guimarães, C

Alpha 1-antitrypsin deficiency is an inherited autosomal codominant disorder, which predisposes patients to lung and/or liver disease. Even though it is considered rare, it is one of the most frequent genetic disorders worldwide, albeit remaining underdiagnosed. Several organizations and societies, including the Portuguese Society of Pulmonology have been elaborating guidelines and recommendations for the diagn...


Morphologic and Genetic Analysis of Synhimantus (Synhimantus) laticeps from a L...

Lopes, C; Santos-Silva, S; Nunes, C; Mendes, S; Costa, C; Brazio, E; Coutinho, T; Rodrigues, FT; Mesquita, JR; Coelho, AC; Cardoso, L; Lopes, AP

The long-eared owl (Asio otus) is a medium-sized owl species that is well-distributed in almost all of the territories in Portugal. Nematodes were found in the oral cavity of a long-eared owl (A. otus) admitted to CRASSA (Wildlife Rehabilitation Centre of Santo Andre). During a physical exam and stabilization of the bird, five nematodes were collected. The worms were examined and measured under light microscopy...


Seropositivity for Coxiella burnetii in Wild Boar (Sus scrofa) and Red Deer (Ce...

Pires, H; Cardoso, L; Lopes, AP; Fontes, MD; Matos, M; Pintado, C; Figueira, L; Mesquita, JR; Matos, AC; Coelho, AC

Q fever is caused by the pathogen Coxiella burnetii and is a zoonosis that naturally infects goats, sheep, and cats, but can also infect humans, birds, reptiles, or arthropods. A survey was conducted for the detection of antibodies against C. burnetii in a sample of 617 free-ranging wild ruminants, 358 wild boar (Sus scrofa) and 259 red deer (Cervus elaphus), in east-central Portugal during the 2016-2022 huntin...


Prevalence and Risk Factors for Hepatitis E Virus in Wild Boar and Red Deer in ...

Pires, H; Cardoso, L; Lopes, AP; Fontes, MDC; Santos-Silva, S; Matos, M; Pintado, C; Figueira, L; Matos, AC; Mesquita, JR; Coelho, AC

Hepatitis E virus (HEV) is a zoonotic foodborne virus with an annual infection prevalence of 20 million human cases, which seriously affects public health and economic development in both developed and developing countries. To better understand the epidemiology of HEV in Central Portugal, a cross-sectional study was conducted from 2016 to 2023 with sera samples from wild ungulates. The seroprevalence and risk f...


First report of human Thelazia callipaeda infection in Portugal

Morgado, ACT; do Vale, B; Ribeiro, P; Coutinho, T; Santos-Silva, S; de Sousa Moreira, A; Rodrigues, FT; Coelho, AC; Lopes, AP; Mesquita, JR; Cardoso, L

The zoonotic parasitic nematode Thelazia callipaeda, also known as the oriental eye worm, is endemic in several European countries, including Portugal. Infections may result in ocular disease in domestic and wild animals as well as humans, with more or less severe manifestations. We report the first human case of ocular thelaziosis by T. callipaeda in Portugal, a country where the parasite had already been foun...


Outdoor Cultivation of the Microalga Chlorella vulgaris in a New Photobioreacto...

Lopes, AP; Santos, FM; Silva, TFCV; Vitor Vilar; Pires, JCM

Microalgae can be a future source of biomass with a wide range of applications, including its use to solve current environmental issues. One of the main variables for microalgal cultivation is the light supply: (i) its intensity that often does not present a uniform spatial distribution inside the culture; (ii) photoperiod; and (iii) spectrum. Therefore, this study aims to evaluate the growth of the microalgae ...


Serological and molecular evidence of Bartonella henselae in cats from Luanda c...

Barradas, PF; de Sousa, R; Vilhena, H; Oliveira, AC; Luz, MF; Granada, S; Cardoso, L; Lopes, AP; Gonçalves, H; Mesquita, JR; Ferreira, P; Amorim, I

A total of 100 domestic cats from Luanda (Angola) were tested for the presence of antibodies against Bartonella henselae and spotted fever group of Rickettsia (SFGR) using indirect immunofluorescence assay (IFA). Molecular screening targeting the riboflavin synthase (ribC) gene for Bartonella and outer membrane protein B (ompB) gene for Rickettsia, using conventional PCR and sequencing was also performed in cat...


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