72 documents found, page 1 of 8
Loss of antiphospholipid antibody positivity decreases the risk of recurrent th...
Gaspar, Pedro; Cruz-Machado, Ana Rita; Abrantes, Ana Mafalda; Costa, Filipa; Parreira, Inês; Lopes, Ana Rita; Costa-Silva, Ryan; Chícharo, Ana TeodósioOBJECTIVE: To establish the predictive value of antiphospholipid antibody (aPL) negativization on recurrent thrombosis in thrombotic antiphospholipid syndrome (APS). METHODS: Retrospective cohort study including all consecutive adult patients with APS followed at Hospital de Santa Maria, Lisbon, Portugal, up to December 2024. At diagnosis, all patients were positive for solid-phase aPL. Then, patients were cate...
Frequency, characteristics and predictors oc central and peripheral neurologica...
Costa, R. Pereira da; Bandeira, Matilde; Silvério-António, Manuel; Lopes, Ana Rita; Santos, Filipe Cunha; Pereira, Paulo J.; Raimundo, Diana BelchiorBackground: Sjogren's disease (SjD) systemic affection is being increasingly recognized. The prevalence of peripheral (PNS) and central (CNS) nervous system involvement in SjD and risk factors for these manifestations have not been clearly established. Objectives: To define the clinical characteristics of SjD patients with CNS and/or PNS involvement and to find predictors of this involvement.
PORTRESS – the PORTuguese Reuma.pt registry for Sjögren’S disease
Bandeira, Matilde; Silvério-António, Manuel; Costa, Roberto Pereira da; Lopes, Ana Rita; Santos, Filipe Cunha; Pereira, Paulo J.Aims: Sjögren's disease (SjD) is a complex disease with a wide variety of manifestations and outcomes. We recently created PORTRESS, the Portuguese SjD registry within Reuma.pt. We aim to describe this registry and characterize our national cohort. Methods: We included patients with a clinical diagnosis of SjD, registered in PORTRESS up to November 2023. Demographic, clinical, treatment, and patient-reported ou...
Genomic DNA extraction from honey bee (Apis mellifera) queen spermathecal content
Yadró, Carlos A.; Lopes, Ana Rita; Henriques, Dora; Musin, Eduard; Wegener, Jakob; Pinto, M. AliceGenetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing...
Kinetics and isotherm study of ceftriaxone removal using functionalized biochar...
Cruz-Lopes, Luísa; Araújo, Rodrigo; Lopes, Ana Rita; Moles, Samuel; Romero-Sarria, Francisca; Esteves, BrunoThe increasing presence of antibiotics such as cephalosporins in wastewater represents a significant environmental risk. These compounds are excreted in large quantities, and conventional wastewater treatment plants are often ineffective at their removal. Consequently, the development of more sustainable and efficient treatment technologies is essential. In this study, the removal of cephalosporins from aqueous...
Large Scale Mitochondrial Evidence of C-lineage Dominance in European Honey Bees
Li, Fernanda; Lopes, Ana Rita; Costa, Maíra; Henriques, Dora; Quaresma, Andreia; Yadró Garcia, Carlos A.; Albo, Alexandre; Čereškienė, Laima BlažytėIn Europe, distribution of the several endemic honey bee (Apis mellifera) subspecies has suffered a considerable shift in the last century. In particular, beekeepers tend to favour subspecies of Eastern European ancestry (C-lineage), such as the Italian honey bee (A. m. ligustica), due to their perceived docility and high honey production. As a result, large scale migratory beekeeping and trade of C-lineage que...
Development of LAMP Primers for the Detection of Pyrethroid Resistance Mutation...
Costa, Maíra; Yadró Garcia, Carlos A.; Lopes, Ana Rita; Bejaoui, Mohamed Khalil; Almeida, Jhennifer; Correia, Lucas; Sánchez, Sara; Li, FernandaVarroa destructor is one of the main threats to Apis mellifera L., directly affecting colony health and contributing to their global decline. Control of this mite is traditionally achieved using acaricides, with pyrethroids such as tau-fuvalinate and fumethrin being the most used, acting on voltage-gated sodium channels (VGSC). However, the intensive use of these compounds by beekeepers has led to the emergence...
Genetic variation of detoxification genes: from genes to proteins
Henriques, Dora; Li, Fernanda; Bashir, Sana; Quaresma, Andreia; Lopes, Ana Rita; Taliadoros, Demetris; Webster, Matthew; Shiraishi, Carlos S.H.Honey bees (Apis mellifera) are exposed to natural and synthetic xenobiotics, requiring genetic adaptations for survival. Several gene families have been implicated in insect pesticide resistance, including cytochrome P450s, glutathione-S-transferases (GSTs), esterases, and uridine diphosphate (UDP)-glycosyltransferases. This study investigates genetic variation in these detoxification gene families and predict...
Evolution of amitraz resistance in varroa destructor: historical assessment of ...
Costa, Maíra; Pérez-Pérez, António; Santos, Ana Clara; Prado, Rafaela; Lopes, Doralinda; Lopes, Ana Rita; Yadró Garcia, Carlos A.Varroa destructor is one of the main parasites of the honey bee (Apis mellifera), causing significant colony losses worldwide. The control of this mite often relies on acaricides, with amitraz being among the most widely used products in Portugal and Spain over the last decade. However, the continuous and persistent use of the same active substance exerts strong selective pressure on parasite populations, poten...
An unparalleled survey of honey bee genetic diversity in Europe – insights from...
Pinto, M. Alice; Li, Fernanda; Lopes, Ana Rita; Taliadoros, Demetris; Costa, Maíra; Yadró Garcia, Carlos A.; Cunha, Larissa; Henriques, DoraEurope is home to 10 subspecies of Apis mellifera, classified into four main lineages sensu Ruttner: M (western and northern European), C (southeastern European), A (African), and O (Middle Eastern). However, large-scale movements of honey bee genetic material—driven by transhumance and, more importantly, queen trading—has intensified gene flow, particularly from the C-lineage subspecies A. m. carnica and A. m....