108 documents found, page 1 of 11
Yarrowia lipolytica uses olive tree prunings hydrolysates for microbial lipids ...
Dias, Bruna Filipa Silva; Rodríguez-Jasso, Rosa M.; Belo, Isabel; Lopes, MarleneEfficiently valorizing olive tree prunings (OTP) for microbial lipids represents an excellent strategy to achieve resource circularity, accompanied by economic and environmental benefits. Since the production of lipids from OTP hydrolysate (OTPH) by yeasts remains underexplored, this study investigated the potential of Y. lipolytica to produce lipid-rich biomass in bioreactor batch cultures. OTP was fractionate...
Solid-state fermentation by Yarrowia lipolytica improves the nutritional value ...
Araújo, Liliana; Fernandes, Andreia Rafaela Sá; Belo, Isabel; Pinheiro, Rita; Lopes, MarleneThe awareness and concern about dietary choices have been growing among consumers, leading to a surge in demand for information regarding food quality, sustainability, and safety [1,2]. The search for healthier diet habits has instigated the exponential growth of the organic food industry [3]. Therefore, fermentation emerged as a promising strategy to improve the nutritional profile of food products (such as pr...
Rice husk, brewer’s spent grain, and vine shoot trimmings as raw materials for ...
Guimarães, Ana Cristina Silva Esperança; Mota, Ana C.; Pereira, Ana Sofia Silva; Fernandes, Margarida; Lopes, Marlene; Belo, IsabelSolid by-products with lignocellulosic structures are considered appropriate substrates for solid-state fermentation (SSF) to produce enzymes with diverse industrial applications. In this work, brewer’s spent grain (BSG), rice husk (RH), and vine shoot trimmings (VSTs) were employed as substrates in SSF with Aspergillus niger CECT 2088 to produce cellulases, xylanases, and amylases. The addition of 2% (NH4)2SO4...
Bioprocessamento de produtos derivados de insetos pela leveduraYarrowia lipolytica
Dias, Bruna Filipa Silva; Araújo, Liliana Patrícia Silva; Araújo, Joana Isabel; Salgado, José Manuel Seara; Venâncio, Armando; Lopes, MarleneAtualmente, a procura por novas fontes alimentares está a crescer de forma significativa, com o objetivo de responder à expectável e crescente necessidade de alimentos nos próximos anos. Nesse sentido, a FAO (Organização das Nações Unidas para Alimentação e Agricultura) e a União Europeia têm promovido a incorporação de insetos como uma alternativa de proteína sustentável e altamente nutritiva para o consumo hu...
Valorização de cascas de fruta por fermentação sólida com Y. lipolytica
Fernandes, Andreia Rafaela Sá; Araújo, Liliana Patrícia Silva; Rebelo, Ana Isabel Castro Sampaio; Lopes, MarleneA preocupação com a qualidade e segurança alimentar tem crescido entre os consumidores, impulsionando o crescimento da indústria de alimentos biológicos [1]. A fermentação microbiana apresenta-se como uma estratégia promissora para melhorar o perfil nutricional dos produtos alimentares, aumentando o conteúdo em proteína e reduzindo a quantidade de açúcares totais. O projeto Micro4Food (2022.01705.PTDC) tem como...
Enrichment of fruit peels nutritional value by solid-statefermentation with Asp...
Araújo, Liliana Patrícia Silva; Vilela, Helena; Solinho, Joana; Pinheiro, Rita; Belo, Isabel; Lopes, MarleneThe fruit processing industry is responsible for disposing of huge amounts of byproducts, especially fruit peels (FPs), which are often discarded in landfills. Using FPs in biotechnological processes contributes to a circular economy, reducing the environmental burden of FPs and increasing the revenue of the fruit processing industry. This study was focused on upgrading the nutritional value of orange (OPs) and...
Yarrowia lipolytica growth, lipids, and protease production in medium with high...
Miranda, Sílvia M.; Belo, Isabel; Lopes, MarleneTwo strains of Yarrowia lipolytica (CBS 2075 and DSM 8218) were first studied in bioreactor batch cultures, under different controlled dissolved oxygen concentrations (DOC), to assess their ability to assimilate aliphatic hydrocarbons (HC) as a carbon source in a mixture containing 2 g·L1 of each alkane (dodecane and hexadecane), and 2 g·L1 hexadecene. Both strains grew in the HC mixture without a lag phase, an...
Unraveling the potential of Yarrowia lipolytica to utilize wastemotor oil as a ...
Miranda, Sílvia M.; Belo, Isabel; Lopes, MarleneThis study evaluated the potential of Y. lipolytica (CBS 2075 and DSM 8218) to grow in waste motor oil (WMO) and produce valuable compounds, laying the foundation for a sustainable approach to WMO management. Firstly, yeast strains were screened for their growth on WMO (210 g·L1) in microplate cultures. Despite limited growth, the CBS 2075 strain exhibited comparable growth to control conditions (without WMO), ...
Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyp...
Dias, Bruna; Lopes, Marlene; Fernandes, Helena; Marques, Susana; Gírio, Francisco; Belo, IsabelUsing lignocellulosic biomass hydrolysate as a renewable and abundant feedstock for microbial lipids production is a sustainable and economic high-potential approach. This study investigated the potential of the oleaginous yeast Yarrowia lipolytica to produce lipids-rich biomass from eucalyptus bark hydrolysate (EBH) obtained by enzymatic hydrolysis of the biomass pretreated by steam explosion. The effect of EB...
Exploring the use of hexadecane by Yarrowia lipolytica: Effect of dissolved oxy...
Miranda, Sílvia Cristiana Martins; Lopes, Marlene; Belo, IsabelThis work aimed to evaluate the effect of medium composition and volumetric oxygen transfer coefficient (kLa) on Y. lipolytica growth and production of microbial lipids and enzymes from hexadecane. In the stirred tank bioreactor, increasing kLa from 11 h 1 to 132 h 1 improved the hexadecane assimilation rate, biomass concentration, and lipids synthesis (0.90 gL 1). A cost-effective hexadecane-based medium suppl...