3 documents found, page 1 of 1
Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryop...
Wanderleya, Livia Schell; Luza, Hiédely Kenia Machado; Faustino, Luciana Rocha; Lima, Isadora Machado Teixeira; Lopes, Cláudio Afonso PinhoThe objective was to develop an efficient protocol for cryopreservation of agouti (Dasyprocta aguti) ovarian tissue. Agouti ovarian fragments were placed, for 10 min, in a solution containing MEM and fetal bovine serum plus 1.5 M dimethyl sulfoxide (DMSO), ethylene glycol (EG) or propanediol (PROH); some of those fragments were subsequently cryopreserved in a programmable freezer. After exposure and/or thawing,...
Short-term preservation of canine preantral follicles : effects of temperature,...
Lopes, Cláudio Afonso Pinho; Santo, Regiane Rodrigues dos; Celestino, Juliana Jales de Hollanda; Melo, Mônica Aline Parente; Chaves, Roberta NogueiraThe use of the large pool of preantral follicles is a promising alternative to provide high numbers of fertilizable oocytes to reproductive biotechnology. This issue is particularly important to canids, since current rates of success of in vitro techniques using oocytes are very limited, and many species within this family are threatened by extinction. The aim of this study was to evaluate effects of temperatur...
Preservation of bovine preantral follicle viability and ultra-structure after c...
Celestino, Juliana Jales de Hollanda; Santos, Regiane Rodrigues dos; Lopes, Cláudio Afonso Pinho; Martins, Fabrício Sousa; Matos, Maria Helena TavaresBovine preantral follicles within ovarian fragments were exposed and cryopreserved in absence or presence of 1.5 M glycerol (GLY), ethylene glycol (EG), propanediol (PROH) or dimethyl sulfoxide (DMSO), undergoing a previous cooling at 20 °C for 1 h (protocol 1) or at 4 °C for 24 h (protocol 2) in 0.9% saline solution. At the end of each treatment, preantral follicles were classified as non-viable/viable when th...