54 documents found, page 1 of 6
Genomic DNA extraction from honey bee (Apis mellifera) queen spermathecal content
Yadró, Carlos A.; Lopes, Ana Rita; Henriques, Dora; Musin, Eduard; Wegener, Jakob; Pinto, M. AliceGenetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing...
Pregnancy outcomes in systemic sclerosis: experience of a rheumatology-obstetri...
Chícharo, Ana Teodósio; Lopes, Ana Rita; Barreira, Sofia; Martins, Patrícia; Machado, Ana Rita Cruz; Pinto, Luísa; Capela, SusanaSystemic sclerosis (SSc) is a rare multisystemic connective tissue disease (CTD). It is mainly characterized by progressive fibrosis and non-inflammatory vasculopathy. Women diagnosed with SSc seem to be at increased risk of developing adverse pregnancy outcomes (APO), but maternal and perinatal outcomes remain poorly understood in these patients, with no data yet available from Portuguese centers.
Kinetics and isotherm study of ceftriaxone removal using functionalized biochar...
Cruz-Lopes, Luísa; Araújo, Rodrigo; Lopes, Ana Rita; Moles, Samuel; Romero-Sarria, Francisca; Esteves, BrunoThe increasing presence of antibiotics such as cephalosporins in wastewater represents a significant environmental risk. These compounds are excreted in large quantities, and conventional wastewater treatment plants are often ineffective at their removal. Consequently, the development of more sustainable and efficient treatment technologies is essential. In this study, the removal of cephalosporins from aqueous...
Origins, diversity, and adaptive evolution of DWV in the honey bees of the Azor...
Lopes, Ana Rita; Low, Matthew; Martín-Hernández, Raquel; Pinto, M. Alice; Miranda, Joachim R. deDeformed wing virus (DWV) is a honey bee virus, whose emergence from relative obscurity is driven by the recent host-switch, adaptation, and global dispersal of the ectoparasitic mite Varroa destructor (a highly efficient vector of DWV) to reproduction on honey bees (Apis mellifera). Our study examines how varroa affects the continuing evolution of DWV, using the Azores archipelago, where varroa is present on o...
Varroa destructor shapes the unique viral landscapes of the honey bee populatio...
Lopes, Ana Rita; Low, Matthew; Martín-Hernández, Raquel; Miranda, Joachim R. de; Pinto, M. AliceThe worldwide dispersal of the ectoparasitic mite Varroa destructor from its Asian origins has fundamentally transformed the relationship of the honey bee (Apis mellifera) with several of its viruses, via changes in transmission and/or host immunosuppression. The extent to which honey bee-virus relationships change after Varroa invasion is poorly understood for most viruses, in part because there are few places...
An unprecedented large-scale survey of honey bee mitochondrial diversity in Eur...
Li, Fernanda; Costa, Maíra; Lopes, Ana Rita; Gonçalves, Telma; Henriques, Dora; Quaresma, Andreia; Yadró Garcia, Carlos A.; Albo, AlexandreEurope is home to ten Apis mellifera subspecies, which belong to three mitochondrial lineages: the Western European (M), Eastern European (C), and African (A). However, the long-standing human-mediated movement of queens, primarily of C-lineage ancestry, has threatened the genetic integrity of many of these native subspecies through introgression and replacement. This has led to the establishment of conservatio...
Projeto MITE- Varroa e vírus transmitidos: monItorização de muTações e dEsenvol...
Henriques, Dora; Yadró Garcia, Carlos A.; Lopes, Ana Rita; Rufino, José; Costa, Maíra; Coelho, Marta; Martín-Hernández, Raquel; Higes, MarianoA abelha melífera (Apis melífera L.) tem sido ameaçada por diferentes fatores, tais como pesticidas, alterações climáticas, má nutrição, espécies invasoras tais como o predador Vespa velutina, a bactéria causadora da loque americana Paenibacillus larvae, e principalmente, o ácaro parasita Varroa destructor (vulgarmente conhecido como varroa), que é apontado como umas das mais importantes ameaças para a abelha m...
Early life stage mechanisms of an active fish species to cope with ocean warmin...
Lima, André R.A.; Booms, Emily M.; Lopes, Ana Rita; Martins-Cardoso, Sara; Novais, Sara C; Lemos, Marco F.L.; Ribeiro, Laura; Castanho, SaraOcean’s characteristics are rapidly changing, modifying environmental suitability for early life stages of fish. We assessed whether the chronic effects of warming (24 ◦C) and hypoxia (<2–2.5 mg L− 1 ) will be amplified by the combination of these stressors on mortality, growth, behaviour, metabolism and oxidative stress of early stages of the white seabream Diplodus sargus. Combined warming and hypoxia synergi...
Integrated behavioural and physiological responses of sand smelt larvae to the ...
Lima, André R.A.; Lopes, Ana Rita; Martins-Cardoso, Sara; Moutinho, Ariana B.; Lemos, Marco F.L.; Novais, Sara C; Faria, Ana M.Forecasts indicate that rising temperatures towards the future and the expansion of dead zones will change environmental suitability for fish early stages. Therefore, we assessed the chronic effects of warming (26 ◦C), hypoxia (<2–2.5 mg L− 1 ) or their combination on mortality rate, growth, behaviour, energy metabolism and oxidative stress using Atherina presbyter larvae as a model species. There were no diffe...
Dual challenge inside the womb: a case report of concomitant fetal atrio-ventri...
Chícharo, Ana Teodósio; Rebelo, Mónica; Lopes, Ana Rita; Saavedra, Maria João; Paramés, Maria Filipa; Araújo, Ana Rita; Machado, Ana Rita CruzFetal autoimmune atrioventricular block (AVB) is a rare but potentially life-threatening condition. It results from the passage of maternal anti-SSA/Ro or Anti SSB/La auto-antibodies into the fetal circulation, leading to inflammation and fibrosis of the AV node and often to irreversible damage. Besides AVB, these antibodies can also cause cardiomyopathies, but there is no evidence linking them to tachyarrhythm...