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Yarrowia lipolytica uses olive tree prunings hydrolysates for microbial lipids ...

Dias, Bruna Filipa Silva; Rodríguez-Jasso, Rosa M.; Belo, Isabel; Lopes, Marlene

Efficiently valorizing olive tree prunings (OTP) for microbial lipids represents an excellent strategy to achieve resource circularity, accompanied by economic and environmental benefits. Since the production of lipids from OTP hydrolysate (OTPH) by yeasts remains underexplored, this study investigated the potential of Y. lipolytica to produce lipid-rich biomass in bioreactor batch cultures. OTP was fractionate...


Transforming orange and banana peels fermented with Yarrowia lipolytica into nu...

Lopes, Marlene; Araújo, Liliana Patrícia Silva; Fernandes, Andreia; Solinho, Joana; Belo, Isabel; Pinheiro, Rita


Alternative feedstocks for high-quality biodiesel: Lipid production from eucaly...

Dias, Bruna; Lopes, Marlene; Marques, Susana; Gírio, Francisco; Belo, Isabel

ABSTRACT: Microbial lipids produced by yeasts from lignocellulosic biomass are a promising feedstock for the biodiesel industry, providing a renewable energy source as an alternative to traditional fossil fuels. This study investigated the potential of Yarrowia lipolytica W29 to produce lipid-rich biomass from undetoxified sugar-concentrated eucalyptus bark hydrolysate (EBH). The lipid concentrations achieved i...

Date: 2025   |   Origin: Repositório do LNEG

Leveraging fermented fruit peels with Yarrowia lipolytica to develop innovative...

Lopes, Marlene; Araújo, Liliana; Fernandes, Andreia; Solinho, Joana; Belo, Isabel; Pinheiro, Rita

Consumers are increasingly concerned about the nutritional value, safety, and sustainability of new food products. As awareness of health and environmental issues grows, they seek options that provide essential nutrients while being free from harmful additives and produced in environmentally responsible ways. The search for healthier diets has instigated the exponential growth of the plant-based food industry. ...


Alternative feedstocks for high-quality biodiesel: Lipid production from eucaly...

Dias, Bruna; Lopes, Marlene; Marques, Susana; Gírio, Francisco; Belo, Isabel

Abstract Microbial lipids produced by yeasts from lignocellulosic biomass are a promising feedstock for the biodiesel industry, providing a renewable energy source as an alternative to traditional fossil fuels. This study investigated the potential of Yarrowia lipolytica W29 to produce lipid-rich biomass from undetoxified sugar-concentrated eucalyptus bark hydrolysate (EBH). The lipid concentrations achieved in...


Unravelling the potential of Palmaria palmata hydrolysates to produce value-add...

Dias, Bruna Filipa Silva; Lopes, Marlene; Belo, Isabel

Seaweed farming and production have grown significantly, driven by their use for human consumption and the extraction of bioactive compounds with application in the feed, pharmaceutical, cosmetic, and textile industries. Palmaria palmata, a seaweed native to the Atlantic coast of Europe, is widely explored for the extraction of proteins and polyphenols. The residual seaweed biomass obtained after compounds extr...


Boosting insect meal application in food by bioprocessing with yeasts

Araújo, Liliana; Dias, Bruna; Salgado, José Manuel; Lopes, Marlene; Belo, Isabel

The growing global demand for protein-rich foods requires sustainable alternatives to the conventional animal based protein systems. In this sense, insect-based proteins, especially from Tenebrio molitor, present a promising nutrient-rich solution with a reduced ecological footprint. Nonetheless, insect meal is composed of chitin and complex proteins, limiting their digestibility. This study aimed to enhance th...


How customer expectations drive loyalty to food delivery app brands

Marques, Alzira; Lopes, Marlene; Santos, Célia

Consumers frequently use food delivery apps due to time constraints or the desire to explore diverse cuisines. These apps offer convenience and accessibility, but user loyalty is also shaped by factors such as ease of use, performance expectations, information quality, self-efficacy, and user enjoyment. This study analyses how user expectations contribute to brand loyalty in food delivery apps. A quantitative s...

Date: 2025   |   Origin: IC-online

Rice husk, brewer’s spent grain, and vine shoot trimmings as raw materials for ...

Guimarães, Ana Cristina Silva Esperança; Mota, Ana C.; Pereira, Ana Sofia Silva; Fernandes, Margarida; Lopes, Marlene; Belo, Isabel

Solid by-products with lignocellulosic structures are considered appropriate substrates for solid-state fermentation (SSF) to produce enzymes with diverse industrial applications. In this work, brewer’s spent grain (BSG), rice husk (RH), and vine shoot trimmings (VSTs) were employed as substrates in SSF with Aspergillus niger CECT 2088 to produce cellulases, xylanases, and amylases. The addition of 2% (NH4)2SO4...


Yarrowia lipolytica growth, lipids, and protease production in medium with high...

Miranda, Sílvia M.; Belo, Isabel; Lopes, Marlene

Two strains of Yarrowia lipolytica (CBS 2075 and DSM 8218) were first studied in bioreactor batch cultures, under different controlled dissolved oxygen concentrations (DOC), to assess their ability to assimilate aliphatic hydrocarbons (HC) as a carbon source in a mixture containing 2 g·L1 of each alkane (dodecane and hexadecane), and 2 g·L1 hexadecene. Both strains grew in the HC mixture without a lag phase, an...


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