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Building a Portuguese coalition for biodiversity genomics

Marques, João; Alves, Paulo; Amorim, Isabel; Lopes, Ricardo; Moura, Mónica; Meyers, Eugene; Sim-sim, Manuela; Sousa-Santos, Carla; Alves, Judite

The diverse physiography of the Portuguese land and marine territory, spanning from continental Europe to the Atlantic archipelagos, has made it an important repository of biodiversity throughout the Pleistocene glacial cycles, leading to a remarkable diversity of species and ecosystems. This rich biodiversity is under threat from anthropogenic drivers, such as climate change, invasive species, land use changes...


Brucellosis in Portugal: A systematic review and meta-analysis of animal and hu...

Lopes, Ricardo; Lima de Carvalho, Hugo; Leclerc Duarte, Elsa; Coelho, Ana Claudia; Cardoso, Luis Lucas; Garces, Andreia

Brucellosis remains one of the most widespread zoonoses globally, yet its true burden in Portugal remains insufficiently characterised1,2,3,4. This study presents the first nationwide systematic review and meta-analysis of Brucella spp. infections across both animal and human populations in Portugal, following PRISMA and MOOSE guidelines. A total of 871 studies were screened, yielding 62 eligible reports publis...


What do we know about Cryptococcus spp. in Portugal?

Lopes, Ricardo; Barros, Gonçalo; Garces, Andreia; Cardoso, Luis; Silva, Vanessa; Lima de Carvalho, Hugo; Leclerc Duarte, Elsa; Coelho, Ana Cláudia

Cryptococcosis is an environmentally acquired mycosis primarily caused by the Cryptococcus neoformans and C. gattii species complexes. These encapsulated yeasts infect humans and multiple animal hosts. In veterinary medicine their importance has gained recognition in recent decades, and affected animals can act as sentinels of environmental exposure within a One Health/Planetary Health framework (Figure 1). In ...


Seroepidemiology of Rickettsia conorii in dogs in Portugal: a comprehensive 12-...

Lopes, Ricardo; Carvalho, Hugo L; Garces, Andreia; Fernandes, Cátia; Lopes; Martins, A; Duarte, Elsa Leclerc; Cardoso, Luis Lucas; Coelho, AC

Background Mediterranean spotted fever (MSF), caused by Rickettsia conorii, is a zoonotic tick-borne disease of important public health concern, particularly in the Mediterranean Basin. Dogs serve as key sentinels for MSF due to their exposure to vector ticks and close contact with humans. To date, no comprehensive study in Portugal has investigated epidemiological risk factors in dogs infected with or exposed ...


Pseudomonas aeruginosa: One Health approach to deciphering hidden relationships...

de Sousa, Telma; Machado, Sandro; Caniça, Manuela; Ramos, Miguel J.N.; Santos, Daniela; Ribeiro, Miguel; Hébraud, Michel; Igrejas, Gilberto

Aims: Antimicrobial resistance in Pseudomonas aeruginosa represents a major global challenge in public and veterinary health, particularly from a One Health perspective. This study aimed to investigate antimicrobial resistance, the presence of virulence genes, and the genetic diversity of P. aeruginosa isolates from diverse sources. Methods and results: The study utilized antimicrobial susceptibility testing, g...


Dermatophytosis in Companion Animals in Portugal: A Comprehensive Epidemiologic...

Lopes, Ricardo; Garcês, Andreia; Silva, Augusto; Brilhante-Simões, Paula; Martins, Angela; Cardoso, Luís; Duarte, Elsa Leclerc; Coelho, Ana Cláudia

Dermatophytosis, commonly referred to as ringworm, is a common superficial fungal infection in companion animals and humans. Between 2012 and 2023, plucked hair and scraped scale samples from domestic dogs and cats with clinical suspicion of dermatophytosis were collected from 355 veterinary medical centres across mainland Portugal. A total of 4716 animal samples were inoculated onto DERM agar, incubated at 25 ...


Feline Infectious Peritonitis Effusion Index: A Novel Diagnostic Method and Val...

Lopes, Ricardo; Sampaio, Filipe; Lima de Carvalho, Hugo; Garcês, Andreia; Fernandes, Cátia; Neves, Carolina Vitória; Sardinha de Brito, Alexandre

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led the medical and scientific community to explore the pathogenesis and clinical manifestations of coronaviruses. In felines, a widespread coronavirus known as feline coronavirus (FCoV) can lead to feline infectious peritonitis (FIP), a highly fatal disease characterised by severe systemic inflammation. Diagnosing FCoV remains ch...


First description of antifungal susceptibility of yeast-like fungi in dermal le...

Lopes, Ricardo; Garcês, Andreia; Silva, Augusto; Brilhante-Simões, Paula; Martins, Ângela; Cardoso, Luís; Duarte, Elsa Leclerc; Coelho, Ana Cláudia

Yeast infections, particularly those caused by species of the genera Malassezia and Candida, are important contributors to otitis and dermatitis in dogs. This study investigates the prevalence and antifungal resistance of various yeast-like fungi isolated from canine dermal lesions in Portugal. Between 2018 and 2023, samples from dogs with clinically suspected yeast infections were collected and analysed using ...


Distribution of and Relationships between Epidemiological and Clinicopathologic...

Lopes, Ricardo; Garcês, Andreia; Silva, Augusto; Brilhante-Simões, Paula; Martins, Ângela; Duarte, Elsa Leclerc; Coelho, Ana Cláudia; Cardoso, Luís

Leishmaniosis is a vector-borne disease caused by protozoan parasites of the genus Leishmania, which are zoonotic and have an important impact on animal and public health globally. Between 2009 and 2023, blood samples from domestic dogs with clinical suspicion of leishmaniosis were received from 286 veterinary medical centres throughout mainland Portugal. An enzyme-linked immunosorbent assay (ELISA) was utilise...


First description of antifungal susceptibility of yeast-like fungi in dermal le...

Lopes, Ricardo; Garcês, Andreia; Leclerc Duarte, Elsa; Cardoso, Luis; Coelho, Ana Cláudia; Brilhante Simoes, Paula

Yeast infections, particularly those caused by species of the genera Malassezia and Candida, are important contributors to otitis and dermatitis in dogs. This study investigates the prevalence and antifungal resistance of various yeast-like fungi isolated from canine dermal lesions in Portugal. Between 2018 and 2023, samples from dogs with clinically suspected yeast infections were collected and analysed using ...


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