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The membrane periodic skeleton is an actomyosin network that regulates axonal d...
Costa, AR; Sousa, SC; Pinto-Costa, R; Mateus, JC; Lopes, CDF; Costa, AC; Rosa, D; Machado, D; Pajuelo, L; Wang, X; Zhou, FQ; Pereira, AJ; Sampaio, PNeurons have a membrane periodic skeleton (MPS) composed of actin rings interconnected by spectrin. Here, combining chemical and genetic gain-and loss-of-function assays, we show that in rat hippocampal neurons the MPS is an actomyosin network that controls axonal expansion and contraction. Using super-resolution microscopy, we analyzed the localization of axonal non-muscle myosin II (NMII). We show that active...
µSpikeHunter: An advanced computational tool for the analysis of neuronal commu...
Heiney, K; Mateus, JC; Lopes, CDF; Neto, E; Lamghari, M; Aguiar, PUnderstanding neuronal communication is fundamental in neuroscience, but there are few methodologies offering detailed analysis for well-controlled conditions. By interfacing microElectrode arrays with microFluidics (µEF devices), it is possible to compartmentalize neuronal cultures with a specified alignment of axons and microelectrodes. This setup allows the extracellular recording of spike propagation with a...
BDNF gene delivery mediated by neuron-targeted nanoparticles is neuroprotective...
Lopes, CDF; Gonçalves, NP; Gomes, CP; Saraiva, MJ; Pêgo, APNeuron-targeted gene delivery is a promising strategy to treat peripheral neuropathies. Here we propose the use of polymeric nanoparticles based on thiolated trimethyl chitosan (TMCSH) to mediate targeted gene delivery to peripheral neurons upon a peripheral and minimally invasive intramuscular administration. Nanoparticles were grafted with the non-toxic carboxylic fragment of the tetanus neurotoxin (HC) to al...